Bacterial lysate of Lactobacillus rhamnosus DV suppresses bioluminescence of Photobacterium phosphoreum
Abstract
Background: Recent studies on the lysate of Lactobacillus rhamnosus DV have demonstrated its antimicrobial and fungicidal activities. Classical microbiological methods for evaluating postbiotics and metabiotics are resource-intensive that need for new rapid and sensitive tests systems to assess their antimicrobial and antifungal properties.
Objectives: The aim of this work was to study the effects of the Lactobacillus rhamnosus DV lysate on the intensity of Photobacterium phosphoreum bioluminescence and to demonstrate the potential use of bacterial bioluminescence to determine the bacteriostatic activity of this postbiotic substance.
Materials and methods: The cell lysates of lactic acid bacterial strain L. rhamnosus DV were investigated, which were included in the Del-Immune V® dietary supplement produced by MirImmunoPharm LLC (Ukraine) in cooperation with Stellar Biotics, LLC (USA). The culture of P. phosphoreum IMV B-7071 was used in this study. Bioluminescence measurements were conducted using a digital photo-registration method. Bioluminescence was analyzed using cross-platform open-source software ImageJ (NIH) in brightness units in RGB color space.
Results: The bioluminescence intensity initially increased with population density during the first day, then faded despite the continued growth of cell density in the bacterial suspensions. The addition of Lactobacillus rhamnosus DV lysate significantly suppressed bioluminescence, reducing it to background levels within 24 hours, with the effect varying by concentration. The peak luminescence in control samples occurred at 18 hours, whereas in lysate-treated samples it shifted to 3–6 hours, depending on the lysate concentration.
Conclusions: The lysate of Lactobacillus rhamnosus DV causes a significant decrease in the growth rate of Photobacterium phosphoreum and strongly suppresses the intensity of their bioluminescence, the magnitude of which depends on the concentration of the lysate. This finding supports the use of bacterial luminescence as an effective way for evaluating the bacteriostatic activity of postbiotic substances against pathogenic and opportunistic microorganisms.
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Copyright (c) 2026 Victor Martynyuk, Yuliya Tseyslyer, Olena Gromozova, Igor Hretskyi, Yurii Penchuk, Liubov Shynkarenko Sichel, Tetyana Falaleeva
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