Endangered species Crambe steveniana in vitro plant culture obtaining and the study of aseptic cultivation influence on its biochemistry
Keywords:
C. steveniana, biodiversity conservation, fatty acids, in vitro culture
Abstract
The study of endangered species Crambe steveniana aseptic in vitro culture establishing and definition of its fast microclonal propagation terms have been conducted. Fatty acid composition of vegetative parts of plants that were cultivated in vitro and in vivo along with their seeds was determined and compared. Seed surface sterilization was performed by the authors method with further morphogenic reaction of explants (lateral bud) on the presence in the medium of 6-benzylaminopurine (0,5–2 mg/L) study. Fatty acids content was determined using gas chromatography/mass spectrophotometry of fatty acid ethers obtained from seeds and green mass of plants that were cultivated in vitro and in vivo. Aseptic spouts culture of the studied species was established and optimal BA concentration (0,6 mg/L) in the medium was defined for fast multiplication of aseptic spouts. Fatty acid content increase was detected in the samples from aseptic plants compared with not aseptic ones. Also, high α-linolenic acid amount in the samples from both in vitro and in vivo grown plants was established. Significantly high erucic acid content was shown for seed samples.
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References
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Лось Д.А. Молекулярные механизмы холодоустойчивости растений // Вестник РАН. – 2005. – Т.75. – С. 338–345. /Los' D.A. Molekulyarnye mekhanizmy holodoustoychivosti rasteniy // Vestnik RAN. – 2005. – T.75. – S. 338–345./
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Сахно Л.О., Остапчук А.М., Клочко В.В., Кучук М.В. Жирнокислотний склад насіння ріпаку з трансгеном cyp11A1 цитохрому Р450SCC // Біотехнологія. – 2012. – Т.5, №5. – С. 27–33. /Sakhno L.O., Ostapchuk A.M., Klochko V.V., Kuchuk M.V. Zhyrnokyslotnyy sklad nasіnnya rіpaku z transgenom cyp11A1 tsitokhromu R450SCC // Bіotekhnologіya. – 2012. – T.5, №5. – S. 27–33./
Червона книга України. Рослинний світ / за ред. Я.П.Дідуха. – К.: Глобалконсалинг, 2009. – 900с. /Chervona knyga Ukrayiny. Roslynnyy svіt / za red. Ya.P.Dіdukha. – K.: Globalkonsaling, 2009. – 900s./
Artus N.N. Arsenic and cadmium phytoextraction potential of crambe compared with Indian mustard // Journal of Plant Nutrition. – 2006. – Vol. 29. – P. 667–679.
Bayram E., Yilmaz S., Hamat-Mecbur H. Nikita retrotransposon movements in callus cultures of barley (Hordeum vulgare L.) // Plant OMICS: Journal of Plant Molecular Biology & Omics. – 2012. – Vol.5 (3). – P. 211–217.
Belokurova V.B. Methods of biotechnology in system of efforts aimed at plant biodiversity preservation // Cytol. Genet. – 2010. – Vol.44, no 3. – P. 174–185.
Garces R., Mancha M. One-step lipid extraction and fatty acid methyl esters preparation from fresh plant tissues // Anal. Biochem. – 1993. – Vol.211. – P. 139–143.
Gaspar T., Franck T., Bisbis B. et al. Concepts in plant stress physiology. Application to plant tissue cultures // Plant Growth Regul. – 2002. – Vol.37. – P. 263–285.
Imran Mohammad, Farah Naz Talpur, Siradjuddin, Rasool Khan Leaf lipids of some edible plants from north-west Pakistan // J. Chem. Sok. Pak. – 2009. – Vol.31, no 3. – P. 492–497.
Lazzeri L., De Mattei F., Bucelli F., Palmieri S. Crambe oil – a potential new hydraulic oil and quenchant // Industrial Lubrication and Tribology. – 1997. – Vol.49, no 2. – P. 71–77.
Lyons J.M., Wheaton T.A., Pratt H.K. Relationship between the physical nature of mitochondrial membranes and chilling sensitivity in plant // Plant Physiol. – 1964. – Vol.39. – P. 262–268.
Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures // Physiol. Plant. – 1962. - Vоl.15. – P. 473–497.
Perry T.W., Kwolek W.F., Tookey H.L. et al. Crambe meal as a source of supplemental protein for growing beef cattle // Journal of animal science. – 1979. – Vol.48, no 4. – P. 758–763.
Prakhova T.Ya. New unconventional oilseed – Abyssinian Crambe // Bulletin of Altai state agricultural university. – 2013. – Vol.8, no 106. – P. 8–10.
Sandhir R., Khan M., Chahal A., Singh I. Localization of nervonic acid beta-oxidation in human and rodent peroxisomes: impaired oxidation in Zellweger syndrome and X-linked adrenoleukodystrophy // Journal of Lipid Research. – 1998. – Vol.39, no 11. – P. 2161–2171.
Sargent J.R., Coupland K., Wilson R. Nervonic acid and demyelinating disease // Medical Hypotheses. – 1994. – Vol.42, no 4. – P. 237–242.
Shah J. Lipids, lipases, and lipid modifying enzymes in plant disease resistance // Annual Review of Phytopathology. – 2005. – Vol.43. – P. 229–260.
Taylor D.C., Guo Y., Katavic V. et al. New seed oils for improved human and animal health and as industrial feedstocks: genetic manipulation of the Brassicaceae to produce oils enriched in nervonic acid // Modification of seed composition to promote health and nutrition / Ed. A.B.Krishnan. – American Society of Agronomy, 2009. – P. 219–233.
Todorovska E. Retrotransposons and their role in plant – genome evolution // Biotechnology & Biotechnological Equipment. – 2007. – Vol.21. – Р. 294–305.
Wang X., Li W., Li M., Welti R. Profiling lipid changes in plant response to low temperatures // Physiologia Plantarum. – 2006. – Vol.126. – P. 90–96.
Xiao-qin S., Hui P., Jian-lin G. et al. Fatty acid analysis of the seed oil in a germplasm collection of 94 species in 58 genera of Brassicaceae // Chem. Ind. of Forest Prod. – 2011. – Vol.31 (6). – P. 46–54.
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How to Cite
Пушкарьова, Н. О., Каліста, М. С., Белокурова, В. Б., & Кучук, М. В. (1). Endangered species Crambe steveniana in vitro plant culture obtaining and the study of aseptic cultivation influence on its biochemistry. The Journal of V.N.Karazin Kharkiv National University. Series «Biology», 27, 155-162. Retrieved from https://periodicals.karazin.ua/biology/article/view/8224
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PLANT PHYSIOLOGY
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