The role of processing in age-related changes of collagen thermo-stability
Keywords:
age, collagen, hydroxylation, oxidative desamination, cross-links, thermo-stability, Gibbs available energy
Abstract
The connection between the degree of proline hydroxylation, of lysine and hydroxylysine ε-NH2-groups in collagen oxidative delamination and thermo-stability of the collagen sub-molecular structures in the skin of Wistar rats during postnatal ontogenesis, was in vitro studied. It was shown that, after an increase in these free ε-NH2-groups in collagen towards the age of 3-months, their content further remained constant while at the same time, the content of COH-groups steadily decreases with age, beginning from 1-month. Due to this, inter-molecular cross-linking in collagenous sub-molecular structures decreases from 1 to 3 months of age and afterward, continuously rises up to the age of 24 months. The content of hydroxyproline in collagen continuously decreases during postnatal ontogenesis. The joint action of both effects leads to a decrease in the thermo-stability of collagenous sub-molecular structures in the skin from 1 to 3 months of age, with its further increase up to the age of 24 months.
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References
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10. Bailey A.Y., Paul R.G. The mechanisms and consequences of the maturation and ageing of collagen // Proc. Indian Acad. Sci. (Chem. Sci.). – 1999. – Vol. 111, № 1. – P. 57 – 69.
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12. Yamauchi M., Masashi М. Post-translational Modifications of Proteins: Lysine Hydroxylation and Cross-linking of collagen // Tools for Functional Proteomics. – Series: Methods in Molecular Biology. – 2008. – Vol. 445. P. 95 – 108.
13. И. Клотц. Энергетика биохимических реакций.- М.: Мир.–1970.– 112 с.
14. Гарбузенко О.Б., Емец Е.Б., Перский Е.Э. Влияние деформации на обмен белков и механические свойства аорты и кожи крыс in vitro // Вестн. пробл. биол. и мед. - 1997. - № 25. - С. 12-18.
15. Бейли Дж. Методы химии белков – М.: Мир, 1965. – 284 с.
16. Дубинина Е.Е. Окислительная модификация белков плазмы крови больных с психиатрическими расстройствами // Вопр. мед. химии. – 2000. – №4. – С. 36 – 47.
17. Утевская Л.А., Перский Е.Э. Простой метод определения суммарного и свободного оксипролина // Вестн. Харьк. ун-та. – 1982. – № 226. – С. 18 – 20.
Cited
How to Cite
El-Ta’lu, A. B., Persky, Y. E., Kot, V. G., Nikitina, N. A., & Goenaga, M. V. (1). The role of processing in age-related changes of collagen thermo-stability. Biophysical Bulletin, 1(24). Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/3869
Section
Molecular biophysics
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