Human buccal epithelium cell response to low intensive neutron radiation
Abstract
Background: The investigation of the low doses of ionizing radiation is still a great importance for identification of the threshold of harmful effect and potential hormetic effect of low doses.
Objectives: The purpose of investigation was to evaluate the stress response in human buccal epithelium cells induced by low intensive neutron radiation.
Materials and Methods: The level of chromatin condensation in interphase nuclei was applied for assessment of the cell reaction to stress. Exfoliated human buccal epithelium cells were collected, placed in the 3.03 mM phosphate buffer solution (pH=7.0) with addition of 2.89 mM CaCl2 and exposed to neutron radiation from 2 Pu-Be sources IBN-17. The heterochromatin granule quantity (HGQ) assessments were done after orcein staining that had been immediately performed after cell exposure to neutron radiation in the dose range from 2.3 mSv to 146.0 mSv. Also the effect of fast neutron radiation in the dose of 11.4 mSv on human buccal epithelium cells was investigated in 1-64 min after exposure. The HGQ in every variant of experiment was assessed in 30 nuclei in 3 separate experiments. The mean HGQ and standard error of mean were assessed in every experiment.
Results: Neutron radiation induced the increase of HGQ. Partially slowed neutrons have less influence on neutron-induced HGQ increase than only fast neutrons especially with 1 min delay after exposure. Fast neutrons induce heterochromatinization in cell samples irradiated with doses 4.6–36.5 mSv. Further increase of dose has led to return of HGQ to control levels. After cell exposure to fast neutron flow (11.4 mSv) the peaks of chromatin condensation were observed for time intervals 2–8 and 32–64 min after cell exposure to radiation.
Conclusions: Qualitative characteristic of neutron radiation (slow/fast neutrons) influences the rate of cell stress response as revealed by chromatin condensation in cell nuclei. It was demonstrated that there is a threshold dose above which cells are able to develop stress response to neutron radiation. The dose-response dependence is non-monotonous and is of wave-like form. Described phenomena may be explained by the effect of hormesis.
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References
Chadwick, K. H. Leenhouts, H. P. (1981). The Molecular Theory of Radiation Biology. Heidelberg: Springer-Verlag Berlin.
Seth, I., Schwartz, J. L., Stewart, R. D. (2014). Neutron Exposures in Human Cells: Bystander Effect and Relative Biological Effectiveness. PLoS One, 2014, 9(6), e98947.
Stewart, R. D, Streitmatter, S. W., Argento, D. C. (2015). Rapid MCNP simulation of DNA double strand break (DSB) relative biological effectiveness (RBE) for photons, neutrons, and light ions. Phys. Med. Biol., 60, 8249–8274.
Falusi, O. A., Daudu, O. A. Y., Teni, K. J. (2014). The effect of fast neutron radiation on meiosis in pollen mother cells of Capsicumannuum var. abbreviatum. The International Journal of Plant Reproductive Biology, 6(1), 31–34.
Barendsen, G. W., Broerse, J. J. (1969). Experimental radiotherapy of a rat rhabdomyosarcoma with 15 MeV neutrons and 300 kV X-rays. European Journal of Cancer, 5, 373–391.
Ng, C. Y., Kong, E. Y., Konishi, T. (2015). Low-dose neutron dose response of zebra fish embryos obtained from the neutron exposure accelerator system for biological effect experiments (NASBEE) facility. Radiation Physics and Chemistry, 114, 12–17.
Saeed, A., Raouf, G. A., Nafee, S. S. (2015). Effects of Very Low Dose Fast Neutrons on Cell Membrane and Secondary Protein Structure in Rat Erythrocytes. PLoS One, 10(10), e0139854. https://doi.org/10.1371/journal.pone.0139854
Zhang, J., He, Y., Shen X. (2016). γ-H2AX responds to DNA damage induced by long-term exposure to combined low-dose-rate neutron and γ -ray radiation. Mutation Research, 795, 36–40.
Zhang, D. Q., Liu, Q. J., Zhang, Q. Z. (2015). Dose-effect of ionizing radiation-induced PIG3 gene expression alteration in human lymphoblastoid AHH-1 cells and human peripheral blood lymphocytes. International Journal of Radiation Biology, 91(1), 71–80.
