Evaluation of genotype-environment interactions for non-polar lipids and fatty acids in chickpea (Cicer arietinum L.) seeds

doi:10.26565/2075-5457-2022-39-3

L. Relina The Plant Production Institute named after V.Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine https://orcid.org/0000-0003-2833-5841
O. Suprun The Plant Production Institute named after V.Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine https://orcid.org/0000-0002-7708-093X
R. Bohuslavskyi The Plant Production Institute named after V.Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine https://orcid.org/0000-0003-3145-4788
L. Vecherska The Plant Production Institute named after V.Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine https://orcid.org/0000-0003-3513-6701
O. Bezuhla The Plant Production Institute named after V.Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine https://orcid.org/0000-0002-1458-1630
L. Kobyzeva The Plant Production Institute named after V.Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine https://orcid.org/0000-0003-3067-7971
O. Vazhenina The Plant Production Institute named after V.Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine https://orcid.org/0000-0001-9471-5987
V. Kolomatska The Plant Production Institute named after V.Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine https://orcid.org/0000-0001-5408-4244
S. Ponurenko The Plant Production Institute named after V.Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine https://orcid.org/0000-0003-0397-636X
N. Ilchenko The Plant Production Institute named after V.Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine https://orcid.org/0000-0003-1080-1373

DOI: https://doi.org/10.26565/2075-5457-2022-39-3

Keywords: chickpea, oil, correlation, outlier, vegetation phases, analysis of variance, stability, ecovalence

Abstract

Genotype-environment (G × E) interactions for non-polar lipids and fatty acids were studied in 28 chickpea accessions. The total nonpolar lipid content was determined by Soxhlet procedure; fatty acid profiles were investigated by gas chromatography. There were strong negative correlations between oleic and linoleic acids and between oleic and linolenic acids. The correlation between linoleic and linolenic acids was positive and either strong or moderate. Correlations between the other acids were differently directed and of various strengths. Line Luh 99/11 turned out to be an outlier in relation to the other genotypes due to an unusually high content of stearic acid. Cultivar CDC Jade was an outlier because of too low content of stearic acid and too high content of linoleic acid. Accession UD0502195 was an outlier due to a higher content of palmitic acid. Accessions UD0500022 and UD0502195 were outliers due to the low content of total nonpolar lipids. The variability in the total nonpolar lipid content was not affected by the environment, but the environment contributions to the variability of oleic and linoleic acids were very high. There were only statistically significant differences in the oleic and linoleic acid amounts between the cultivation years. There was a positive correlation between the oleic acid content and the average air temperature during the “anthesis – maturity” period and a negative correlation between the linoleic acid content and the average temperature during this period. There was also a negative correlation between the oleic acid content and precipitation during the “anthesis – maturity” period and a positive correlation between the linoleic acid content and precipitation during this period. The palmitic acid content was the most responsive to environmental changes in cultivar CDC Vanguard and the most resistant in cultivar Krasnokutskiy 123. The stearic acid content was the most sensitive to environmental changes in cultivar ILC 3279 and the most irresponsive in accession UKR001:0502116. As to oleic and linoleic acids, line L 273-18 had the bi (plasticity) and S²di (stability) values coupled with the corresponding mean contents, meaning that this genotype may be adapted to decreased temperature. The ecovalence values (Wi²) for the total nonpolar lipids, palmitic, stearic and linolenic acids indicated that these characteristics were little responsive to environmental fluctuations. As to oleic and linoleic acids, Wi² values were much higher in many accessions, confirming the variability of these parameters depending on growing conditions. Having the highest Wi² values, accession Garbanzo 2 is expected to show high degrees of the G × E interactions for oleic and linoleic acids. S²di was positively correlated with Wi².

