Fluorescence and dynamics of structural environment of TRP125 fluorophore in ЕМАР II cytokine
Keywords:
ЕМАР II cytokine, protein dynamics, fluorescence
Abstract
The EMAP II cytokine is a multifunctional polypeptide, which reveals dual activities both as a cytokine and a tRNA-binding domain. The intramolecular dynamics of EMAP II cytokine in solution was studied using the methods of fluorescence spectroscopy. The spectrum of tryptophan fluorescence of EMAP II cytokine is caused by the emission of a single Trp125 in protein. The microenvironment of tryptophan residue has been characterized using the fluorescence spectroscopy data and by computer analysis of protein 3D structure. The accessibility of Trp125 in protein globule is about 10%. The rapid conformational dynamics of EMAP II has been revealed using the enhanced quenching of Trp125 fluorescence by acrylamide due to the protein matrix fluctuation in nanosecond time scale.
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References
Kao J, Ryan J, Brett G, Chen J, Shen H, Fan YG, et al. Endothelia-monocyte activating polypeptide II. J. Biol. Chem. 1992;267(28):20239-47.
Kao J, Houck K, Fan Y, Haehnel I, Libutti SK, Kayton ML, et al. Characterization of a novel tumor-derived cytokine Endothelia-monocyte activating polypeptide II. J. Biol. Chem. 1994;269:25106019.
Schwarz MA, Kandel J, Brett J, Li J, Hayward J, Schwarz RE, et al. Endothelia-monocyte activating polypeptide II, a novel antitumor cytokine that suppresses primary and metastatic tumor growth and induses apoptosis in growing endothelial cell. J. Exp. Med. 1999;190(3):4066-9.
Marvin MR, Libutti SK, Kayton M, Kao J, Hayward J, Grikscheit T, et al. A novel tumor-derived mediator that sensitizes cytokine-resistant to tumors to tumors necrosis factor. J. Surg. Res. 1996;24:248-55.
Berger AC, Tang G, Alexander HR, Libutti SK. Endothelia-monocyte activating polypeptide II induces endothelial cell apoptosis and may inhibit tumor angiogenesis. Microvasc. Res. 2000;60:70-80.
Shalak V, Kaminska M, Mitnaht-Kraus R, Vandenabeele P, Clauss M, Mirande M. The EMAP II cytokine is released from the mammalian multysynthetase complex after cleavage of its p43/proEMAP II component. J. Biolog. Chem. 2001;276(26):23769-76.
Mirande M. Aminoacyl-tRNA synthetase family from prokaryotes and eukaryotes: structural domains and their implications. Prog. Nucleic Acid Res. Mol. Biol. 1991;40:95-142.
Levanetc OV, Naidenov VG, Odynetc KA, Vudmaska MI, Matcuka GKh, Korneliuk AI. Gomologiia S-kontcevogo nekataliticheskogo domena tirozil-tRNK sintetazy mlekapitaiushchikh s tcitokinom EMAP II i nekataliticheskimi domenami metionil- i fenilalanil-tRNK sintetaz. Biopolimery i kletka. 1997;13(6):474-478. (in Russian)
Kornelyuk A, Maarten P, Dubrovsky A, Murray J. Cytokine activity of non-catalytic EMAP II-like domain of mammalian tyrosyl-tRNA synthetase. Biopolimery I kleitka. 1999;15(2):168-72.
Chihade V, Schimmel P. Assembly of a catalytic unit for RNA microhelix aminoacylation using nonspecific RNA binding domains. Proc. Natl. Acad. Sci. USA. 1999;96:12316-21.
Wang CC, Schimmel P. Species barrier to RNA recognition overcome with nonspecific RNA binding domains. J. Biol. Chem. 1999;274:16508-12.
Lakkowicz JR. Principles of Fluorescent Spectroscopy. New York: Plenum Press; 1999.
Ladokhin AS. Fluorescence spectroscopy in peptide and protein analysis. Encyclopedia of Analytical Chemistry. Chichester: John Wiley & Sons Ltd; 2000.
Dubrovskii AL, Braun Dzh, Korneliuk AI, Miurrei K, Matcuka GKh. Bakterialnaia ekspressiia polnorazmernykh i usechennykh form tcitokina EMAR II i tcitokinopodobnogo domena tirozil-tRNK sintetazy mlekoptaiushchikh. Biopolimery i kletka. 2000;16(3):229-35. (in Russian)
Ehrenberg M, Cronvall E, Rigler R. Fluorescence of proteins interacting with nucleic acids: Correction for light absorbtion. FEBS Lett. 1971;18(2):199-203.
Renault L, Kerjan P, Pasqualato S, Menetrey J, Robinson JC, Kawaguchi S, et al. Structure of the EMAP II domain of human aminoacil-tRNA synthetase complex reveals evolutionary dimer mimicry. EMBO J. 2001;20(3):570-8.
Kim Y, Shin J, Li R, Cheong C, Kim K, Kim S. A novel anti-tumor cytokine contains a RNA-binding motif present in aminoacyl-tRNA synthetases. J. Biol. Chem. 2000;275:27062-8.
Burshtein EA. Sobstvennaia liuminestcentciia belka. Priroda i primenenie. Itogi nauki i tekhniki. Biofizika. 1977;7:189-95.
