Cytogenetic analysis of antimutagenic action of sodium lignogumate at mutations induction by mitomycin C in Allium-test

В. М. Шкарупа

Keywords: antimutagenesis, sodium lignogumate, mitomycin C, Allium-test

Abstract

Cytogenetic effects of sodium lignogumate on genotoxity induced by mitomycin C in Allium-test were studied. Sodium lignogumate was tested at a concentration of 100 mg/l at the joint 72-hour exposure with mitomycin C. Analysis of the root meristem cells of Allium cepa L. seedlings was carried by ana-telophase method. It was found that sodium lignogumate had antimutagenic properties, reducing the frequency of mitomycin C induced chromosome aberrations by 64,51%. Complete elimination of multiaberrant cells was revealed. There was observed differential activity of the drug to reduce the frequency of chromosome aberrations of different types. The frequency of all types of "bridges" at the influence of sodium lignogumate reduced by 1.98 times, and the frequency of all types of fragments – by 8.03 times. There was revealed the complete elimination of double fragments (of chromosomal type), the frequency of single fragments (of chromatid type) decreased by 8.2 times. The effective elimination of multiaberrant cells and fragments indicates the presence of not only desmutagenіc but also other mechanisms of antimutagenic properties of sodium lignogumate. Therefore, multiple mechanisms were revealed for implementing antimutagenic properties of sodium lignogumate by mitomycin C induced mutagenesis in Allium-test.

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References

Бузлама А.В. Модулирующее влияние лигногумата на интенсивность процессов перекисного окисления и активность компонентов антиоксидантной защиты организма в экспериментальных условиях // Фундаментальные исследования. – 2010. – №9. – С. 36–40. /Buzlama A.V. Moduliruyushcheye vliyaniye lignogumata na intensivnost’ protsessov perekisnogo okisleniya i aktivnost komponentov antioksidantnoy zashchity organizma v eksperimentalnykh usloviyakh // Fundamentalnyye issledovaniya. – 2010. – №9. – S. 36–40./

Дворник А.С., Перерва Т.П., Кунах В.А. Антимутагенез як система захисту організму від ушкоджуючих факторів ендогенного та екзогенного походження // Цитологія і генетика. – 2004. – Т.38, №5. – С. 62–71. /Dvornyk A.S., Pererva T.P., Kunakh V.A. Antimutagenez yak systema zakhystu organizmu vid ushkodzhuyuchykh faktoriv endogennogo ta ekzogennogo pokhodzhennya // Tsitologiya i genetyka. – 2004. – T.38, №5. – S. 62–71./

Дурнев А.Д. Модификация мутационного процесса в клетках человека // Вестник РАМН. – 2001. – №10. – С. 70–76. /Durnev A.D. Modifikatsiya mutatsionnogo protsessa v kletkah cheloveka // Vestnik RAMN. – 2001. – №10. – S. 70–76./

Agar G., Taspinar M.S., Turan M. et al. Protective role of humic acids against dicamba-induced genotoxicity and DNA methylation in Phaseolus vulgaris L. // Acta Agriculturae Scandinavica. – Section B – Soil & Plant Science. – 2014. – Vol.64, no 2. – Р. 141–148.

Cozzi R., Nicolai M., Petricone P. et al. Desmutagenic activity of natural humic acids: Inhibition of mitomycin C and maleic hydrazide mutagenicity // Mutat. Res. – 1993. – Vol.299. – P.37–44.

Ferguson L.R., De Flora S. Multiple drug resistance, antimutagenesis and anticarcinogenesis // Mutat. Res. – 2005. – Vol.591, no 1–2. – P. 24–33.

Ferrara G., Loffredo E., Senesi N., Marcos R. Humic acids reduce the genotoxicity of mitomycin C in the human lymphoblastoid cell line TK6 // Mutat. Res. – 2006. – Vol.603, no 1. – P. 27–32.

Hseu Y-Ch., Chen S.-Ch., Chen Y.-L. et al. Humic acid induced genotoxicity in human peripheral blood lymphocytes using comet and sister chromatid exchange assay // Journal of Hazardous Materials. – 2008. – Vol.153. – P. 784–791.

Kubešová J., Turková V., Mikulcová A. et al. Antimutagenic and/or genotoxic effects of processed humic acids as tested upon S. cerevisiae D7 // Environ. Chem. Lett. – 2011. – Vol.9, iss.2. – P. 229–233.

Pena-Mendez E.M., Havel J., Patočka J. Humic substances – compounds of still unknown structure: applications in agriculture, industry, environment, and biomedicine // J. Appl. Biomed. – 2005. – No 3. – P. 13–24.

Poloskin R.B., Gladkov O.A., Osipova O.A., Yakimenko O.S. Comparable evaluation of biological activity of new liquid and dry modifications of the humic product “Lignohumate” // Functions of Natural Organic Matter in Changing Environment / Eds. J.Xu, Y.He, J.Wu. – Vol.I. – Dordrecht: Springer Netherlands, 2013. – P. 1095–1110.

Shkarupa V.M., Klymenko S.V., Talko V.V. Cytogenetic analysis of radioprotective properties of sodium lignogumate after γ-exposure in Allium-test // Problems of Radiation Medicine and Radiobiology. – 2014. – Vol.19. – P. 490–508.

Słoczyńska K., Powroźnik B., Pękala E., Waszkielewic A.M. Antimutagenic compounds and their possible mechanisms of action // J. Appl. Genetics. – 2014. – Vol.55. – P. 273–285.

Zhou X.P, ZhangY.Ch., Zhang Sh.W. et al. New progress in medical research of Bio-humic acid // Applied Mechanics and Materials. – 2012. – Vol. 138–139. – P. 1228–1233.