IR-spectroscopy investigation of Cu2+ ion action on conformational state of DNA
Abstract
This work studies the Cu2+ ion interaction ([Cu2+]/[P]=0,2-0,8) with the DNA macromolecule in aqueous solutions at different temperatures (T=26-930С) by the IR spectroscopy method. It is shown that Cu2+ ion binding to phosphate groups of the DNA molecule induce the increase of the intensity of the absorption spectrum in the region of vibrations of the sugar-phosphate chain that evidences the DNA transition into the compact state. The DNA transition into the compact state occurs in a rather narrow interval of Cu2+ ion concentrations ([Cu2+]/[P]=0,3-0,6). At the ratio [Cu2+]/[P]>0,6 Cu2+ ions disorder the DNA secondary structure at room temperature (T=260C). Effect of Cu2+ ions on the resistance of the DNA secondary structure for temperature changes were studied too. A nonlinear dependence of the DNA melting temperature on Cu2+ ion concentrations was obtained: the DNA melting temperature decreases significantly at [Cu2+]/[P]>0,2. This effect is due to the preferential binding of Cu2+ ions to nitrogen bases of DNA that destabilizes its secondary structure.
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References
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