Changes in immunoreactivity in patients with purulent-septic wounds of the lower extremities against the background of type ii diabetes mellitus after light exposure
Abstract
Introduction. Actual is the use of light exposure of different ranges to enhance local regenerative processes in purulent wounds against the background of diabetes mellitus, since standard antibiotic regimens are not always eff ive and require long-term use. Mechanisms of adaptogenesis, activated against the background of light exposure using a photosensitizer, have a significant effect on the processes of cellular and humoral immunity.
The aim of the work was to study the immunocorrecting effects of phototherapy of different wavelengths of radiation (470, 530, 630 nm) using a photosensitizer in the complex treatment of purulent wounds against the background of diabetes mellitus.
Materials and methods. Groups of examined patients with purulent wounds on the background of uncompensated course of type II diabetes were formed in accordance with the treatment. The main group included patients with purulent-septic wounds of the lower extremities against the background of type II diabetes mellitus, which was phototherapy with the photosensitizer Dimegin, and the comparison group included patients with purulent-septic wounds of the lower extremities against the background of type II diabetes mellitus A standard antimicrobial treatment regimen without phototherapy was performed. Irradiation of the pathology zone was carried out with photon matrices Korobov A.-Korobov V. series «Barva».
Patients with purulent-septic wounds of the lower extremities against the background of type II diabetes mellitus were assessed for the C-peptide and glycosylated hemoglobin HbA1c. In the in vitro model, oxygen-dependent and oxygen-dependent (NST-test) phagocytosis, lymphocytotoxicity in the Terasaki test, concentration of circulating immune complexes and peptides of average molecular weight were determined.
Results of the research. Patients with purulent wounds on the background of diabetes mellitus had a tendency to decrease glycosylated hemoglobin more pronounced in the group of patients who underwent phototherapy than in the group of patients using standard treatment regimens. The concentration of C-peptide was more pronounced in the group of patients with phototherapy.
The concentration of peptides of average molecular weight decreased after the phototherapy course in the patients of the main group averaged 1.9 times the baseline compared to patients who did not undergo phototherapy.
Conclusions. Combined light exposure with the use of the photosensitizer dimegin on wounds by radiation of light emitting diodes with wavelengths of 470, 530, 630 nm stimulates and regulates the functional state of all phagocytic cells, which is manifested in activation of chemotaxis and endocytosis of neutrophils. Regular light exposure caused a decrease in the concentration of pathogenic circulating immune complexes by reducing the antigenic load and increasing the activity of hydrolytic enzymes that stimulate the release of circulating immune complexes from the body. Thus, light exposure normalized the molecular mechanisms responsible for immunoreactivity and immunoresistance.
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References
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