Measurement of the permeability of plasma membrane of murine bone marrow cells to water and cryoprotectants
Keywords:
murine bone marrow cells, osmotic behaviour, Kedem Katchalsky formalism, permeability parameters, dimethyl sulfoxide, propylene glycol, glycerol
Abstract
The authors studied the osmotic behavior of murine bone marrow cells in the hypertonic solutions of permeating cryoprotectants dimethyl sulfoxide (Me2SO), propylene glycol, and glycerol. Using the Kedem Katchalsky formalism the permeability parameters of the plasma membrane for water and mentioned cryoprotectants were determined. The results demonstrate a hydraulic conductivity, Lp in the presence of cryoprotectant of 1.00 ± 0.09, 1.07 ± 0.12, and 1.19 ± 0.15 (x 10 -13 m3/s/N) and cryoprotectant permeability coefficient. K1, of 6.92 ± 0.08. 11.83±0.11 and 0.94 ±0.13 (x 10 -8 m/s) for Me2SO, propylene glycol and glycerol, respectively.
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References
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2. Gavrilov O.K., Kozinets G. I., Chernyak N.B. Cells of the bone marrow and the peripheral blood. Moscow. "Meditsina”. 1985. 288 p.
3. Blinks J.R. // J. Physiol. 1965. 177. P. 42-57.
4. Hepling H.G., Thompson D. and Dupre A. // J. Cell Physiol. 1977. P. 293-302.
5. Liu J., Zieger M. A.J., Lakey J.R.T. et al. //Cryobiology. 1997. P. 1-13.
6. McGrath J. //Cryobiology. 1997. P. 315-334
7. Chaveiro A.E.N., Liu J., Woelders H. and Critser J.K. // Proceedings of the 38th Meeting of the Society of Cryobiology. University of Edinburgh. 2001. P. 170
8. Woods E.J., Liu J., Gilmore J.A. et al // Cryobiology. 1999. 38. P. 200-208.
Published
2002-06-06
Cited
How to Cite
Kholodnyy, V. (2002). Measurement of the permeability of plasma membrane of murine bone marrow cells to water and cryoprotectants. Biophysical Bulletin, 1(10), 73-77. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/18418
Section
Cell biophysics
Copyright (c) 2002 Biophysical visnik
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