Comparative analysis of the mechanisms of divalent metals' cations interaction with the Na+/Ca2+ and Ca2+/H+-exchangers of liver mitochondria

  • N. V. Nalyvajko Ivan Franko National University of Lviv
  • L. S. Vovkanych Ivan Franko National University of Lviv
  • L. O. Dubitsky Ivan Franko National University of Lviv
Keywords: mitochondria, calcium, calcium-proton exchanger, calcium-sodium exchanger, metals` cations

Abstract

The mechanisms of divalent metals' cations interaction with the systems of Na+- and H+-dependent Ca2+ efflux from liver mitochondria has been studied. The inhibition of Na+- and H+-dependent Ca2+ efflux from mitochondria by divalent metals` cations has been proved. Kinetic analysis reveals the mainly competitive inhibition of Na+ and H+-dependent Ca2+ efflux from mitochondria by metals` cations. Potency of inhibition in both cases increases in the same order: Ba2+ < Mg2+ < Sr2+ < Mn2+. The results of correlation analysis shows that power of inhibition of the Na+- and H+-dependent Ca2+ efflux from mitochondria by divalent metals` cations depends on their physical-chemical parameters, especially on their crystallographic radius, hydration enthalpy, stability constants of metal cations complexes with oxygen-containing ligands, ionization potential, etc. The conclusion is made that Ca2+ ions translocation by the mitochondrial exchangers and its inhibition by the cations of divalent metals are connected with their interaction with the oxygen-containing ligands of the Ca2+-transporting systems and are accompanied with the ions reversible hydration-dehydration and unaccompanied cooperative interaction during banding its with this systems` ion-transporting centers.

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Author Biographies

N. V. Nalyvajko, Ivan Franko National University of Lviv

 Grushevskogo str. 4, Lviv 79005, Ukraine

L. S. Vovkanych, Ivan Franko National University of Lviv

Grushevskogo str. 4, Lviv 79005, Ukraine

L. O. Dubitsky, Ivan Franko National University of Lviv

Grushevskogo str. 4, Lviv 79005, Ukraine

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Published
2006-06-06
Cited
How to Cite
Nalyvajko, N. V., Vovkanych, L. S., & Dubitsky, L. O. (2006). Comparative analysis of the mechanisms of divalent metals’ cations interaction with the Na+/Ca2+ and Ca2+/H+-exchangers of liver mitochondria. Biophysical Bulletin, 1(17), 48-53. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/12850
Issue
Vol. 1 No. 17 (2006): Біофізичний вісник
Section
Cell biophysics
Copyright (c) 2006 Biophysical visnik

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