Application of modified waveguide sensor for dielectric study of red blood cells of patients with discirculatory encephalopathy before and after the therapy
Abstract
The paper describes the EHF dielectrometry method which is used for study of dielectric characteristics
of human red blood cells (RBCs) in the free water dispersion region. This method is based on
measurement of complex reflection coefficient from short-circuit multilayer structure (waveguide sensor)
containing the sample in the single-frequency mode (39.5 GHz). An improved in their accuracy
characteristics waveguide sensor has been used. For a given sensor the problem of finding the complex
permittivity has been solved for the first time and realized by solving the direct and inverse problems of
electrodynamics using numerical methods. The direct problem of finding the complex reflection
coefficient was solved by the finite-difference time-domain (FDTD) method. This method makes it
possible simulating the measuring structures of arbitrary shape containing the sample under study. The
inverse problem is implemented by solving a system of nonlinear equations. The algorithm developed has
been successfully applied by the authors for the sensor calibration, which allowed use in the study of the
dielectric characteristics of the blood. As an example of feasible application of improved sensor the
results of measurement dielectric characteristics of RBCs of patients with discirculatory encephalopathy
before and after therapy are represented. It has been revealed an increasing their complex permittivity,
which indicates a change of water state in the cells in the dynamics of therapy. There was a significant
increase in the complex permittivity, which indicates a change of water state in the cells as a result of
medical treatment. The data obtained demonstrates the opportunity of using EHF dielectrometry while
evaluating the state of the patient’s blood cells during their rehabilitation.
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References
2. Time domain dielectric spectroscopy study of human cells II. Normal and malignant white blood cells / Yulia Polevaya, Irina Ermolina, Michael Schlesinger [et al] // Biochimica et Biophysica Acta. – 1999. – № 1419. - P. 257-271.
3. Kuznecov A.N. KVCh-dijelektrometrija biologicheskih zhidkostej v uslovijah narushennogo vodnogo obmena/ A.N. Kuznecov, I.I. Turkovskij, I.A. Volkova // Biofizika. - 2001. - T.46, vyp. 6. – S. 1122-1126.
4. Bao J.- Zh. Microwave dielectric measurements of erythrocyte suspensions / J. - Zh. Bao, C. C. Davis, M. L. Swicord // Biophysical Journal. – 1994. - Vol. 66. - P. 2173-2180.
5. Dielectric properties of human erythrocytes in normal and carcinogenic state / L. V. Batyuk, S. V. Gatash, O. A. Gorobchenko, O. T. Nikolov // Visn. Hark. Un-tu. Biofizychnyj visnyk. – 2002. – T. 10, vyp.1. – S. 54- 57.
6. Ocinka funkcional'nogo stanu beta-adrenoreceptoriv erytrocytiv ljudyny pry arterial'nij gipertenzii' metodom KVCh dielektrometrii' / O. V. Vysoc'ka, K. A. Arhypova, P. S. Krasov [ta in.] // Vostochno-Evropejskij zhurnal peredovyh tehnologij. – 2009. – T. 6/7, № 42. – S. 17-21.
7. Arkhypova K. A. Monitoring the functional properties of human red blood cells by means of waveguide microwave single-frequency dielectrometry method / K.A. Arkhypova // Telecommunications and Radio Engineering. – 2011. - Vol. 70, № 6. - P. 547-552.
8. Wegoleva T.Ju. Issledovanie molekuljarnyh mehanizmov dejstvija doksorubicina na sistemy vnutrikletochnoj signalizacii / T.Ju. Wegoleva, N.S. Nikitina, E.A. Ruzhel'nik // Biofizika. – 2007. – T. 52, vyp. 1. – S. 84-87.
9. Vlijanie kvercetina na dijelektricheskie harakteristiki komponent krovi insulinorezistentnyh krys / A. V. Adel'janov, T. A. Stenkovaja, O. A. Gorobchenko [i dr.] // Biofizychnyj visnyk. – 2009. – T. 1, vyp. 22. – S. 37-41.
10. Laboratornye metody issledovanija sistemy gemostaza / V. P. Baluda, Z. S. Barkagan, E. D. Gol'dberg [i dr.] – Tomsk, 1980. – 311 s. : il.
11. Krasov P. S. Odnochastotnyj reflektometr na osnove chetyrehzondovoj izmeritel'noj linii / P. S. Krasov // Prikladnaja radiojelektronika. - 2008. - № 2. - S. 188-191.