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Author Biographies

L. Relina, The Plant Production Institute named after V.Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine

Heroiv Kharkova Ave., 142, Kharkiv, Ukraine, 61060, lianaisaakovna@gmail.com

O. Suprun, The Plant Production Institute named after V.Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine

Heroiv Kharkova Ave., 142, Kharkiv, Ukraine, 61060, oleg.suprun@ukr.net

R. Bohuslavskyi, The Plant Production Institute named after V.Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine

Heroiv Kharkova Ave., 142, Kharkiv, Ukraine, 61060, boguslavr@meta.ua

L. Vecherska, The Plant Production Institute named after V.Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine

Heroiv Kharkova Ave., 142, Kharkiv, Ukraine, 61060, lyudmila_vecherska@ukr.net

O. Bezuhla, The Plant Production Institute named after V.Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine

Heroiv Kharkova Ave., 142, Kharkiv, Ukraine, 61060, olgabezuglaya61@gmail.com

L. Kobyzeva, The Plant Production Institute named after V.Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine

Heroiv Kharkova Ave., 142, Kharkiv, Ukraine, 61060, l.n.kobyzeva@gmail.com

O. Vazhenina, The Plant Production Institute named after V.Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine

Heroiv Kharkova Ave., 142, Kharkiv, Ukraine, 61060, vajeninaolga29@gmail.com

V. Kolomatska, The Plant Production Institute named after V.Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine

Heroiv Kharkova Ave., 142, Kharkiv, Ukraine, 61060, valeriya.kolom@gmail.com

S. Ponurenko, The Plant Production Institute named after V.Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine

Heroiv Kharkova Ave., 142, Kharkiv, Ukraine, 61060, serhii_ponurenko@ukr.net

N. Ilchenko, The Plant Production Institute named after V.Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine

Heroiv Kharkova Ave., 142, Kharkiv, Ukraine, 61060, masovianalizy@gmail.com

References

Barker G.C., Larson T.R., Graham I.A. et al. (2007). Novel insights into seed fatty acid synthesis and modification pathways from genetic diversity and quantitative trait loci analysis of the Brassica C genome. Plant Physiology, 144(4), 1827–1842. https://doi.org/10.1104/pp.107.096172

Benelli V., Allen F., Wang M. (2017). Variability in seed oil content and fatty acid composition, phenotypic traits and self-incompatibility among selected niger germplasm accessions. Plant Genetic Resources, 15(4), 348–354. https://doi.org/10.1017/S1479262115000702

Bernard M.H. (2014) Molecular cloning and functional characterization of genes involved in the biosynthesis of polyunsaturated fatty acids in oat (Avena sativa L.). Thesis for the Degree of Master of Science, University of Saskatchewan. HARVEST Repository: http://hdl.handle.net/10388/ETD-2014-04-1543

Brock J.R., Scott T., Lee A.Y. et al. (2020). Interactions between genetics and environment shape Camelina seed oil composition. BMC Plant Biology, 20, 423. https://doi.org/10.1186/s12870-020-02641-8

Byfield G.E., Upchurch R.G. (2007). Effect of temperature on delta-9 stearoyl-ACP and microsomal omega-6 desaturase gene expression and fatty acid content in developing soybean seeds. Crop Science, 47(4), 1698–1704. https://doi.org/10.2135/cropsci2006.04.0213

Cahoon E.B., Shanklin J. (2000). Substrate-dependent mutant complementation to select fatty acid desaturase variants for metabolic engineering of plant seed oils. Proc. Natl. Acad. Sci. USA., 97(22), 12350–12355. https://doi.org/10.1073/pnas.210276297

Chepur S.S. (2015). Biometry. Uzhhorod: Publishing House UzhNU “Hoverla". 40 p. (in Ukrainian)

Dabbou S., Rjiba I., Echbili A. et al. (2010). Effect of controlled crossing on the triglyceride and fatty acid composition of virgin olive oils. Chemistry & Biodiversity, 7(7), 1801–1813. https://doi.org/10.1002/cbdv. 200900385

Dakhma W.S., Zarrouk M., Cherif A. (1995). Effects of drought stress on lipids in rape leaves. Phytochemistry, 40(5), 1383–1386. https://doi.org/10.1016/0031-9422(95)00459-K

Dar A.A., Choudhury A.R., Kancharla P.K. et al. (2017). The FAD2 gene in plants: occurrence, regulation, and role. Frontiers in Plant Science, 8, 1789. https://doi.org/10.3389/fpls.2017.01789

De la Rosa R., Arias-Calderón R., Velasco L., León L. (2016). Early selection for oil quality components in olive breeding progenies. European Journal of Lipid Science and Technology, 118(8), 1160–1167. https://doi.org/10.1002/ejlt.201500425

Eberhart S.A., Russell W.A. (1966). Stability parameters for comparing varieties. Crop Science, 6(1), 36–40. https://doi.org/10.2135/cropsci1966.0011183X000600010011x

Falcone D.L., Ogas J.P., Somerville C.R. (2004). Regulation of membrane fatty acid composition by temperature in mutants of Arabidopsis with alterations in membrane lipid composition. BMC Plant Biology, 4, 17. https://doi.org/10.1186/1471-2229-4-17

Falconer D.S., Mackay T.F.C. (1996). Introduction to Quantitative Genetics. 4th edition. Harlow, Essex, UK: Addison Wesley Longman, 448 p.

