Na-DNA films with ions of metals
Abstract
The structures of films received by evaporation from solutions of Na-DNA of calf thymus with a concentration of 0.2 mg/ml, containing salts Li, K, Na, Cs, Mg, Zn, Ca are analyzed at various levels of relative humidity and temperatures. The solution of the salt of Na-DNA in a volume of 0.5 ml was poured into a glass quartz cell placed in a hermetically closed vessel with inputs for air pumping. The cell has an area of 420 mm" and the height of lateral walls of 1 mm. The error of stabilization of temperature in the thermostat came to 0.5°C, the error of humidity was 2%. The research was carried out at temperatures of 30 and 40°C. The received films of Na-DNA were photographed in white polarized light. The volume of the formed liquid crystal phase depends on the type of ions in Na-DNA solutions and defines the correlation between the area of structure on the film and the area of all the film and depends on the correlation of concentrations of salt of metal and DNA. The area occupied by liquid crystal structures was calculated as the area limited to a curve, connecting boundary elements of structures. The relative areas of structures received from solutions containing a salt of NaCl with concentrations of 20 and 1.25 mM have values of 0.73 and 0.09 accordingly. The films containing a salt of MgCl2 has spherulite structures collapsing under the conditions of room temperature and humidity. The films received from the solutions containing salt LiCl, CsCl, CaCl2, or MgCl2 with a concentration of 10 mM do not form structures with the allocated direction of growth of clusters. The films received from the solutions containing salt NaCl with the concentration of 10 mM and salts ZnCl2 or CuCl2 with the concentration of 0.4 mM and 0.2 mM respectively do not form structures with the allocated direction of growth of clusters also. It is assumed, the film can be characterized by a set of values of fractal dimensions; one of the signs of the existence of DNA in native form is the presence of such fractal structures as percolation anisotropic cluster in the received film.
Downloads
References
2. Tarasevich YY. Mechanisms and models of the dehydration self-organization in biological fluids (2004). Uspehi Fiz. Nauk , 7, 779-790.
3. Davidson MW, Strzelecka TE, Rill RL. (1988). Multiple liquid crystal phases of DNA at high concentration. Nature, 331, 457-460.
4. Bouligand Y, Livolant F. (1984). The organisation of cholesteric spherulites. J. Physique, 45, 1899-1923.
5. Evdokimov YM, Skuridin SG, Badaev NS. (1986). The proof of formation of two types of liquid crystal microphases of DNA molecules of low molecular weight in water-salt polyethylene glycol solutions. DAN SSSR , 286, 997-1000.
6. Golo VL, Evdokimov YM, Katz YI, Salyanov VI. (2000). Interaction of segments of nucleic acids owing to modification of a network of hydrogen bond of solvent. ZETF, 118, 959-972.
7. Blagoy YP, Galkin VL, Gladchenko GO, Kornilova SV, Sorokin VA, Shkorbatov AF. (1991). Complexes of metals of nucleic acids in solutions. Kiev: Naukova Dumka.
8. Andronicashvili EL, Esipova NG. (1982). Role of metals in the development of some tumours. Biophysica, 6, 1022-1026.
9. Sirover MA, Loeb LA. Infidelity of DNA (1976). Synthesis in vitro: screening for potential metal mutagens or carcinogens. Science, 7, 2331-2335.
10. Andronicashvili EL. (1987). Malignization and change of some physico-chemical properties of biomacromolecules and supramolecular structures. Biophysica, 5, 782-799.
11. Glibitskiy GМ, Krasnitskaja AA, Gudzenko VI. (2006). Structures of Na-DNA films received from solutions at various values of humidity and temperatures. Biophysical Bulletin, 1, 89-94.
12. Glibitskiy GM, Krasnitskaya AA. (2007). Way of definition of influence of substance on nucleic acid. Ukraine patent № 26115, bull. 14.
13. Frey E, Tauber UC, Schwabl F. (1994). Crosover from isotropic to direct percolation. Phys. Review E, 49, 5058–5072.
14. Smalyukh II, Zribi OV, Butler JC, Lavrentovich OD, Wong1 GCL. (2006). Structure and Dynamics of Liquid Crystalline Pattern Formation in Drying Droplets of DNA. Phys. Rev. Lett., 96, 1778.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
