Texture of na-dna films received from solution containing tris and edta
Keywords:
DNA, ion, solution, humidity, liquid crystals, filmAbstract
Influence of Tris and EDTA on the formation of texture of DNA film is examined. The films were
obtained from solutions of calf thymus Na-DNA salts with a concentration of 0.2 mg / ml in 10 mM
solution of NaCl, as well as with different combinations: 10 mM NaCl and 10 mM Tris; 10 mM NaCl and
1 mM EDTA; 10 mM NaCl, 10 mM tris and 1 mM EDTA. Accuracy of temperature stabilization is
0.5 ºC, error of relative humidity determination is 2-3%. Films from solutions of Na-DNA with the
addition of 10 mM NaCl and 10 mM Tris and films obtained from solutions of Na-DNA with the addition
of 10 mM NaCl and 1 mM EDTA do not have the textures on the surface after drying. The films obtained
from the Na-DNA solution (at a concentration of 0.2mg/ml), 10 mM NaCl, 10 mM Tris and 1 mM EDTA
give textures similar to cholesteric spherulite textures, which are fully manifested in 40-50 hours after
removal of films from the camera
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