Estimation of the thermal expansion coefficient of erythrocyte membrane surface by shift of curve of erythrocyte distribution by spherical index
Keywords:
erythrocytes, spherical index, plasma membranes, thermal expansion coefficient
Abstract
Authors proposed a new method for estimation of thermal expansion coefficient of erythrocyte membrane surface, basing on determination of erythrocyte distribution by spherical index at various temperatures. Using the small angle light scattering the erythrocyte osmotic fragility curves were obtained and then transited to curves of cell spherical index distribution by coordinate transformation and differentiation basing on elaborated physical and mathematical model of hypotonic hemolysis in non-permeating substance solution. Coefficient of erythrocyte membrane thermal expansion was determined by the shift of distribution maximum at temperature change. Obtained value of thermal expansion coefficient is in good concordance with known data from the literature, determined by micropipette method.
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References
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Livne A, Raz A.Erythrocyte fragility and potassium efflux as affected by temperature and hemolysing rate. FEBS Letter. 1971;16:99-101.
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Seeman P. The effect of membrane-strain rate and temperature on erythrocytes fragility and critical hemolytic volume. Biochem. Biophys. Acta. 1969;183:476-89.
Nishihara Y, Utsumi K. Diminished osmotic fragility and shape alterations of humanerythrocytes following the trearment with 1,1,1-trichloro-2,2-bis ethane (DDT)/Cell.Mol.iol. 1983;29:103-11.
Rand RP, Burton AC. Area and volume changes in hemolysis of single erythrocytes. J. Cell. Comp. Physiol. 1963;61:245-53.
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Gordienko EA, Gordienko OI. O mehanizme osmoticheskogo lizisa jeritrocitov. Kriobiologija. 1986;2:23-5. (in Russian)
Gordienko OI, Emec BG, Zhiljakoyva TA, Shejkin VI. Temperaturnaja zavisimost' diffuzionnoj pronicaemosti membran jeritrocitov v sredah s razlichnoj ionnoj siloj. Biol. membrany. 1985;2(3):310-4. (in Russian)
Livne A, Raz A.Erythrocyte fragility and potassium efflux as affected by temperature and hemolysing rate. FEBS Letter. 1971;16:99-101.
Richiery GV, Mel HC. Temperature effects on osmotic fragility and erythrocyte membrane. Biochem. Biophys. Acta.1985;813:41-50.
Murphy JR. Erythrocyte osmotic fragility and cell water-influence of pH and temperature. J.Lab.Clin. Med. 1969;74:319-24.
Waugh R, Evans EA. Temperature dependence of the elastic moduli of red blood cell membrane. Biophys. J. 1979;26:115-32.
Ivens I, Skeilak R. Mekhanika i termodinamika biologicheskikh membran. M.:Mir;1982. 304p. (in Russian)
Seeman P. The effect of membrane-strain rate and temperature on erythrocytes fragility and critical hemolytic volume. Biochem. Biophys. Acta. 1969;183:476-89.
Nishihara Y, Utsumi K. Diminished osmotic fragility and shape alterations of humanerythrocytes following the trearment with 1,1,1-trichloro-2,2-bis ethane (DDT)/Cell.Mol.iol. 1983;29:103-11.
Rand RP, Burton AC. Area and volume changes in hemolysis of single erythrocytes. J. Cell. Comp. Physiol. 1963;61:245-53.
Gordijenko OI, Kovalenko IF, Panina JuJe. Fizyko-matematychna model' ta eksperymental'ne vyznachennja javyshha gipotonichnogo gemolizu. Dopovidi NANU. 1998;'11:173-6. (in Ukrainian)
Gordijenko JeO, Gordijenko OI, Kovalenko IF, Panina JuJe, Aleksjejev OO. Fizyko-matematychnyj analiz ta eksperymental'ne vyznachennja shhil'nosti rozpodilu erytrocytiv donors'koi' i pupovynnoi' krovi ljudyny za indeksom sferychnosti. Biofizychnyj visnyk. 2000;1(6):75-8. (in Ukrainian)
Gordienko EA, Gordienko OI. O mehanizme osmoticheskogo lizisa jeritrocitov. Kriobiologija. 1986;2:23-5. (in Russian)
Gordienko OI, Emec BG, Zhiljakoyva TA, Shejkin VI. Temperaturnaja zavisimost' diffuzionnoj pronicaemosti membran jeritrocitov v sredah s razlichnoj ionnoj siloj. Biol. membrany. 1985;2(3):310-4. (in Russian)
Published
2018-06-25
Cited
How to Cite
Gordiyenko, O. I. (2018). Estimation of the thermal expansion coefficient of erythrocyte membrane surface by shift of curve of erythrocyte distribution by spherical index. Biophysical Bulletin, 2(13), 78-81. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/12418
Section
Cell biophysics
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