Harwood J.L. (1996). Recent advances in the biosynthesis of plant fatty acids. Biochimica et Biophysica Acta – Lipids and Lipid Metabolism, 1301(1–2), 7–56. https://doi.org/10.1016/0005-2760(95)00242-1

Harwood J.L. (2005). Fatty acid biosynthesis. In: Plant Lipids: Biology, Utilisation and Manipulation. Ed. D.J. Murphy. Oxford: Blackwell Publishing. P. 27–66.

Hernández M.L., Padilla M.N., Mancha M., Martínez-Rivas J.M. (2009). Expression analysis identifies FAD2-2 as the olive oleate desaturase gene mainly responsible for the linoleic acid content in virgin olive oil. J. Agric. Food Chem., 57(14), 6199–6206. https://doi.org/10.1021/jf900678z

Hernández M.L., Velázquez-Palmero D., Sicardo M.D. et al. (2018). Effect of a regulated deficit irrigation strategy in a hedgerow ‘Arbequina’ olive orchard on the mesocarp fatty acid composition and desaturase gene expression with respect to olive oil quality. Agricultural Water Management, 204, 100–106. https://doi.org/10.1016/j.agwat.2018.04.002

Islam M.S., Rahman L., Alam M.S. (2009). Correlation and path coefficient analysis in fat and fatty acids of rapeseed and mustard. Bangladesh Journal of Agricultural Research, 34(2), 247–253. https://doi.org/10.3329/bjar.v34i2.5796

ISO 16269-4:2010. Statistical interpretation of data – Part 4: Detection and treatment of outliers.

Ivanenko E.G., Volf V.G., Litun P.P. (1978). To the method of studying the plasticity of varieties. Selektsiya i Semenovodstvo, 40, 16–25. (in Russian)

Izquierdo N.G., Aguirrezábal L.A.N., Andrade F.H. et al. (2006). Modeling the response of fatty acid composition to temperature in a traditional sunflower hybrid. Agronomy Journal, 98(3), 451–461. https://doi.org/10.2134/agronj2005.0083

Juhaimi F.A., Uslu N., Babiker E.E. et al. (2019). The effect of different solvent types and extraction methods on oil yields and fatty acid composition of safflower seed. Journal of Oleo Science, 68(11), 1099–1104. https://doi.org/10.5650/jos.ess19131

Kargiotidou A., Deli D., Galanopoulou D. et al. (2008). Low temperature and light regulate delta 12 fatty acid desaturases (FAD2) at a transcriptional level in cotton (Gossypium hirsutum). Journal of Experimental Botany, 59(8), 2043–2056. https://doi.org/10.1093/jxb/ern065

Lanna A.C., José I.C., Oliveira M.G. de A. et al. (2005). Effect of temperature on polyunsaturated fatty acid accumulation in soybean seeds. Brazilian Journal of Plant Physiology, 17(2), 213–222. https://doi.org/10.1590/S1677-04202005000200004

Lehrian D.W., Keeney P.G., Butler D.R. (1980). Triglyceride characteristics of cocoa butter from cacao fruit matured in a microclimate of elevated temperature. Journal of the American Oil Chemists’ Society, 57, 66–69. https://doi.org/10.1007/BF02674362

Liu X., Huang B. (2004). Changes in fatty acid composition and saturation in leaves and roots of creeping bentgrass exposed to high soil temperature. Journal of the American Society for Horticultural Science, 129(6), 795–801. https://doi.org/10.21273/JASHS.129.6.0795

Liu B., Sun Y., Xue J. et al. (2019). Stearoyl-ACP Δ9 desaturase 6 and 8 (GhA-SAD6 and GhD-SAD8) are responsible for biosynthesis of palmitoleic acid specifically in developing endosperm of upland cotton seeds. Frontiers in Plant Science, 10, 703. https://doi.org/10.3389/fpls.2019.00703

Lule D., Tesfaye K., Mengistu G. (2014). Genotype by environment interaction and grain yield stability analysis for advanced triticale (x. Triticosecale Wittmack) genotypes in western Oromia, Ethiopia. Ethiopian Journal of Science, 37(1), 63–68.

Maeda H., Sage T.L., Isaac G. et al. (2008). Tocopherols modulate extraplastidic polyunsaturated fatty acid metabolism in Arabidopsis at low temperature. The Plant Cell, 20(2), 452–470. https://doi.org/10.1105/tpc.107.054718

Martínez-Force E., Álvarez-Ortega R., Cantisán S., Garcés R. (1998). Fatty acid composition in developing high saturated sunflower (Helianthus annuus) seeds: maturation changes and temperature effect. J. Agric. Food Chem., 46(9), 3577–3582. https://doi.org/10.1021/jf980276e

Martínez-Rivas J.M., García-Díaz M.T., Mancha M. (2000). Temperature and oxygen regulation of microsomal oleate desaturase (FAD2) from sunflower. Biochem. Soc. Trans., 28(6), 890–892.

