Bioaccumulation of Selected Metals and Non-Metals in Mycelium and Fruit Bodies of Ectomycorrhizal Fungi

Keywords: bulk soil, mycelium, metals, fruiting bodies, rhizosphere, soil-root interface


Purpose. We attempted to quantify the contribution of wild-growing mycelium of ectomycorrhizal fungi to the soil level of selected metals and non-metals in upper (0−10 cm) layer of forest soil of  boreal forest ecosystems. The content of selected elements were also analyzed and compared in such fractions of soil as bulk soil, rhizosphere and soil-root interface. Specifically we analyzed the content of phosphorus (P), manganese (Mn), iodine (I), chromium (Cr), nickel (Ni), copper (Cu), zinc (Zn), cadmium (Cd), cobalt (Co), mercury (Hg) lead (Pb) and arsenic (As). Methods. The concentration of the elements in the samples (dry weight, d.w.) was determined by the mass spectrometric method (ICP-MS) in the laboratory ALS Scandinavia AB, Luleå according to the method given in Rodushkin et al. [13]. Statistical data processing was performed using dispersion analysis (ANOVA) and Pearson correlation coefficients. Software Minitab (© 2010 Minitab Inc.). Results. It has been shown that concentration of phosphorus in the mycelium of fungi is about 1.5 times, and in the fruit bodies is about 7 times higher of that the plant plant tissue (soil+root interface). The concentration of manganese in the mycelium is about the same as in the bulk soil and much lower in the fraction of rhizosphere. Iodine, chromium and nickel are not accumulated, neither the mycelium of fungi nor in their fruitful bodies. Copper, zinc and cadmium are accumulated in both fruit bodies and mycelium of the studied species intensively. The concentration of cadmium in the mycelium is found to be about three times higher than in the bulk soil fraction, and about twice as high as in the fraction of rhizosphere. At such concentrations of cadmium in mycelium, the later may account from 16.2 to 32.3% of the total amount of cadmium in the upper, 0-10 cm layer of forest soils. The content of cobalt and mercury in the mycelium appeared to be somewhat higher in the bulk soil, about the same as in the rhizosphere fraction, and significantly higher than in the soil-root interface fraction. Fungi did not accumulate lead neither in the mycelium nor in their fruit bodies, whereas arsenic does not accumulated in soil-root interface and only weakly accumulated by fungal fruit bodies. Conclusions As a result of the study, it was found that the content of most of the analyzed metals and non-metals in the mycelium of ectomycorrhizal fungi of the upper (0-10 cm) soil enriched with organic matter in the forest ecosystem, except for cadmium and phosphorus, does not exceed 10% of their total amount. At the same time, the content of cadmium in the mycelium of fungi was the highest − 16.2 to 32.3%, which indicates the ability of fungi to accumulate this metal. It is suggested that the percentages of the content of the elements studied in the mycelium of upper layers of forest soil is rather underestimated than overestimated.


Download data is not yet available.

Author Biographies

M. M. Vinichuk, Zhytomyr State Technological University

доктор біологічних наук, професор

G. V. Skyba, Zhytomyr State Technological University

кандидат технічних наук, доцент

T. O. Yelnikova, Zhytomyr State Technological University

кандидат технічних наук, доцент

Y. N. Mandro, Zhytomyr State Technological University

асистент кафедри екології


Vіnіchuk, M.M. (2012). Khrom ta nіkel u fraktsіiakh gruntu ta okremykh vydakh makromіtsetіv borealnykh lіsovykh ekosystem.[ Chromium and nickel in soil fractions and certain types of macromycetes of boreal forest ecosystems]. Vіsnyk Zaporіzkoho natsіonalnoho unіversytetu. Bіolohіchnі nauky, 3, 103-110. (In Ukrainian)

Berthelsen B., Olsen R., Steinnes E. (1995). Ectomycorrhizal heavy metal accumulation as a contributing factor to heavy metal levels in organic surface soils. Science of the Total Environment, 170, 141-149.

Blaudez D., Botton B., Chalot M. (2000). Cadmium uptake and subcellular compartmentation in the ectomycorrhizal fungus Paxillus involutus. Microbiology, 146(5), 1109–1117.

Brzostowski A., Jarzyńska G., Kojta A., Wydmańska D., Falandysz J. (2011). Variations in metal levels accumulated in Poison Pax (Paxillus involutus) mushroom collected at one site over four years. Journal of Environmental Science and Health, Part A, 46 (6), 581–588.

Burgess T., Malajczuk N., Grove N. (1993). The ability of 16 ectomycorrhizal fungi to increase growth and phosphorus uptake of Eucalyptus globulus Labill. and E. diversicolor F. Muell., Plant and Soil, 153(2), 155–164.

Byrne A., Ravnik V., Kosta L. (1976). Trace element concentrations in higher fungi. Science of the Total Environment, 6 (1), 65–78.

Gorban G., Clegg S. (1996). A conceptual model for nutrient availability in the mineral soil-root system. Canadian Journal of Soil Science, 76, 125–131.

Janssens I., Sampson D., Curiel-Yuste J., Carrara A., Ceulemans R. (2002). The carbon cost of fine root turnover in a Scots pine forest. Forest Ecology and Management, 168, 231–240.

Lepp N., Harrison S., Morrell B. (1987). A role for Amanita muscaria L. in the circulation of cadmium and vanadium in a non-polluted woodland. Environmental Geochemistry and Health, 9 (3-4), 61–64.

Olsen R. (1994). The transfer of radiocaesium from soil to plants and fungi in semi-natural ecosystems. In: Nordic Radioecology: The transfer of radionuclides through Nordic ecosystems to man. Edited by H. Dahlgaard, 62. Elsevier, Amsterdam, 265–287.

Pérez A., Farías S., Strobl A., Pérez L., López C., Piñeiro A., Roses O., Fajardo M. (2007). Levels of essential and toxic elements in Porphyra columbina and Ulva sp. from San Jorge Gulf, Patagonia Argentina. Science of the Total Environment, 376 (1-3), 51–59.

Read D., Perez-Moreno J. (2003). Mycorrhizas and nutrient cycling in ecosystems – a journey towards relevance? New Phytologist, 157, 475–492.

Rodushkin I., Engström E., Sörlin D., Baxter D. (2008). Levels of inorganic constituents in raw nuts and seeds on the Swedish market. Science of the Total Environment, 392, 290–304.

Smith S. (1997). Mycorrhizal Symbiosis. London, UK, 2nd edition. Academic Press, 605. [In English].

Stijve T., Besson R. (1976). Mercury, cadmium, lead and selenium content of mushroom species belonging to the genus Agaricus. Chemosphere, 5(2), 151–158.

Vinichuk M. (2013). Copper, zinc, and cadmium in various fractions of soil and fungi in a Swedish forest. Journal of Environmental Science and Health, Part A, 4(48), 980–987.

Vinichuk M., Johanson K. (2003). Accumulation of 137Cs by fungal mycelium in forest ecosystems of Ukraine. Journal of Environmental Radioactivity, 64, 27–43.

Vinichuk M., Rosén K., Dahlberg A. (2013). 137Cs in fungal sporocarps in relation to vegetation in a bog, pine swamp and forest along a transect. Chemosphere, 90(2), 713–720.

How to Cite
Vinichuk, M. M., Skyba, G. V., Yelnikova, T. O., & Mandro, Y. N. (2019). Bioaccumulation of Selected Metals and Non-Metals in Mycelium and Fruit Bodies of Ectomycorrhizal Fungi. Visnyk of V. N. Karazin Kharkiv National University Series «Еcоlogy», (20), 23-31.