12. Krasov P. S. Instrument for measuring the complex permittivity of biological objects / P. S. Krasov, K. A. Arkhipova // Telecommunications and Radio Engineering. – 2009. - vol. 68, No. 8. – P. 727-733.
13. Sirenko Ju. K. Modelirovanie i analiz perehodnyh processov v otkrytyh periodicheskih, volnovodnyh i kompaktnyh rezonatorah/ Ju. K. Sirenko. – H. : JeDJeNA, 2003. – 231 s. – ISBN 966-8230-04-3.
14. Kobzar' A. I. Prikladnaja matematicheskaja statistika. Spravochnik dlja inzhenerov i nauchnyh rabotnikov / Kobzar' A. I. — M. : Fizmatlit, 2006. — 816 s.
15. Wegoleva T. Ju. Vybor optimal'nyh uslovij izmerenija dijelektricheskoj pronicaemosti biopolimerov volnovodnym metodom / T. Ju. Wegoleva, L. A. Rud' // Biofizika. – 1984. – № 5. – S. 903-907.
16. Arhipova E. A. KVCh dijelektrometrija sil'nopoglowajuwih sred. Istochniki izluchenija, oblast' vzaimodejstvija / E. A. Arhipova, P. S. Krasov, A. I. Fisun // Zhurnal nano- ta elektronnoi' fizyky. - 2010. – T. 2, № 1. – S. 33-41.
17. Krasov P. S. Measuring cuvette simulation of dielectrometer for permittivity investigation of high-loss substances / P. S. Krasov, K. A. Arkhypova, A. I. Fisun // Journal of Measurement Science and Instrumentation. – 2011. - Vol. 2, № 3. - P. 205-208.
18. Krasov P. S. Cuvette simulation in nonresonator dielectrometer for high-loss substance permittivity measurements / P. S. Krasov, K. A. Arkhypova, A. I. Fisun // Journal of Applied Electromagnetism. – 2011. – Vol. 13, № 3. – P. 9-14.
19. Sirenko Y. K. Modeling and analysis of transient processes in open resonant structures: New methods and techniques / Y. K. Sirenko, S. Ström, N. P. Yashina. - N. Y. : Springer, 2007. - 367 p. – ISBN 0387308784.
20. Chislennoe modelirovanie jelektrodinamicheskih harakteristik/ Ju.K. Sirenko, A.I. Vjaz'mitinova, V.L. Pazynin, K.Ju. Sirenko // Jelektromagnitnye volny i jelektronnye sistemy. - 2007. - № 1. - S. 24-34.
21. Volkov E. A. Chislennye metody: uch. posob. dlja vuzov / Volkov E. A. - 2-e izd., ispr. – M. : Nauka, -1987. – S. 64-68.
22. Kalitkin N. N. Chislennye metody / Kalitkin N. N. – M. : Nauka, 1978. – S. 44-48.
23. Shikin E. V. Krivye i poverhnosti na jekrane komp'jutera. Rukovodstvo po splajnam dlja pol'zovatelej / E. V. Shikin, A. I. Plis – M. : DIALOG–MIFI, 1996. – 240 s. : il. – ISBN 5-86404-080-0.
24. Ellison W. J. Permittivity of pure water, at standard pressure, over the frequency range 0-25 THz and the temperature range 0-100°C / W. J. Ellison // J. Phys. Chem. Ref. Data. – 2007. – № 1. – P. 1-18.
25. Atlas kletok krovi i kostnogo mozga / Pod red. G. I. Kozinca – M. : Triada-H, 1998. – 160 s. – ISBN 5-86021-014-0.
26. Dolgova I. N. O vlijanii arterial'noj gipertenzii na razvitie discirkuljatornoj jencefalopatii / I. N. Dolgova, A. I. Starodubcev // Nevrologicheskij vestnik. – 2010. – T. XLII, № 3. – S. 51-54.
27. Denisov E. N. Izmenenie parametrov cirkulirujuwih jeritrocitov u bol'nyh arterial'noj gipertenziej / E. N. Denisov // Vestnik OGU. – 2006. – № 4. – S. 127–129.
28. Decreased water and potassium content in erythrocytes in essential hypertension / T. Kawarabayashi, Y. Kanayama, K. Takeuchi [et al] // Hypertension. – 1986. – № 8. – P. 618-624.
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