Modeling of heat absorption curves for ligand-competitor-DNA triple system
Abstract
The model for a ligand-competitor-DNA triple system is suggested. The model is based on chemical equilibria equations and allows one to analyze DSC experimental data. It is shown that the competitor addition alters the percentage of ligand bound to DNA both at room temperatures and at temperatures close to the melting temperature of DNA. Given the same binding constants and binding sites determined at room temperatures, both qualitative and quantitative differences in calorimetric melting curves can be detected depending on the enthalpy of binding.
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Edenharde R. Antimutagenic effects and possible mechanisms of action of vitamins and related compounds against genotoxic heterocyclic amines from cooked food / R. Edenharde, A. Worf-Wandelbur , M. Deker, K.L. Platt // Mutation Research. – 1999. – V.444. – P. 235–248.
Usha S. Modulation of DNA intercalation by resveratrol and genistein/ S. Usha, I.M. Johnson, R. Malathi // Molecular and Cellular Biochemistry. – 2006 . – V. 284. – P. 57–64.
Kimura H. Decrease in sensitivity to ethidium bromide by caffeine, dimethylsulfoxide or 3-aminobenzamide due to reduced permeability / H. Kimura // J. Pharmacobiodyn. – 1989. – N.12. – P.589–595.
Traganos F. Caffeine modulates the effects of DNA-intercalating drug in vitro: A flow cytometric and spectrophotometric analysis of caffeine interaction with novatrone, doxorubicin, ellipticine and doxorubicin analogue ADI98 / F. Traganos , J. Kapuscinski , Z. Darzynkiewicz // Cancer Res. – 1991. – V. 51. – P. 3682– 3689.
Kapuscinski J. The modulation of the DNA-damaging effect of polycyclic aromatic agents by xanthines: Part I. Reduction of cytostatic effects of quinacrine mustard by caffeine / J. Kapuscinskia, B. Ardelt, J. Piosik, M. Zdunek, Z. Darzynkiewicz // Biochemical Pharmacology. – 2002. – V. 63. №. 4. – P. 625–634.
Piosik J. The modulation by xanthines of the DNA-damaging effect of polycyclic aromatic agents Part II. The stacking complexes of caffeine with doxorubicin and mitoxantrone / J. Piosik, M. Zdunek, J. Kapuscinski // Biochemical Pharmacology. – 2002. – V. 63. – P. 635–646.
Evstigneev M. P. 1H NMR study of the hetero-association of flavin-mononucleotide with mutagenic dyes: ethidium bromide and proflavine / M. P. Evstigneev, Yu. V. Mukhina, D. B. Davies // Molecular Physics. - 2006. – V. 104. №. 4. – P. 647–654.
Davies D. B. Hetero-association of caffeine and aromatic drugs and their competitive binding with a DNA oligomer / D. B. Davies, D. A. Veselkov, L. N. Djimant, A. N. Veselkov // Eur. J. Biophys. – 2001. – V.30. – P.354–366. 58 Е.В. Духопельников
Baranovsky S. F. Interaction of ethidium bromide and caffeine with DNA in aqueous solution / S. F. Baranovsky, P. A. Bolotin, M. P. Evstigneev, D. N. Chernyshev // J. of Appl. Spectroscopy. – 2009. – V. 76., № 1. – P. 143–150.
Davies D. B. Binding of anthracycline antibiotic with DNA in the presence of aromatic vitamin / D. B. Davies, M. P. Evstigneev, Yu. V. Mukhina // Biophys. Chem. – 2005. – V.50. – P.153–163.
Piosik J. De-intercalation of ethidium bromide and propidium iodine from DNA in the presence of caffeine. / J. Piosik, K. Wasielewski, A. Woziwodzka, W. Śledź, A. Gwizdek-Wiśniewska // Cent. Eur. J. Biol. – 2010. – V. 5., № 1. – P. 59–66.
Pietrzak M. Attenuation of acridine mutagen ICR-191 – DNA interactions and DNA damage by the mutagen interceptor chlorophyllin / M. Pietrzak, H. D. Halicka, Z. Wieczorek, J. Wieczorek, Z. Darzynkiewicz. // Biophys. Chem. – 2008. – V.135. -№ 3. – P.69–75.
Pietrzak M. The “interceptor” properties of chlorophyllin measured within the three-component system: Intercalator-DNA-chlorophyllin / M. Pietrzak, Z. Wieczorek, J. Wieczorek, Z. Darzynkiewicz // Biophysical Chemistry. – 2006. – V.123. – P.11–19.
Iermak Ie. Light-absorption spectroscopy of mutagen-DNA complex in presence of competing biologically active compounds / Ie. Iermak, A. Woziwodzka, A. Gwizdek-Wisniewska, J. Piosik // General Assembly and Scientific Symposium, 2011 XXXth URSI. – Р. 1– 4.
Dukhopelnikov E. V. Determination of ligand to DNA binding parameters from two-dimensional DSC curves / E. V. Dukhopelnikov, E. G. Bereznyak, A. S. Khrebtova, A. O. Lantushenko, A. V. Zinchenko // J Therm Anal Calorim . – 2013. – V. 111. – P. 1817–1827.
McGhee J. D. Theoretical calculations of the helix–coil transition of DNA in the presence of large, cooperatively binding ligands / J. D. McGhee // Biopolymers. – 1976. – V. 15 I., № 7. – P. 1345–1375.
Teif V. B. Statistical-mechanical lattice models for protein-DNA binding in chromatin / V. B. Teif, K. Rippe // Journal of Physics: Condensed Matter.. – 2010. – V. 22., №. 41. – P. 414105–414118.
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