Joint action of pharmaceuticals in model lipid membranes: calorimetric effects

Keywords: pharmaceuticals, model lipid membranes, membranotropic action, joint action, differential scanning calorimetry

Abstract

Joint action of a number of pharmaceuticals has been studied in multibilayer model membranes of L-α-dipalmitoylphosphatidylcholine. Shift of the main phase transition temperature of the membrane under the pharmaceuticals introduction (∆Tm) was determined by means of differential scanning calorimetry and used as a basic factor of their membranotropic action (MA). Pairs of pharmaceuticals were selected with various character of lipophylicity and MA; cholesterol was used as the membrane compound with the well-known MA. Revelation and identification of the effects of joint action was performed by comparison of ∆Tm values under separate and joint introduction of the pharmaceuticals. Effects of joint action appear similar for hydrophobic azithromycin and hydrophilic succinylcholine in their combinations both with povidone and with cholesterol. Examination of joint action of an active pharmaceutical intgredient (API) and and an excipient allowed us to establish a certain advantage of the API’s MA in the pairs azithromycin-lactose and azithromycin-dimetylsulfoxide, and additivity of the MA in the pair amixin-hypromelose.

Downloads

Download data is not yet available.

Author Biographies

O. V. Vashchenko, Institute for Scintillation Materials NAS of Ukraine

60 Nauky Ave., 61001 Kharkov, Ukraine

L. V. Budianska, Institute for Scintillation Materials NAS of Ukraine

60 Nauky Ave., 61072 Kharkov, Ukraine

References

Viktorov AP, Peredrij VG, Shcherbak AV. Vzaimodejstvie lekarstv i pishchi. K.: Zdrorov’ja; 1991. 240 p. (In Russian)

Baltkajs YaYa, Fateev VA. Vzaimodejstvie lekarstvenny veshchestv. M.: Meditsina; 1991. 304 p. (In Russian)

Pertsev IM, Bashura GS, Alyushin MT. K voprosu vzaimodeystviya lekarstvennykh i vspomo-gatel'nykh veshchestv. Farmatsiya. 1973;5:67. (In Russian)

Kukes VG. Vzaimodeystviye lekarstvennykh sredstv. Farmakol. i toksikol. 1991;2:82-85. (In Russian)

Orme M. Drug absorption in the gut. Br. J. Anaesth. 1984;56:59-67.

Pinheiro M, Silva AS, Pisco S, Reis S. Interactions of isoniazid with membrane models: Implications for drug mechanism of action. Chemistry and Physics of Lipids. 2014;183:184–90.

Castillo JA, Clapés P, Infante MR, Comas J, Manresa A. Comparative study of the antimicrobial activity of bis(Na-caproyl-L-arginine)-1,3-propanediamine dihydrochloride and chlorhexidine dihydrochloride against Staphylococcus aureus and Escherichia coli. J. of Antimicrobial Chemotherapy. 2006;57:691–8.

Knobloch J, Suhendro DK, Zieleniecki JL, Shapter JG, Köper I. Membrane–drug interactions studied using model membrane systems. Saudi Journal of Biological Sciences. 2015;22(6):714–8.

Koukoulitsa C, Kyrikou I, Demetzos C, Mavromoustakos T. The role of the anticancer drug vinorelbine in lipid bilayers using differential scanning calorimetry and molecular modeling. Chemistry and Physics of Lipids. 2006;144:85–95.

Sadchenko AO, Vashchenko OV, Kasian NA. Correlations between thermostability of multibilayer lipid structure and molecular parameters of guest molecules. Funct. Mater. 2016;23(2):230-5.

Seydel JK, Wiese M. Drug-Membrane Interactions: Analysis, Drug Distribution, Modeling. Wiley-VCH Verlag GmbH & Co. KGaA; 2002. 350 р.

Caruso B, Sánchez JM, García DA, de Paula E, Perillo MA. Probing the combined effect of flunitrazepam and lidocaine on the stability and organization of bilayer lipid membranes. A differential scanning calorimetry and dynamic light scattering study. Cell Biochem. Biophys. 2013;66:461-75.

Vashchenko O, Pashynska V, Kosevich M. Lyotropic mesophase of hydrated phospholipids as model medium for studies of antimicrobial agents activity. Mol. Cryst. Liq. Cryst. 2011;507:155-63.

Krasnikova AO, Vashchenko OV, Kasyan NA. Termodinamicheskiye parametry fazovykh perekhodov model'nykh lipidnykh membran kak marker membranotropnogo deystviya antibiotikov v preparatakh-analogakh. Biophys. Bull. 2014;32(2):27-38. (in Russian)

Binderup ML, Dragsted LO. Combined Actions and Interactions of Chemicals in Mixtures. Schultz; 2003. 158 p.

Acree W, editor. Toxicity and Drug Testing. InTech; 2012. 528 p.

Kasian NA, Pashynska VA, Vashchenko OV, Krasnikova AO, Gömöry A, Kosevich MV et al. Probing of the combined effect of bisquaternary ammonium antimicrobial agents and acetylsalicylic acid on model phospholipid membranes: differential scanning calorimetry and mass spectrometry studies. Mol. Biosyst. 2014;10:3155-62.

Fialkov YuYa, Zhitomirskiy AN, Tarasenko YuA. Fizicheskaya khimiya nevodnykh rastvorov. L: Khimiya; 1973. 376 p. (in Russian)

Ohvo-Rekilä H, Ramstedt B, Leppimäki P, Slotte JP. Cholesterol interactions with phospholipids in membranes. Progress in Lipid Research. 2002;41:66–97.

Fa N, Ronkart S, Schanck A, Deleu M, Gaigneaux A, Goormaghtigh E et al. Effect of the antibiotic azithromycin on thermotropic behavior of DOPC or DPPC bilayers. Chem Phys Lipids. 2006;144(1):108-16.

Cited
How to Cite
Vashchenko, O. V., & Budianska, L. V. (1). Joint action of pharmaceuticals in model lipid membranes: calorimetric effects. Biophysical Bulletin, 2(36), 11-18. https://doi.org/10.26565/2075-3810-2016-36-02
Section
Molecular biophysics