Dependence of intracellular ice formation probability on oversaturation level in yeast-like fungi saccharomyces cerevisiae
Abstract
Based on the general thermodynamical theory of crystal formation in binary solution there was determined the dependence between over-saturation of the intracellular solution and meantime period after the end of which at this over-saturation during freezing in yeast-like like fungi Saccharomyces cerevisiae intracellular crystallization started. The type of functional dependence of pre-exponential multiplier in the expression for the probability of intracellular ice formation on over-saturation in intracellular solution has been established using the general theory of processes of activation type. Since the deriving of initial expression is based solely on general thermodynamical notions the obtained result is universal and may be applied for the analysis of ice nuclei formation in any binary aqueous solution. Specific properties of the cells under freezing and extracellular aqueous solution herewith may be taken into account by means of the setting of the balanced melting curve for certain extracellular solutions and selection (using a small number of auxiliary experiments) of one constant.
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