Burstein EA, Vedenkina NS, Ivkova MN. Fluorescence and the location of tryptophan residues in protein molecules. Photochem. Photobiol. 1973;18(2):263-79.
Kantor Ch, Shimmel P. Biofizicheskaia khimiia. Moskva: Mir; 1984. 336p.
Chen Y, Barkley MD. Toward understanding tryptophan fluorescence in proteins. Biochemistry. 1998;37(28):9976-82.
Kao J, Houck K, Fan Y, Haehnel I, Libutti SK, Kayton ML, et al. Characterization of a novel tumor-derived cytokine Endothelia-monocyte activating polypeptide II. J. Biol. Chem. 1994;269:25106019.
Schwarz MA, Kandel J, Brett J, Li J, Hayward J, Schwarz RE, et al. Endothelia-monocyte activating polypeptide II, a novel antitumor cytokine that suppresses primary and metastatic tumor growth and induses apoptosis in growing endothelial cell. J. Exp. Med. 1999;190(3):4066-9.
Marvin MR, Libutti SK, Kayton M, Kao J, Hayward J, Grikscheit T, et al. A novel tumor-derived mediator that sensitizes cytokine-resistant to tumors to tumors necrosis factor. J. Surg. Res. 1996;24:248-55.
Berger AC, Tang G, Alexander HR, Libutti SK. Endothelia-monocyte activating polypeptide II induces endothelial cell apoptosis and may inhibit tumor angiogenesis. Microvasc. Res. 2000;60:70-80.
Shalak V, Kaminska M, Mitnaht-Kraus R, Vandenabeele P, Clauss M, Mirande M. The EMAP II cytokine is released from the mammalian multysynthetase complex after cleavage of its p43/proEMAP II component. J. Biolog. Chem. 2001;276(26):23769-76.
Mirande M. Aminoacyl-tRNA synthetase family from prokaryotes and eukaryotes: structural domains and their implications. Prog. Nucleic Acid Res. Mol. Biol. 1991;40:95-142.
Levanetc OV, Naidenov VG, Odynetc KA, Vudmaska MI, Matcuka GKh, Korneliuk AI. Gomologiia S-kontcevogo nekataliticheskogo domena tirozil-tRNK sintetazy mlekapitaiushchikh s tcitokinom EMAP II i nekataliticheskimi domenami metionil- i fenilalanil-tRNK sintetaz. Biopolimery i kletka. 1997;13(6):474-478. (in Russian)
Kornelyuk A, Maarten P, Dubrovsky A, Murray J. Cytokine activity of non-catalytic EMAP II-like domain of mammalian tyrosyl-tRNA synthetase. Biopolimery I kleitka. 1999;15(2):168-72.
Chihade V, Schimmel P. Assembly of a catalytic unit for RNA microhelix aminoacylation using nonspecific RNA binding domains. Proc. Natl. Acad. Sci. USA. 1999;96:12316-21.
Wang CC, Schimmel P. Species barrier to RNA recognition overcome with nonspecific RNA binding domains. J. Biol. Chem. 1999;274:16508-12.
Lakkowicz JR. Principles of Fluorescent Spectroscopy. New York: Plenum Press; 1999.
Ladokhin AS. Fluorescence spectroscopy in peptide and protein analysis. Encyclopedia of Analytical Chemistry. Chichester: John Wiley & Sons Ltd; 2000.
Dubrovskii AL, Braun Dzh, Korneliuk AI, Miurrei K, Matcuka GKh. Bakterialnaia ekspressiia polnorazmernykh i usechennykh form tcitokina EMAR II i tcitokinopodobnogo domena tirozil-tRNK sintetazy mlekoptaiushchikh. Biopolimery i kletka. 2000;16(3):229-35. (in Russian)
Ehrenberg M, Cronvall E, Rigler R. Fluorescence of proteins interacting with nucleic acids: Correction for light absorbtion. FEBS Lett. 1971;18(2):199-203.
Renault L, Kerjan P, Pasqualato S, Menetrey J, Robinson JC, Kawaguchi S, et al. Structure of the EMAP II domain of human aminoacil-tRNA synthetase complex reveals evolutionary dimer mimicry. EMBO J. 2001;20(3):570-8.
Kim Y, Shin J, Li R, Cheong C, Kim K, Kim S. A novel anti-tumor cytokine contains a RNA-binding motif present in aminoacyl-tRNA synthetases. J. Biol. Chem. 2000;275:27062-8.
Burshtein EA. Sobstvennaia liuminestcentciia belka. Priroda i primenenie. Itogi nauki i tekhniki. Biofizika. 1977;7:189-95.
Burstein EA, Vedenkina NS, Ivkova MN. Fluorescence and the location of tryptophan residues in protein molecules. Photochem. Photobiol. 1973;18(2):263-79.
Kantor Ch, Shimmel P. Biofizicheskaia khimiia. Moskva: Mir; 1984. 336p.
Chen Y, Barkley MD. Toward understanding tryptophan fluorescence in proteins. Biochemistry. 1998;37(28):9976-82.
Published
2018-06-25
Cited
How to Cite
Kordysh, M. A., & Kornelyuk, A. I. (2018). Fluorescence and dynamics of structural environment of TRP125 fluorophore in ЕМАР II cytokine. Biophysical Bulletin, 2(13), 38-42. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/12370
Section
Molecular biophysics
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