C60 fullerene improves the recovery of biomechanical parameters of muscle soleus contraction in rats after chronic alcoholization
Abstract
Background: Recovery of biomechanical parameters of contraction of muscles damaged by alcoholic myopathy is a rather complex and long-term process. Therefore, the search for effective therapeutic means for its acceleration is an extremely urgent task in biomedicine.
Aim of work: The effect of C60 fullerene aqueous solution (C60FAS) on the dynamics of skeletal muscle contraction in rats after 9 months of chronic alcoholization and 2 months after cessation of alcohol consumption was investigated.
Materials and Methods: When analyzing the miotic response using tensometry, such biomechanical parameters as the time of reduction of the muscle force response by 50% from the initial value, the values of the contraction force and the impulse of the muscle force were evaluated.
Results: It has been shown that animals orally administered alcohol and C60FAS (daily dose of 1 mg/kg) together (scheme II) during the experiment showed an increase in muscle force response by 40–45±2% compared with the group of alcoholized animals and by 12–15±1% compared with the group of rats administered C60FAS 1 h after alcohol ingestion (scheme I). The positive effect of C60FAS administration according to scheme II and scheme I was 34±2% and 10±1%, respectively, compared with the group of alcoholized animals in the case of recording the time of reduction of the muscle force response by 50% of the initial value. After 2 months of rehabilitation, the level of minimum muscle contraction force when using C60FAS in both schemes differed by about 15±1% compared to the group of alcoholized animals, and the time of reduction of the force response by 50% was not significantly recorded. Finally, the use of C60FAS revealed a significant increase in the magnitude of the muscle force impulse: up to 86±4% (scheme I) and almost to control values — 94±2% (scheme II).
Conclusions: The obtained results indicate the prospects of using C60FAS to restore the biomechanical parameters of skeletal muscle contraction during long-term development of alcoholic myopathy.
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References
Estruch R, Nicolas JM, Villegas E, Junque A, Urbano-Marquez A. Relationship between ethanol-related diseases and nutritional status in chronically alcoholic men. Alcohol Alcohol. 1993;28(5):543–550.
Fernandez-Sola J, Preedy VR, Lang CH, Gonzalez-Reimers E, Arno M, Lin JCI, et al. Molecular and cellular events in alcohol-induced muscle disease. Alcohol Clin Exp Res. 2007;31(12):1953–62. https://doi.org/10.1111/j.1530-0277.2007.00530.x
Hunter RJ, Preedy VR, Neagoe C, Järveläinen HA, Martin CR, Lindros KO, Linke WA. Alcohol affects the skeletal muscle proteins, titin and nebulin in male and female rats. J Nutr. 2003;133(4)1154–7. https://doi.org/10.1093/jn/133.4.1154
Preedy VR, Adachi J, Peters TJ, Worrall S, Parkkila S, Niemela O, et al. Recent advances in the pathology of alcoholic myopathy. Alcohol Clin Exp Res. 2001;25(5):54S–59S. https://doi.org/10.1097/00000374-200105051-00010
Vary TC, Nairn AC, Lang CH. Restoration of protein synthesis in heart and skeletal muscle after withdrawal of alcohol. Alcohol Clin Exp Res. 2004;28(4):517–25. https://doi.org/10.1097/01.alc.0000121653.80502.54
Preedy VR, Adachi J, Ueno Y, Ahmed S, Mantle D, Mullatti N, et al. Alcoholic skeletal muscle myopathy: definitions, features, contribution of neuropathy, impact and diagnosis. Eur J Neurol. 2001;8(6);677–87. https://doi.org/10.1046/j.1468-1331.2001.00303.x
Krusic PJ, Wasserman Е, Keizer PN, Morton JR, Preston KF. Radical reactions of С60. Science. 1991;254(5035):1183–5. https://doi.org/10.1126/science.254.5035.1183
Wang IC, Tai LA, Lee DD, Kanakamma PP, Shen CK, Luh TY, et al. C60 and water-soluble fullerene derivatives as antioxidants against radical-initiated lipid peroxidation. J Med Chem. 1999; 42(22):4614–20. https://doi.org/10.1021/jm990144s
Giust D, Da Ros T, Martín M, Albasanz JL. [60]Fullerene derivative modulates adenosine and metabotropic glutamate receptors gene expression: a possible protective effect against hypoxia. J Nanobiotechnol. 2014;12:27. https://doi.org/10.1186/s12951-014-0027-7
