Clinical and experimental studies of tissues in thermal injuries
Abstract
The article is devoted to studies used in the clinic and in the experiment in the study of tissue damage caused by exposure to high temperature. Determining the area and depth of tissue damage during thermal injury is of fundamental importance not only for treatment, but also for prognosis. The use of laser Doppler flowmetry, magnetic resonance imaging, pH-metry, contactless infrared thermometry of burn wounds, the method of assessing tissue viability based on the study of dielectric parameters allows to evaluate the viability and the state of damaged tissues in dynamics. The importance of tissue research methods for establishing an accurate diagnosis, assessing the readiness of wounds for autodermoplasty and determining the extent of surgical interventions is shown. The use of morphological methods in experimental studies and in the treatment of victims with burns is substantiated. Using histological and cytological methods, the condition of the burn wound is monitored, the efficacy of surgical interventions and the use of therapeutic agents is evaluated, the wound process phase is determined, and medical tactics is planned. The histochemical method is used to study the composition and state of the tissues of a burn wound paranecrosis area, and the immunohistochemical method is used to assess the intensity of regeneration processes by determining the tissue proliferation and differentiation markers. Ultramicroscopic examination of tissues at different times after thermal injury is informative for understanding the dynamics of pathomorphological changes in burns. Review of the pathogenesis of burn disease, taking into account the data of ultramicroscopic studies, creates the prospect of developing methods for targeted correction of the condition of patients with burns at the subcellular level. The role of experimental studies in the investigation of thermal damage, preclinical trials of medicinal products used to influence the wound process, improving the existing methods and developing the new ones for treatment of burn injury has been determined.
Downloads
References
Boyko V.V., Kozin Yu.I., Oleynik G.A. et all. (2014) Prospects for limiting the depth of burn injury and activating reparative processes in the wound. «Klinichna Khirurgiia» journal. 11.3(867). P.44. [in Ukrainian]
Boiko V.V., Nevzorov V.P., Nevzorova O.F., Zamyatin P.N., Omelchenko V.F., Protsenko E.S., Remnyova N.A. (2016) Submicroscopic restructuring in renal nephron cells of rats with simulated thermal burns. Journal Bulletin of problems biology and medicine. 1(1). P.264-269. [in Russian]
Borys R.Ya. (2009) Microstructure different areas of white rat skin in a norm. Scientific bulletin of Uzhhorod university, series «Medicine». 35. P.8-11. [in Ukrainian]
Havryliuk-Skyba H.O., Volkov K.S. (2013) Submicroscopial changes of the spleen’s structural components in early stages after burn injuries in experiment. Journal World of Medicine and Biology. 1. P. 112-116. [in Ukrainian]
Getmanyuk I.B., Volkov K.S. (2010) Ultrastructural сhanges in the auricles and ears of the heart experimental thermal trauma. Journal World of Medicine and Biology. 1. P. 57-60. [in Ukrainian]
Golubinskaya E.P. (2010) Immunohistochemical characteristic of the effectiveness of the preparation of the bronchoalveolar protective complex during thermal and chemical burns of the skin. Materials of the scientific practical conference of pathologists of Ukraine "Actual problems of modern pathomorphology". P.31-32. [in Russian]
Grigoryeva T.G. (2000) Burn disease. 6(2). P.53-60. [in Russian]
Ivanova Yu.V., Boiko V.V., Krivoruchko I.A., Zamyatin P.N., Isaev Yu.I., Kravtsov A.V., Mushenko E.V., Ivanov V.K., Stadnik A.M., Tkach S.V. (2015) Experimental study assessing tissue viability on the basis of determining the dielectric parameters. Kharkiv surgical school. 3(72). P.112-117. [in Russian]
Коvalenko A.O. (2015) Thermometry application for the skin burns depth. «Klinichna Khirurgiia» journal. 4. P.66-68. [in Ukrainian]
Kovalenko О. N., Kovalenko A.O., Omelchenko A. V. (2015) Мeasurement PH burn wound defines indications for early surgical treatment. «Klinichna Khirurgiia» journal. 11.2. P.39-41. [in Ukrainian]
Kozhemyakin Yu.M., Chromov O.S. M.A.Filonenko et all. (2017) Scientific and practical recommendations for the for the care and use of laboratory animals. Interservis, Kiev. 179 p. [in Ukrainian]
Kozinets G.P. (2010) Basic principles of the organization and provision of assistance to patients with thermal damage to the skin. Health of Ukraine.3. P.14. [in Russian]
Kozinets G.P., Voronin A.V. (2014) New concept of the development of combiustrial service in Ukraine. Scientific reviewed journal «Bulletin of urgent and recovery surgery». 1 (15). P.6. [in Ukrainian]
