The effect of microwave radiation of low intensity on cell viability and chromatin state in human cells

  • Y. G. Shkorbatov V.N.Karazin Kharkov National University, Institute of Biology
  • V. A. Katrych V.N.Karazin Kharkov National University, Department of Physical and Biomedical Electronics and Complex Information Technologies
  • V. N. Pasyuga V.N.Karazin Kharkov National University, Institute of Biology
  • E. A. Antonenko V.N.Karazin Kharkov National University, Department of Physical and Biomedical Electronics and Complex Information Technologies
  • D. D. Ivanchenko V.N.Karazin Kharkov National University , Department of Theoretical Radiophysic
  • O. Y. Klyuchivskaya Institute of Cell Biology, National Academy of Sciences of Ukraine
  • R. S. Stoika Institute of Cell Biology, National Academy of Sciences of Ukraine
Keywords: microwave radiation, human cell damage

Abstract

The effects of low intensity microwave radiation on the viability of the epithelial cells human buccal epithelium and chromatin state after in vitro microwave irradiation were investigatted. After the exposure to low-energy microwave radiation (frequency of 36.64 GHz and 1.85 GHz) the cell damage was assessed by staining with vital dye Hoechst 33342 and ethidium bromide . Condensation of chromatin in the cells was determined by the change in the number of granules of heterochromatin after staining with orcein in interphase nuclei. The cell damage after microwave irradiation was partially recovered after a certain period of time (from 1.5 to 4 hours) in dependence on radiation intensity. The low -energy microwave radiation also induced an increase in chromatin condensation (heterochromatinization) independence on the duration of cell exposure to microwaves

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Author Biographies

Y. G. Shkorbatov, V.N.Karazin Kharkov National University, Institute of Biology
61022, pl. Svobody, 4,Ukraine
V. A. Katrych, V.N.Karazin Kharkov National University, Department of Physical and Biomedical Electronics and Complex Information Technologies

Kharkov, 61022, pl. Svobody, 4, Ukraine

V. N. Pasyuga, V.N.Karazin Kharkov National University, Institute of Biology

 Kharkov, 61022, pl. Svobody, 4, Ukraine

E. A. Antonenko, V.N.Karazin Kharkov National University, Department of Physical and Biomedical Electronics and Complex Information Technologies

Kharkov, 61022, pl. Svobody, 4, Ukraine

D. D. Ivanchenko, V.N.Karazin Kharkov National University , Department of Theoretical Radiophysic

Kharkov, 61022, pl. Svobody, 4,Ukraine

O. Y. Klyuchivskaya, Institute of Cell Biology, National Academy of Sciences of Ukraine

Lviv, 79005, ul. Drahomanova 14/16

R. S. Stoika, Institute of Cell Biology, National Academy of Sciences of Ukraine

Lviv, 79005, ul. Drahomanova 14/16

References

1. Subjective symptoms, sleeping problems, and cognitive performance in subjects living near mobile phone base stations / H.-P. Hutter, H. Moshammer, P. Wallner, M. Kundi //Occup. Environ. Med. – 2006 – V. 63. – P. 307–313.

2. Hardell L. Epidemiological evidence for an association between use of wireless phones and tumor diseases / L. Hardell, M. Carlberg, Mild K. Hansson // Pathophysiology. – 2009. – V. 16 – P. 113–122.

3. Living near overhead high voltage transmission power lines as a risk factor for childhood acute lymphoblastic leukemia: a case-control study / M.-R. Sohrabi, T. Tarjoman, A. Abadi, P. Yavari // Asian Pacific Journal of Cancer Prevention. – 2010. – V. 11. – P. 423–427.

4. Non-thermal effects of 2.45 GHz microwaves on spindle assembly, mitotic cells and viability of Chinese hamster V-79 cells / M. Ballardin, I. Tusa, N. Fontana [et al.] // Mutat. Res. – 2011. – V. 716. (1-2). – P. 1–9.