Liu Q, Cui Q, Li XJ, Jin L. The applications of buckminsterfullerene C60 and derivatives in orthopaedic research. Connect Tissue Res. 2014;55(2):71–9. https://doi.org/10.3109/03008207.2013.877894
Nozdrenko DM, Zavodovskyi DO, Matvienko TYu, Zay SYu, Bogutska KI, Prylutskyy YuI, et al. C60 fullerene as promising therapeutic agent for the prevention and correction of functioning skeletal muscle at ischemic injury. Nanoscale Res Lett. 2017;12(1):115. https://doi.org/10.1186/s11671-017-1876-4
Nozdrenko D, Matvienko T, Vygovska O, Bogutska K, Motuziuk O, Nurishchenko N, et al. Protective Effect of Water-Soluble C60 Fullerene Nanoparticles on the Ischemia-Reperfusion Injury of the Muscle Soleus in Rats. Int J Mol Sci. 2021;22(13):6812. https://doi.org/10.3390/ijms22136812
Prylutskyy YuI, Vereshchaka IV, Maznychenko AV, Bulgakova NV, Gonchar OO, Kyzyma OA, et al. С60 fullerene as promising therapeutic agent for correcting and preventing skeletal muscle fatigue. J Nanobiotechnology. 2017;15(1):8. https://doi.org/10.1186/s12951-016-0246-1
Vereshchaka IV, Bulgakova NV, Maznychenko AV, Gonchar OO, Prylutskyy YuI, Ritter U, et al. C60 fullerenes diminish the muscle fatigue in rats comparable to N-acetylcysteine or β-alanine. Front Physiol. 2018;9:517. https://doi.org/10.3389/fphys.2018.00517
Nozdrenko D, Prylutska S, Bogutska K, Nurishchenko N, Abramchuk O, Motuziuk O, et al. Effect of C60 Fullerene on Recovery of Muscle Soleus in Rats after Atrophy Induced by Achillotenotomy. Life (Basel). 2022:12(3);332. https://doi.org/10.3390/life12030332
Nozdrenko D, Matvienko T, Vygovska O, Soroca V, Bogutska K, Zholos A, et al. Post-traumatic recovery of muscle soleus in rats is improved via synergistic effect of C60 fullerene and TRPM8 agonist menthol. Applied Nanoscience. 2022;12(3):467–78. https://doi.org/10.1007/s13204-021-01703-z
Ritter U, Prylutskyy YuI, Evstigneev MP, Davidenko NA, Cherepanov VV, Senenko AI, et al. Structural features of highly stable reproducible C60 fullerene aqueous colloid solution probed by various techniques. Fullerenes, Nanotubes, Carbon Nanostruct. 2015;23(6):530–34. https://doi.org/10.1080/1536383X.2013.870900
Foley S, Crowley C, Smaihi M, Bonfils C, Erlanger BF, Seta P, Larroque C. Cellular localisation of a water-soluble fullerene derivative. Biochem Biophys Res Commun. 2002;294(1):116–9. https://doi.org/10.1016/S0006-291X(02)00445-X
Grebinyk A, Prylutska S, Buchelnikov A, Tverdokhleb N, Grebinyk S, Evstigneev M, et al. C60 fullerene as effective nanoplatform of alkaloid berberine delivery into leukemic cells. Pharmaceutics. 2019;11:586. https://doi.org/10.3390/pharmaceutics11110586
Prylutska SV, Grynyuk II, Grebinyk SM, Matyshevska OP, Prylutskyy YuI, Ritter U, et al. Comparative study of biological action of fullerenes C60 and carbon nanotubes in thymus cells. Materialwissenschaft Werkstofftech. 2009; 40(4): 238–41. https://doi.org/10.1002/mawe.200900433
Tolkachov M, Sokolova V, Loza K, Korolovych V, Prylutskyy Y, Epple M, et al. Study of biocompatibility effect of nanocarbon particles on various cell types in vitro. Materialwissenschaft Werkstofftech. 2016;47(2–3):216–21. https://doi.org/10.1002/mawe.201600486
Prylutska SV, Grebinyk AG, Lynchak OV, Byelinska IV, Cherepanov VV, Tauscher E, et al. In vitro and in vivo toxicity of pristine C60 fullerene aqueous colloid solution. Fullerenes, Nanotubes Carbon Nanostruct. 2019;27:715–28. https://doi.org/10.1080/1536383X.2019.1634055
Ji ZQ, Sun H, Wang H, Xie Q, Liu Y, Wang Z. Biodistribution and tumor uptake of C60(OH)x in mice. J Nanopart Res. 2006;8:53–63. https://doi.org/10.1007/s11051-005-9001-5
Nikolic N, Vranjes-Ethuric S, Jankovic D, Ethokic D, Mirkovic M, Bibic N, Trajkovic V. Preparation and biodistribution of radiolabeled fullerene C60 nanocrystals. Nanotechnol. 2009;20:385102. https://doi.org/10.1088/0957-4484/20/38/385102
Prylutskyy Yu, Nozdrenko D, Gonchar O, Prylutska S, Bogutska K, Tauscher E, et al. The residual effect of C60 fullerene on biomechanical and biochemical markers of the muscle soleus fatigue development in rats. J Nanomater. 2023;2023:2237574. https://doi.org/10.1155/2023/2237574
Song K, Coleman RA, Zhu X, Alber C, Ballas ZK, Waldschmidt TJ, Cook RT. Chronic ethanol consumption by mice results in activated splenic T cells. J Leukoc Biol. 2002;72(6):1109–16.
