Mutations rate in different lines of drosophila melanogaster after the treatment with caffeine and He-Ne laser radiation
Abstract
The frequency of dominant lethal mutations (DLM) after combined effect of caffeine (1,3,7-trimethylxan- thine) and helium-neon laser radiation in Drosophila has been studied. Caffeine is a natural substance which is presented in many drinks, widely applied in medicine and reveals moderately toxic action and some mutagen- icity/genotoxicity effects; red laser radiation is applied in medicine and can disrupt the processes of DNA and RNA biosynthesis. The aim of this work was to study the characteristics of the mutation process in Drosophila melanogaster under the influence of caffeine and red laser radiation, depending on the genotype.
We used wild type Drosophila melanogaster stocks Canton-S (C-S) and Oregon (Or), carrying an ebony mutation, respectively: ebonyC-S and ebonyOr; and mutant stock ebony. Control flies were grown in standard conditions, and in experiments with caffeine it was added in culture medium in concentration 0.5 mg/ml. Vir- gin flies were exposed to a helium-neon laser light (wavelength of 632,8 nm) with a surface power density of
0.03 mW/cm2, the exposure time was 5 minutes.
The influence of caffeine causes an increase in the frequency of early DLM on 40 % in mutant line ebony, and on 70.5 % in the line ebonyC-S. Irradiation by light of helium-neon laser either reduces the incidence DLM, or has no effect in all lines used in this work. The simultaneous effect of two external factors induces diverse answers in different genotypes: it increases early DLM in line C-S and early and late DLM in line ebony; but has no effect in lines ebonyOr and ebonyC-S. The two-way analysis ANOVA F-test showed that the DLM frequency dependence impact of external factors is characterized by F= 3.87; of genotype F= 5.14; of the combined effects of genotype and external factors F = 5.69. The power of influence of external factors — 8.1 %; of genotype 6.9 %; and of the combined effects of the genotype and two external factors studied — 31.4 %.
Conclusions. Irradiation with laser light had no mutagenic effect on Drosophila, and even revealed anti- mutagenic effect. Caffeine induced the DLM increase, but the combined caffeine and laser light impact increased DLM rate in line ebony and not influenced it in lines: ebonyOr and ebonyC-S.
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References
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