Self-association and hydration of flavin-mononucleotide in solutions and films
Abstract
In this paper a comparative study of self-association and hydration of flavin-mononucleotide (FMN) in neutral and weakly acidic solutions, and in films grown from solutions with different pH (7 and 6,2), was carried out using IR spectroscopy and piezogravimetry methods. The IR spectra were obtained in the absorption region of intraring and carbonyl vibrations, which are sensitive to the formation of stacking associates. There was detected a high frequency shift of the absorption bands of the C2=O and C4=O carbonyl groups of FMN isoalloxazine rings in films grown from weakly acidic solutions (pH=6,2); this shift is attributed to the dynamic interaction of transition moments of the carbonyl groups. On the basis of theoretical calculations in the dipole-dipole approximation a n-mer model of FMN self-associates wasbuilt. It is shown that the stacking-associates of FMN in films are stabilized by interplanar interactions and the aqueous environment.
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