D’Souza El-Guindy NB, Kovacs EJ, De Witte P, Spies C, Littleton JM, de Villiers WJS, et al. Laboratory models available to study alcohol-induced organ damage and immune variations: choosing the appropriate model. Alcohol Clin Exp Res. 2010;34(9):1489–511. https://doi.org/10.1111/j.1530-0277.2010.01234.x
Collins MA, Neafsey EJ. Alcohol, Excitotoxicity and Adult Brain Damage: an Experimentally Unproven Chain-of-Events. Front Mol Neurosci. 2016;9:8. https://doi.org/10.3389/fnmol.2016.00008
Motuziuk O, Nozdrenko D, Prylutska S, Vareniuk I, Bogutska K, Braniuk S, et al. The effect of C60 fullerene on the mechanokinetics of muscle gastrocnemius contraction in chronically alcoholized rats. Heliyon. 2023;9(8):e18745. https://doi.org/10.1016/j.heliyon.2023.e18745
Gharbi N, Pressac M, Hadchouel M, Szwarc H, Wilson SR, Moussa F. [60]fullerene is a powerful antioxidant in vivo with no acute or subacute toxicity. Nano Lett. 2005;5:2578–85. https://doi.org/10.1021/nl051866b
Nozdrenko DN, Berehovyi SM, Nikitina NS, Stepanova LI, Beregova TV, Ostapchenko LI. The influence of complex drug cocarnit on the nerve conduction velocity in nerve tibialis of rats with diabetic polyneuropathy. Biomedical Research. 2018;29(19):3629–34. https://doi.org/10.4066/BIOMEDICALRESEACH.29-18-1055
Jung MK, Callaci JJ, Lauing KL, Otis JS, Radek KA, Jones MK, Kovacs EJ. Alcohol exposure and mechanisms of tissue injury and repair. Alcohol Clinical Exp Res. 2011;35(3):392–9. https://doi.org/10.1111/j.1530-0277.2010.01356.x
Monte SM, Kril JJ. Human alcohol-related neuropathology. Acta Neuropathol. 2014;127(1):71–90. https://doi.org/10.1007/s00401-013-1233-3
Melnyk MI, Ivanova IV, Dryn DO, Prylutskyy YuI, Hurmach VV, Platonov M, et al. C60 fullerenes selectively inhibit BKCa but not Kv channels in pulmonary artery smooth muscle cells. Nanomedicine NBM. 2019;19:1–11. https://doi.org/10.1016/j.nano.2019.03.018
Prilutski YuI, Durov SS, Yashchuk VN, Ogul’chansky TYu, Pogorelov VE, Astashkin YuA, et al. Theoretical predictions and experimental studies of self-organization C60 nanoparticles in water solution and on the support. Eur Phys J D. 1999;9(1–4):341–3. https://doi.org/10.1007/s100530050452
Kostyukov AI, Tomiak T. The force generation in a two-joint arm model: Analysis of the joint torques in the working space. Front Neurorobot. 2018;12:77. https://doi.org/10.3389/fnbot.2018.00077
Schukit MA. Ethanol and methanol. In: Goodman & Gillman’s Pharmacological Basis of Therapeutics. 12th ed. Brunton LL ed. New Delhi: MaGraw Hill. 2011:629–47. Available from: https://books.google.com.ua/books?id=e_yAOpyyaowC
Preedy VR, Patel VB, Reilly ME, Richardson PJ, Falkous G, Mantle D. Oxidants, antioxidants and alcohol: implications for skeletal and cardiac muscle. Front Biosci. 1999;4(5): 58–66. https://doi.org/10.2741/A480
Preedy VR, Adachi J, Asano M, Koll M, Mantle D, Niemela O, et al. Free radicals in alcoholic myopathy: indices of damage and preventive studies. Free Radical Biol Med. 2002;32(8):683–7. https://doi.org/10.1016/s0891-5849(01)00794-8
Otis JS, Guidot DM. Procysteine increases alcohol-depleted glutathione stores in rat plantaris following a period of abstinence. Alcohol Alcoholism. 2010;45(6):495–500. https://doi.org/10.1093/alcalc/agq066
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