Effects of the adrenaline on the transmembrane potential of the single hepatocytes by fluorescent probes
Abstract
The absolute values of plasmatic potential, dynamics of transmembrane potential changes in response to adrenaline have been determined in isolated rats hepatocytes by microfluorimetry of single cells stained with fluorescent probe H 510 (3,3-diethiloxacarbocyanine bromide). To evaluate the potential values the Nernst formula was adapted for the cationic fluorescent probes. The obtained data show that the adrenaline provokes phase changes of the potential (phase of depolarization and phase of hyperpolarization). Microfluorimetry of single cells has revealed that the hyperpolarization is observed at the first minutes of the hormone stimulation. Along with general tendency, the individual responses of some cells to hormone stimulation have been revealed.
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References
Bortner CD, Gomez-Angelats M, Cidlowski JA. Plasma membrane depolarization without repolarization is
an early molecular event in apoptosis mediated by the inactivation of the Na+
/K+ - ATPase//J. Biol. Chem.2001. 276:4304-4314.
Deckers CL, Lyons AB, Samuel K, Sanderson A, Maddy AH. Alternative pathways of apoptosis induced
by methylprednisolone and valinomycin analyzed by flow cytometry//Exp Cell Res. 1993. 208: 342-370.
Mann C, Cidlowski JA. Glucocorticoids regulate the plasma membrane potential of primary thymocytes in
vivo and in vitro //Endocrinology 2001. 142: 421-429.
Freedman J.C., Laris P.C. Electrophysiology of cells and organelles: studies with optical potentiometric
indicftjrs//Intern. Rev. Cytol. 1981. 12.:177-246.
Добрецов Г.Е. Флуресцентные зонды в исследовании клеток, мембран и липопротеинов.-М.:
Наука1989.-277с.
Канаева И.П., Карякин А.В., Аленичева Т.В., Бурмакова Г.А., Алимов Г.А., Арчанов А.М., Зинкович
Г.Д. // Цитология. 1975. Т. 17. с. 545-551.
Ehrenberg B, Montana V, Wei M., Wuskell JP, Loew LM. Membrane potential can be determined in
individual cells from the Nernstian distribution of cationic dyes//Biophys. J. 1988.53. 785-794.
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