Determination of the activity of T-lymphocytes activated by bronchopulmonary antigens and their immunodiagnostic significance in children with bronchial asthma

Abstract

ABSTRACT. It is known that under the influence of specific mitogens, sensitized T-lymphocytes produce № 199 cytokines in the culture medium, which are divided into two fractions: F1 and F2. The first (F1) of them determines the pathogenesis of AD in children and the cytokines of this fraction are produced during immunopathological reactions, and the second (F2) is responsible for the immunological parameters of activation of the T-system of immunity and their regulation. Goal. To investigate the functional activity of T-lymphocytes activated by bronchopulmonary antigens and its differential immunodiagnostic value in children with BA. Materials and methods. To determine the cytokines of sensitized T-lymphocytes, the qualitative composition of cytokines of T-lymphocytes in the culture fluid, discphoresis in polyacrylamide gel was used in 105 children with asthma and 20 healthy children aged 5 to 14 years with specific antigens of bronchi and lung tissue, as well as with non-specific myogen FHA from the company «Reanal». Results. In BA in children, the more quantitative presence of bands and discforograms is detected depending on the severity of the course of the disease and is characterized by high production of F1-fraction cytokines by sensitized T-lymphocytes. The structural analysis of cytokine disc-phorograms of T-lymphocytes in AD under the conditions of mitogenic activity of sensitized T-lymphocytes switched with lipopolysaccharide antigens of the bronchi and lung tissue showed discrepancies in the form of the presence of two bands that have qualitative differences determined by the thickness, with the total numbering from the start having the serial number 8 and 9. Conclusions. It has been established that the severity of BA correlates with the expression of the spectrum of cytokine production of sensitized T-lymphocytes in the blood serum of children with BA, and allows diagnosing the severity of the disease with high accuracy.