INTERACTION OF Ni^2+ IONS WITH HOMOPOLYNUCLEOTIDES CONTAINING ADENINE AND URACIL AND THEIR MONOMERS

  • V. A. Sorokin B.I.Verkin Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine, Kharkov
  • V. A. Valeev B.I.Verkin Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine, Kharkov
  • M. V. Degtiar B.I.Verkin Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine, Kharkov
  • G. O. Gladchenko B.I.Verkin Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine, Kharkov
  • Yu. P. Blagoi B.I.Verkin Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine, Kharkov

Abstract

Interactions of Ni2+ ions with AMP, UMP, single-chain poly A, poly U, double-chain poly A poly U and three-chain poly A*2 poly U have been studied by the method of differential UV spectroscopy. The coiL->helix transition observed in polynucleotides is due to the Ni2+ ion binding to oxygen atoms of phosphate groups. The nickel coordination to bases takes place only in the case of single AMPs and single-chain poly A. The formation of a chelate N7-Ni2+-(P04) induces melting of this polymer. Melted parts form compact particles with the effective radius ~100A.

KEY WORDS: UV spectroscopy, homopolynucleotides, metal ions.

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References

1. E.L.Andronikashvili// Biofizika, 1987, V. 23, N 5, p. 782-799.


2. V.A.Sorokin, V.A.Valeev, G.O.Gladchenko, I.V.Sysa, I.V.Volchok, Yu.P.Blagoi// J. Inorg. Biochem., 1996, V. 63, N 2, p. 79-98.

3. K.Aoki, in: Nucleosides, Nucleotides and Metal Ions in Bioactive Molecules. Elsevier: Amsterdam, Oxford, New York, Tokio, 1988, V. 8, Chapt. 13.

4.I.A.Il'icheva, V.L.Florentiev// Mol. Biol., 1992, V. 26, N 3, p. 512-551.

5. V.A.Sorokin// Mol. biol, 1988, V. 22, N 6, p. 1590-1598.

6. V.A.Sorokin. V.A Valeev, G.O.Gladchenko, Yu.P.Blagoi// Biophysics, 1994, V. 39, N 5, p. 821-832.

7. H. Krakauer, J.M.Sturtevant// Biopolymers, 1968, vol. 6, p. 491-512.

8. D.N.Holcomb, S.N.Timasheff// Biopolymers, 1968, vol. 6, N 4, p. 513-529.

9. Yu.P.Blagoi, V.L.Galkin, G.O.Gladchenko, S.V.Kornilova, V.A.Sorokin, A.G.Shkorbatov, Metallokompleksy nukleinovykh kislot v rastvorakh, Kiev, Naukova Dumka, 1991.

10. M. Riley. B.Maling, M.J.Chamberlin// J. Mol. Biol., 1966, V. 20, p. 359-389.

11. V.A.Sorokin. Yu.P.Blagoi. V.A.Valeev, S.A.Khomenko, L.A.Morozova// Mol. Biol., 1982, V. 16, N 2, p. 369-378.

12. A.V.Borodavkin, E.I.Budovskii, Yu.V.Morozov, F.A.Savin, N.A.Simukova, In: Itogi Nauki i Tekhniki. Ser. Mol. Biol.
Vol. 14. Moscow: VTNITI, 1977. .

13. S.-H.Kim, R.B.Martin // Inorg. Chim. Acta, 1984, V. 91, N 1, p. 19-24.

14. R. W.Gellert, R.Bau, R.B.Martin, Ya.H.Mariam, In: Metal Ions in Biological Systems. New York: 1979, V. 8, Chapts 1-2.

15. A.Yamada, K.Akasaka,H.Hatano// Biopolymers, 1976, V. 15, N7, p. 1315-1331.

16. K.Enmanji// J. Polym. Sci., 1987, V. 22, N 3, p. 883-895.

17. I.Ya.Slonim// Optika i Spektroskopiya, 1960, V. 8, N 1, p. 98-108.

18. A.Yu.Grosberg, A.R.Khokhlov, Statisticheskaya Fizika Makromolekul, Moscow: Nauka, 1989. '

19. V.A.Sorokin, Yu.P.Blagoi, V.A.Valeev, Yu.V.Lisnyak// J. Inorg. Biochem., 1983, V. 19, p. 143-153.

20 . H.Krakauer// Biochemistry, 1974, V. 13, N 12, p. 2579-2589.

21. R.M.Clement, J.Sturm, M.Daune// Biopolymers, 1973, V. 12, N 2, p. 405-421.

22. W.Saenger, Printsypy strukturnoi organizatsii nukleinovykh kislot, Moscow: Mir, 1987.

23. R.Lavery, A.Pullman, B.Pullman// Int. J. Quant. Chem., 1981, V. 20, N 1, p. 49-62.
Cited
How to Cite
Sorokin, V. A., Valeev, V. A., Degtiar, M. V., Gladchenko, G. O., & Blagoi, Y. P. (1). INTERACTION OF Ni^2+ IONS WITH HOMOPOLYNUCLEOTIDES CONTAINING ADENINE AND URACIL AND THEIR MONOMERS. Biophysical Bulletin, 3(5). Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/2482
Issue
Vol. 3 No. 5 (1999): Біофізичний вісник
Section
Molecular biophysics

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