The features of cytotoxicity of cadmium ions ultra-low doses prolonged administration on rats skin, lungs, kidneys and cornea fibroblasts
Keywords:
skin, lungs, kidneys and cornea fibroblasts, cytotoxicity, ability of cells for adhesion and migration, apoptosis, ratio ADP/ATP, collagen, glycosaminoglycans, TGFβ1
Abstract
There have been studied the features of the cytotoxic effect of cadmium ions on the skin, lungs, kidneys and cornea fibroblasts, which were isolated into the culture from the organs of animals which received the sub low doses of Cd2+ with drinking water during 36 days, daily. Cytotoxicity was assessed by the ability of cells for adhesion and migration, their distribution according to early and late stages of apoptosis, as well as metabolic activity – by the ratio ADP/ATP; by the content of collagen, glycosaminoglycans and TGFβ1. There has been shown the greatest sustainability of the cornea and skin fibroblasts to the action of Cd2+ ions in the concentrations and terms of administration which were investigated. Cd2+ ions in relation to lung fibroblasts demonstrated the strongest cytotoxicity, already on the 15th day the administration of a dose Cd2+ 0.1 µg/kg/day. Administering of a dose Cd2+ 1 µg/kg/day resulted in increased cytotoxic effect on fibroblasts of the lung, but also a significant decrease in metabolic activity of cells. The cytotoxic effect of a dose Cd2+ 1 µg/kg/day on kidney fibroblasts was qualitatively similar to the effect of this dose on lung fibroblasts. Thus, kidney fibroblasts revealed the resistance to prolonged action of Cd2+ at a dose of 0.1 µg/kg/day.
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References
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Berlin M., Fredriesson B., Linge G. Bone marrow changes in chronic cadmium poisoning in rabbits // Journal of Occupational Medicine. – 1962. – Vol.4, issue 1. – P.48.
Bueno E. Isolation of corneal stromal fibroblasts with collagenase / Eds. A.Atala, R.Lanza. – Academic press, 2000. – P. 927–941.
Cadena-Herreraa D., Esparza-De Lara J.E. Validation of three viable-cell counting methods: manual, semi-automated, and automated // Biotechnology Reports. – 2015. – Vol.7. – P. 9–16.
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Elmore S. Apoptosis: a review of programmed cell death // Toxicol Pathol. – 2007. – Vol.35 (4). – P. 495–516.
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Gheisari Y., Soleimani M. Isolation of stem cells from adult rat kidneys // Biocell. – 2009. – Vol.33 (1). – P. 33–38.
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(https://www.thermofisher.com/order/catalog/product/A1142801)
Johri N., Jacquillet G., Unwin R.J. Heavy metal poisoning: The effects of cadmium on the kidney // Biology of Metals. – 2010. – Vol.23 (5). – P. 783–792.
Malmström А., Bartolini B., Thelin M.A. et al. Iduronic acid in chondroitin/dermatan sulfate: biosynthesis and biological function // J. Histochem. Cytochem. – 2012. – Vol.60 (12). – P. 916–925.
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Mason K.E., Brown J.A., Young J.O., Nesbit R.R. Cadmium-induced injury of the rat testis // The Anatomical Record. – 2005. – Vol.149, issue 1. – P. 135–147.
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Postlethwaite A.E., Shigemitsu H., Kanangat S. Cellular origins of fibroblasts: possible implications for organ fibrosis in systemic sclerosis // Curr Opin Rheumatol. – 2004. – Vol.16 (6). – P. 733–738.
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Rittié L., Fisher G.J. Isolation and culture of skin fibroblasts // Methods in Molecular Medicine. – 2005. – Vol.117. – P. 83–98.
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Siddiqui M.F. Cadmium induced renal toxicity in male rats, Rattus rattus // Eastern Journal of Medicine. – 2010. – Vol.15 (3). – P. 93–96.
Sriram G., Bigliardi P.L., Bigliardi-Qi M. Fibroblast heterogeneity and its implications for engineering organotypic skin models in vitro // European Journal of Cell Biology. – 2015. – Vol.94, issue 11. – P. 483–512.
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(http://www.abcam.com/ps/products/119/ab119558/documents/ab119558%20TGF%20beta%201%20Rat%20ELISA%20Kit%20v3%20(website).pdf)
Toxicological profile for cadmium. – U.S. Department of Health and Human Services, 2012. (http://www.atsdr.cdc.gov/toxprofiles/tp5.pdf)
Verordnung (EU) Nr. 488/2014 der Kommission vom 12. Mai 2014 zur Änderung der Verordnung (EG) Nr. 1881/2006 bezüglich der Höchstgehalte für Cadmium in Lebensmitteln. Bundesministerium für Umwelt, Naturschutz, Bau und Reaktorsicherheit.
(http://eur-lex.europa.eu/legal-content/DE/TXT/PDF/?uri=CELEX:32014R0488&from=DE)
von Zglinicki T., Edwall C. Very low cadmium concentrations stimulate DNA synthesis and cell growth // Journal of Cell Science. – 1992. – Vol.103. – P. 1073–1081.
Wang Bo, Du Yanli Cadmium and its neurotoxic effects // Oxidative Medicine and Cellular Longevity. – 2013. – Vol.4 (5). (DOI: 10.1155/2013/898034)
Wang Chunxin, Youle R.J. The role of mitochondria in apoptosis // Annual Review of Genetics. – 2009. – Vol.43. – P. 95–118.
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Сі, У., Пиріна, І. С., Кот, Ю. Г., Кот, К. В., & Перський, Є. Е. (1). The features of cytotoxicity of cadmium ions ultra-low doses prolonged administration on rats skin, lungs, kidneys and cornea fibroblasts. The Journal of V.N.Karazin Kharkiv National University. Series «Biology», 25, 332-340. Retrieved from https://periodicals.karazin.ua/biology/article/view/5987
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