The effect of microwave radiation of low intensity on cell viability and chromatin state in human cells
Keywords:
microwave radiation, human cell damage
Abstract
The effects of low intensity microwave radiation on the viability of the epithelial cells human buccal epithelium and chromatin state after in vitro microwave irradiation were investigatted. After the exposure to low-energy microwave radiation (frequency of 36.64 GHz and 1.85 GHz) the cell damage was assessed by staining with vital dye Hoechst 33342 and ethidium bromide . Condensation of chromatin in the cells was determined by the change in the number of granules of heterochromatin after staining with orcein in interphase nuclei. The cell damage after microwave irradiation was partially recovered after a certain period of time (from 1.5 to 4 hours) in dependence on radiation intensity. The low -energy microwave radiation also induced an increase in chromatin condensation (heterochromatinization) independence on the duration of cell exposure to microwavesDownloads
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References
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10. Shckorbatov Y. G. He-Ne laser light induced changes in the state of the chromatin in human cells / Y. G. Shckorbatov // Naturwissenschaften. – 1999. – V. 86(9). – P. 450–453.
11. On age-related changes of cell membrane permeability in human buccal epithelium cells / Y. G. Shckorbatov, V. G. Shakhbazov, A. M. Bogoslavsky, A. O. Rudenko // Mech. Ageing Develop. – 1995. – V. 83. – P.87–90.
12. Cell phones and brain tumors: a review including the long-term epidemiologic data / V. G. Khurana, C. Teo, M. Kundi [et al.] // Surg. Neurol. – 2009 – V. 72. – P. 205–215.
13. Cellular phone use and risk of benign and malignant parotid gland tumors – a nationwide case-control study / S. Sadetzki, A. Chetrit, A. Jarus-Hakak [et al.] // Am. J. Epidemiol. – 2008. – V. 167, (4). – P. 457–467.
14. O’Keefe S. Does the use of cell phones cause brain tumors? / S. O’Keefe // Clin. J. Oncol. Nurs. – 2008. – V. 12, № 4. – P.671–672.
15. Brain tumour risk in relation to mobile telephone use: results of the INTERPHONE international case-control study / E. Cardis, I. Deltour, M. Vrijheid [et al.] // Int. J. Epidemiol. – 2010. – V. 39. – P. 675–694.
16. Lewin B. Genes VIII / B. Lewin. – New York: Pearson Prentice Hall. – 2004. – 1002 p.
17. Shckorbatov Yu. The state of chromatin as an integrative indicator of cell stress / Yu. Shckorbatov. In: New Developments in Chromatin Research // N.M. Simpson V.J. Stewart [Ed.]. – New York: Nova Publishers. – 2012. – P. 123–144.
Cited
How to Cite
Shkorbatov, Y. G., Katrych, V. A., Pasyuga, V. N., Antonenko, E. A., Ivanchenko, D. D., Klyuchivskaya, O. Y., & Stoika, R. S. (1). The effect of microwave radiation of low intensity on cell viability and chromatin state in human cells. Biophysical Bulletin, 1(29). Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/2369
Section
Action of physical agents on biological objects
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