Human erythrocytes resistance to haemolysis caused by polypeptide antibiotic gramicidin S

E. V. Hackl University of Manchester, Institute of Science and Technology, Manchester M60 1QD
V. P. Berest V.N. Karazin Kharkiv National University
S. V. Gatash V.N. Karazin Kharkiv National University

Keywords: erythrocyte, gramicidin S, haemolysis, temperature, turbidimetry

Abstract

Lytic activity of gramicidin S underlies its antimicrobial potential and at the same time restricts system clinical application of this polypeptide antibiotic. GS synthetic analogs are aimed at dissociating its antibacterial and hemolytic activity in a way that to dramatically diminishes the later effect. Structure-function relations in a rigid planar cyclic decapeptide molecule allow goal-seeking new preparations. Another key bit for a successful drug discovery policy is a clear understanding of the mechanism of GS interaction with cellular membranes. In the present paper with the help of turbidimetric technique the temperature and concentration dependence of GS-induced hemolysis has been studied. The activation energy of the human red blood cells hemolysis under the action of GS was estimated. It was shown that the analysis of the GS-induced hemolysis data allows gathering additional information on the state of membrane lipid bilayer if compared to conventional HCl-induced hemolysis.

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Author Biographies

E. V. Hackl, University of Manchester, Institute of Science and Technology, Manchester M60 1QD

UK

V. P. Berest, V.N. Karazin Kharkiv National University

4 Svobody Sq., Kharkiv 61077, Ukraine

S. V. Gatash, V.N. Karazin Kharkiv National University

4 Svobody Sq., Kharkiv 61077, Ukraine

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