Absorption and fluorescent properties of potential fluorescent probes of nucleic acids molecular hybridisation, based on phenazine derivatives
Keywords:
phenazines, spectroscopy, polarized fluorescence, fluorescent probe
Abstract
Absorption and fluorescent spectra of imidazo-[4,5-d]-phenazine and its three novel derivatives were studied in ethylacetate and glycerole. Absorption spectra of these derivatives in visible region consist of two bands attributed to different ππ* electronic transitions. Energy values of S1<—S0 electronic transition are calculated for all derivatives. Fluorescence polarization degrees of 2-trifluoridemethylimidazo-[4,5-d]-phenazine and 1,2,3-triazole-[4,5-d]-phenazine are several times higher of that for unsubstituted imidazophenazine, that makes perspective their using as fluorescent probes upon studies of nucleic acid molecular hybridization.
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References
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Zarytova VF, Kutiavin IV. Bioorganicheskaia khimiia.1986;12(7):911-20. (in Russian)
Lokhov SG, Podyminogin MA. Bioconjugate Chem. 1992;3(5):414-9.
Zozulya V, Blagoi Yu, Lober G, Voloshin I. Biophys. Chem. 1997;65:55-63.
Blagoi Yu, Zozulya V, Voloshin I. Biopolimery i kletka. 1997;13(1):22-29. (in Russian)
Zozulya V, Shcherbakova A, Dubey I. J. Fluoresc. 2000;10(1):49-53.
VanAllan JA, Reynolds GA, Adel RE. J. Org. Chem. 1962;27:1659-64.
Temnikova TI. Kurs teoreticheskikh osnov organicheskoi khimii. L:GNTIKhL; 1962. 948p. (in Russian)
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Published
2018-06-25
Cited
How to Cite
Ryazanova, O. A., Zozulya, V. N., Voloshin, L. M., & Karachevtsev, V. A. (2018). Absorption and fluorescent properties of potential fluorescent probes of nucleic acids molecular hybridisation, based on phenazine derivatives. Biophysical Bulletin, 1(12), 49-52. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/12308
Section
Molecular biophysics
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