5. Non-thermal cellular effects of lowpower microwave radiation on the lens and lens epithelial cells / Y. Yu, K. Yao // J. Int. Med. Res. – 2010 – V. 38(3). – P.729–36.

6. Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts / Y. G. Shckorbatov, V. N. Pasiuga, E. I. Goncharuk [et al.] // J. Zhejiang Univ. Sci. B. – 2010. – V. 11 (10). – P. 801–805.

7. Cell nucleus and membrane recovery after exposure to microwaves / Y. G. Shckorbatov, V. N. Pasiuga, N. N. Kolchigin [et al.] // Proc. Lat. Acad. Sci. Sect. B. – 2011. – V. 65 (672/673). – P. 13–20.

8. Влияние циркулярно-поляризованного низкоинтенсивного излучения миллиметрового диапазона на проницаемость клеточной мембраны в клетках человека / В. Н. Пасюга, Ю. Г. Шкорбатов, В. А. Грабина [и др.] // Біофізичний вісник. – 2008. – № 20 (1). – С. 107–113. /Vlijanie cirkuljarno-poljarizovannogo nizkointensivnogo izluchenija millimetrovogo diapazona na pronicaemost' kletochnoj membrany v kletkah cheloveka / V. N. Pasjuga, Ju. G. Shkorbatov, V. A. Grabina, [i dr.] // Bіofіzichnij vіsnik. – 2008. – № 20 (1). – S. 107–113./

9. The influence of differently polarized microwave radiation on chromatin in human cells / Y. G. Shckorbatov, V. N. Pasiuga, N. N. Kolchigin [et al.] // International Journal of Radiation Biology. – 2009. – V. 85(4). – P. 322–329.

10. Shckorbatov Y. G. He-Ne laser light induced changes in the state of the chromatin in human cells / Y. G. Shckorbatov // Naturwissenschaften. – 1999. – V. 86(9). – P. 450–453.

11. On age-related changes of cell membrane permeability in human buccal epithelium cells / Y. G. Shckorbatov, V. G. Shakhbazov, A. M. Bogoslavsky, A. O. Rudenko // Mech. Ageing Develop. – 1995. – V. 83. – P.87–90.

12. Cell phones and brain tumors: a review including the long-term epidemiologic data / V. G. Khurana, C. Teo, M. Kundi [et al.] // Surg. Neurol. – 2009 – V. 72. – P. 205–215.

13. Cellular phone use and risk of benign and malignant parotid gland tumors – a nationwide case-control study / S. Sadetzki, A. Chetrit, A. Jarus-Hakak [et al.] // Am. J. Epidemiol. – 2008. – V. 167, (4). – P. 457–467.

14. O’Keefe S. Does the use of cell phones cause brain tumors? / S. O’Keefe // Clin. J. Oncol. Nurs. – 2008. – V. 12, № 4. – P.671–672.

15. Brain tumour risk in relation to mobile telephone use: results of the INTERPHONE international case-control study / E. Cardis, I. Deltour, M. Vrijheid [et al.] // Int. J. Epidemiol. – 2010. – V. 39. – P. 675–694.

16. Lewin B. Genes VIII / B. Lewin. – New York: Pearson Prentice Hall. – 2004. – 1002 p.

17. Shckorbatov Yu. The state of chromatin as an integrative indicator of cell stress / Yu. Shckorbatov. In: New Developments in Chromatin Research // N.M. Simpson V.J. Stewart [Ed.]. – New York: Nova Publishers. – 2012. – P. 123–144.
Cited
How to Cite
Shkorbatov, Y. G., Katrych, V. A., Pasyuga, V. N., Antonenko, E. A., Ivanchenko, D. D., Klyuchivskaya, O. Y., & Stoika, R. S. (1). The effect of microwave radiation of low intensity on cell viability and chromatin state in human cells. Biophysical Bulletin, 1(29). Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/2369
Section
Action of physical agents on biological objects