Chang, G. M., Gao, Y. B., Wang, S. M. (2015). Protecting intestinal epithelial cell number 6 against fission neutron irradiation through NF-кB signaling pathway. BioMed Research International, 124721. doi.10.1155/2015/124721.
Miyatake, S., Kawabata, S., Hiramatsu, R. (2016). Boron Neutron Capture Therapy for Malignant Brain Tumors. Neurol Med Chir (Tokyo), 56(7), 361-371. doi: 10.2176/nmc.ra.2015-0297.
Kuznetsov, K. A., Kyzym, P. S., Onishchenko, G. M., Berezhnoy, A. Y., Shckorbatov, Y. G. (2015). Chromatin changes under exposure to neutron radiation. Advances in Cell Biology and Biotechnology: Proceedings of the International Conference (p. 83). Lviv, Ukraine.
Shckorbatov, Y. (2012). The state of chromatin as an integrative indicator of cell stress.
In Simpson N. M. (Ed), Stewart V.J. (Ed). New Developments in Chromatin Research, 123–144.
Dumont, J., Euwart, D., Mei, B., Estes, S., Kshirsagar, R. (2016). Human cell lines for biopharmaceutical manufacturing: history, status, and future perspectives. Critical reviews in biotechnology, 36(6), 1110-1122.
Arnette, C., Koetsier, J. L., Hoover, P., Getsios, S., Green, K. J. (2016). In vitro model of the epidermis: connecting protein function to 3D structure. In Methods in enzymology, Academic Press, 569, 287–308.
Demirovic, D., Rattan, S. I. (2011). Curcumin induces stress response and hormetically modulates wound healing ability of human skin fibroblasts undergoing ageing in vitro. Biogerontology, 12(5), 437–444.
Shckorbatov, Y. G., Shakhbazov, V. G., Bogoslavsky, A. M. (1995). On age-related changes of cell membrane permeability in human buccal epithelium cells. Mech. Ageing Develop., 83, 87-90.
Wakeford, R. (2010). The meaning of low dose and low dose-rate. J. Radiol. Prot. 30(1), 1-3.
Hurem, S., Martıґn, L.M., Brede, D., Skjerve, E., Nourizadeh-Lillabadi R., Lind O.C., …Lyche J.L. (2017). Dose-dependent effects of gamma radiation on the early zebrafish development and gene expression. PLoS ONE, 12(6): e0179259. https://doi.org/10.1371/journal.pone.0179259
Suman, S., Kumar, S., Moon, B. H. (2015). Relative Biological Effectiveness of Energetic Heavy Ions for Intestinal Tumorigenesis Shows Male Preponderance and Radiation Type and Energy Dependence in APC1638N/+ Mice. Int. J. Radiation Oncol. Biol. Phys., 95(1), 131–138.
Virhov, A. I., Dudkin, V. E. Kovalev, E. E. (Ed) et al. (1978). Atlas dozovyh harakteristik vneshnego ionizirujushhego izluchenija: Spravochnik. Moscow : Atomizdat (in Russian).
Batani, D., Conti, A., Masini, A., Milani, M., Costato, M., Pozzi, A., Triglia, A. (1996). Biosystem response to soft-X-rays irradiation: non-monotonic effects in the relevant biological parameters of yeast cells. Il Nuovo Cimento D, 18(5), 657–662.
Joshi, G.S., Joiner, M.C., Tucker, J.D. (2014). Cytogenetic characterization of low-dose hyper-radiosensitivity in Cobalt-60 irradiated human lymphoblastoid cells. Mutation Research, 770, 69–78.
Kudryasheva, N. S., Rozhko, T. V. (2015). Effect of low-dose ionizing radiation on luminous
marine bacteria: radiation hormesis and toxicity. J. Environ Radioact. 142, 68–77. doi: 10.1016/j.jenvrad.2015.01.012.
Murley, J. S., Arbiser, J. L., Weichselbaum, R. R., Grdina, D. J. (2018). ROS Modifiers and NOX4 Affect the Expression of the Survivin-Associated Radio-Adaptive Response. Free Radical Biology and Medicine. https://doi.org/10.1016/j.freeradbiomed.2018.04.547
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