The study of the ozone influence on bovine serum albumin and cholinesterase by the optical spectroscopy methods
Keywords:
ozone, bovine serum albumin, cholinesterase, fluorescence, conformations changes
Abstract
From the experimental data submitted in clause follows, that action of ozone on biopolymers was accompanied by change of their spectral characteristics that testifies about conformations reorganizations and inactivation or stimulations of ferment activity. A degree of structural changes and ferment activity of biopolymers depend on concentration of ozone in environment of incubation. The handling by ozone small doses called a raise of cholinesterase activity, whereas the higher doses called ferment inactivation. The given property of ozone, probably, it is possible to apply as reactivate at reconservation a biological material after freezing.
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References
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Gryzunov IuA, Dobretcova GE, editors. Albumin syvorotki krovi v klinicheskoi meditcine. Moskva:GEOTAR; 1998. 440p. (in Russian)
Demchenko AP. Ultrafioletovaia spektrofotomtriia i struktura belkov. Kiev:Nauk. dumka; 1981. 208p. (in Russian)
Morozova TF, Romodanova EA, Diubko TS, Timchenko NN. Spektroskopicheskoe issledovanie vliianiia zamorazhivaniia na plazmu kordovoi krovi. Biofizicheskii vestnik. 2002;2(11):110-5. (in Russian)
Dobretcov GE. Fluorestcentnye zondy v issledovanii kletok, membran i lipoproteinov. M.:Nauka; 1989. 277p. (in Russian)
Menshikov VV. Laboratornye metody issledovaniia v klinike. M.:Meditcina; 1987. 364p. (in Russian)
Razumovskii SD, Zaikov GE. Ozon i ego reaktcii s organicheskimi soedineniiami. M.:Nauka; 1974. 322p. (in Russian)
Grek AM, Lugovoi VN, Bіlous AM. Vpliv krіoprotektorіv na spektri fluorestcentcії albumіnu sirovatki krovі bika. Ukraїnskii bіokhіmіchnii zhurnal. 1974;46:62-6. (in Ukrainian)
Burshtejn JeA. Sobstvennaja ljuminescencija belkov. Biofizika. M.:VINITI AN SSSR; 1997. 189p. (in Russian)
Demchenko AP. Ljuminescencija i dinamika struktury belkov. Kiev:Nauk.dumka; 1988. 277p. (in Russian)
Sorkina DA, Zelevskaja IN. Strukturno-funkcional'nye svojstva belkov:Uchebnoe posobie. K.:Vishha Shkola; 1990. 216p. (in Russian)
Lakowicz JR. Principles of Fluorescence Spectroscopy. New York, Boston, Dordrecht, London, Moscow:Kluwer Academic/Plenum Publishers; 1999. 698p.
Dyubko TS, Nardid OA, Okladnoy YuG. DSM-probe fluorescence spectra analysis at serum albumin conformational changes investigation. The Jablonski Centennial Conference on Luminescence and Photophysics. Torun:Poland. 23-27 July 1998.
Zhydenko V, Gatash S, Dyubko T, Romodanova E. The conformation changes of human serum albumin under influence of freezing and laser irradiation. 7th Confer. On Methods and Applications of Fluorescence. Amsterdam, 16-19 sep 2001. P. 214.
Belyh IA, Zinchenko VD, Dyubko TS. Ozone influence on cholinesterase study by optical spectroscopy methods. Spectroscopy in special applications. Book of abstracts. 2003. 54p.
Published
2018-06-25
Cited
How to Cite
Belyh, I. A., Dyubko, T. S., & Zinchenko, V. D. (2018). The study of the ozone influence on bovine serum albumin and cholinesterase by the optical spectroscopy methods. Biophysical Bulletin, 2(13), 104-110. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/12476
Section
Action of physical agents on biological objects
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