Kornienko V.V., Oleshko O.M. (2014) The Features of Morphogenesis of a Burn Wound Applying Chitosan Films in Elderly Animals. Journal Bulletin of problems biology and medicine. 4.1(113). P.275-280. [in Ukrainian]
Korobeynikova E.P., Komarova E.F. (2016) Laboratory animals - biomodels and test systems in fundamental and preclinical experiments in accordance with standards of proper laboratory practice (GLP). Jurnal fundamental medicine and biology. 1. Р.30-36. [in Russian]
Кravtsov А. V., Boyko V. V., Kozin Yu. I., Logachov V. К., Isaev Yu. I., Kanishcheva I. N. (2017) Peculiarities of etstimation of the burn damage, using the maget resonance tomography method. «Klinichna Khirurgiia» journal. 2. P.34-37. [in Russian]
Kramar S. B., Volkov K. S., Kotyk A.A. (2014) Histological and histochemical сhanges of the damaged area of skin in the dynamics after experimental thermal trauma. Journal World of Medicine and Biology. 4(46). P.182-185. [in Ukrainian]
Makarova O.I., Сhaikovsky Yu.B. (2014) Features of ultrastructural longstrem changes in the pulmonary respiratory tract of rats following the thermal injury when corrected by colloidal hyperosmolar Iinfusion solution HAES-LX-5%. Journal Bulletin of problems biology and medicine. 4(46). P.115-120. [in Ukrainian]
Andreev S. et all. (1973) Modeling of diseases. Medicine, Moscow. P.59-76. [in Russian]
Mykha S.Yu., Volkov K.S., Nebesna Z.M., Kramar S.B. (2016) Electronmicroscopic changes of the testes at the early stages after experimental thermal ingury. Morphfologia. 10(3). P.208-211. [in Ukrainian]
Nagaichuk V.I., Khimich S.D., Zheliba M.D., Zhuchenko O.P., Povoroznik A.M., Prysyazhnyuk M.B., Zelenko V.O., Girnik I.S. (2017) Modern technologies of treatment of patients with critical and supercritical burns. Reports of Vinnytsia National Medical University. 21(2). P.428-433. [in Ukrainian]
Kozinets. G.P., Slesarenko S.V., Sorokina O.Yu. et all. (2008) Burned injury and its consequences: A guide for practical doctors. The press of Ukraine, Dnepropetrovsk, 224 p. [in Ukrainian]
Ocheretnyuk А.О., Nebesna Z.M., Gunas I.V., Yakovleva О.О., Palamarchuk О.V. (2013) Elerctron microscopics changes respiratory department of the lungs in rats after thermal trauma of the skin under the usage of NaCl solution. Journal World of Medicine and Biology. 2. P.38-41.[in Ukrainian]
Paramonov B.A.Porebskyi Ya.O., Yablonskyi V.H. (2000) Burns:А gluide for doctors. SpetsLit, St. Peterburg, 480 p. [in Russian]
Podoynitsina M.G., Tsepelev V.L., Stepanov A.V., Kryukova V.V. (2016) Chanches in burning wound in application of magneto-plasmatic therapy. Grekov's Bulletin of Surgery. 175(2). P.49-52. [in Russian]
On Approval of Protocols for the Provision of Medical Aid to Patients with Burns and their Consequences. Ministry of Health of Ukraine (MoH), Order No. 691 dated 07.11.2007. [Electronic resourse] // Access mode: http://zakon.rada.gov.ua/rada/show/v0691282-07. - Access date: 07.11.2007. [in Ukrainian]
Protsenko Е. S., Kravtsov А. V., Remnyova N. А., Shapoval Е. V., Dolgaya O. V., Sazonova Т. M. (2018) Morphological changes in skin tissues of rats in modeling burn injuries. Ukrainian Journal of Medicine, Biology and Sport. 4(13). P. 44-49. [in Ukrainian]
Reva I.V., Odintsova I.A., Usov V.V., Obydennikova T.N., Reva G.V. (2017) Optimization of surgical approach of treatment in patients with fullthickness thermal burns. Grekov's Bulletin of Surgery. 176(2). P.45-50. [in Russian]
Fistal’ E.Y., Soloshenko V.V. (2017) Substantiation of application of allogenic fibroblasts in treatment of extensive burns. Grekov's Bulletin of Surgery. 176(1). P.42-45. [in Russian]
Fistal’ E.Y., Soloshenko V.V., Fistal’ N.N. et all. (2009) The role of laser Doppler fluvetry in choosing the surgical treatment of children with burns. «Klinichna Khirurgiia» journal. 11-12. P.87-88. [in Russian]
Frolova N.Yu., Mel’nikova T.I., Buryakina A.V., Vishnevskaya E.K., Avenirova E.L., Sivak K.V., Karavaeva A.V. (2009) Methodological approaches to the expersmental investigation of dermotropic drugs. Clinical and experimental pharmacology. 5(72).P.56-60. [in Russian]
Shapoval O. V. (2015) Clinical Aspects of Morphology of Tissues in Paranecrosis Area of Burn Wounds. Journal Bulletin of problems biology and medicine. 2. 4(121).P.276-280. [in Ukrainian]
Yurova Y.V., Shlyk I.V. (2013) Influence of microbial wound dissemination and microcirculation on the results of skin engraftment. Grekov's Bulletin of Surgery. 172(1). P.60-64. [in Russian]
Chatterjee J. S. A critical evaluation of the clinimetrics of laser Doppler as a method of burn assessment in clinical practice / J.S. Chatterjee // J. Burn Care Res. – 2006. – Vol. 27(2). – Р. 123-30.
Droog E. J. Measurement of depth of burns by laser Doppler perfusion imaging / E. J. Droog, W. Steenbergen, F. Sjoberg // Burns. – 2001. – Vol. 27. – Р. 561-568.
Mileski W. J. Serial measurements increase the accuracy of laser Doppler assessment of burn wounds / W.J. Mileski, L. Atiles, G. Purdue [et al.] // J. Burn Care Rehabil. – 2003. – Vol. 24. – Р. 187-191.