Matteucci M., D’Angeli S., Errico S. et al. (2011). Cold affects the transcription of fatty acid desaturases and oil quality in the fruit of Olea europaea L. genotypes with different cold hardiness. Journal of Experimental Botany, 62(10), 3403–3420. https://doi.org/10.1093/jxb/err013

Mekki B.B., El-Kholy M.A., Mohamad E.M. (1999). Yield, oil and fatty acids contents as affected by water deficit and potassium fertilization in two sunflower cultivars. Egyptian Journal of Agronomy, 21(1), 67–85.

Moghadam H.R.T., Zahedi H., Ghooshchi F. (2011). Oil quality of canola cultivars in response to water stress and super absorbent polymer application. Pesqui. Agropecu. Trop., 41(4), 579–586. https://doi.org/10.5216/pat.v41i4.13366

Mustiga G.M., Morrissey J., Stack J.C. et al. (2019). Identification of climate and genetic factors that control fat content and fatty acid composition of Theobroma cacao L. beans. Front Plant Sci., 10, 1159. https://doi.org/10.3389/fpls.2019.01159

Peisker K.V. (1964). A rapid semi-micro method for preparation of methyl esters from triglycerides using chloroform, methanol, sulphuric acid. Journal of the American Oil Chemists' Society, 41(1), 87–88. https://doi.org/10.1007/BF02661915

Prokhorova M.I. (1982). Methods of biochemical studies (lipid and energy metabolism. Leningrad: Publishing House of Leningrad University. 327 p. (in Russian)

Ripa V., De Rose F., Caravita M.A. et al. (2008). Qualitative evaluation of olive oils from new olive selections and effects of genotype and environment on oil quality. Advances in Horticultural Science, 22(2), 95–103.

Salimonti A., Carbone F., Romano E. et al. (2020). Association study of the 5′UTR intron of the FAD2-2 gene with oleic and linoleic acid content in Olea europaea L. Front. Plant Sci., 11, 66 https://doi.org/10.3389/fpls.2020.00066

Selyaninov G.T. (1928). About agricultural estimate of climate. Trudy po Selskokhosyaystvennoy Meteorologii, 20, 165–177. (in Russian)

Shen Q., Zhao J.X., Qiu X.B. et al. (2018). Research on influence of environment factors to yield and quality traits of Perilla frutescen. Zhongguo Zhong Yao Za Zhi, 43(20), 4033–4043. https://doi.org/10.19540/j.cnki.cjcmm.20180820.007. (in Chinese)

Stefansson B.R., Storgaard A.K. (1969). Correlations involving oil and fatty acids in rapeseed. Canadian Journal of Plant Science, 49(5), 573–580. https://doi.org/10.4141/cjps69-099

Tanhuanpaa P., Schulman A. (2002). Mapping of genes affecting linolenic acid content in Brassica rapa ssp. oleifera. Molecular Breeding, 10, 51–62. https://doi.org/10.1023/A:1020357211089

Timoszuk M., Bielawska K., Skrzydlewska E. (2018). Evening primrose (Oenothera biennis) biological activity dependent on chemical composition. Antioxidants, 7(8), 108. https://doi.org/10.3390/antiox7080108

United Nations Data Retrieval System. (2022). http://data.un.org/Data.aspx?d=FAO&f=itemCode%3A191

Vickery M.L., Vickery B. (1981). Introduction. In: Secondary Plant Metabolism. Palgrave, London. 1–19. https://doi.org/10.1007/978-1-349-86109-5_1

Wallace T.C., Murray R., Zelman K.M. (2016). The nutritional value and health benefits of chickpeas and hummus. Nutrients, 8(12), 766. https://doi.org/10.3390/nu8120766

Wang M.L., Khera P., Pandey M.K. et al. (2015). Genetic mapping of QTLs controlling fatty acids provided insights into the genetic control of fatty acid synthesis pathway in peanut (Arachis hypogaea L.). PLoS One, 10(4), e0119454. https://doi.org/10.1371/journal.pone.0119454

Wintgens J.N. (1992). Influence of genetic factors and agroclimatic conditions on the quality of cocoa: 2nd International Congress on Cocoa and Chocolate, May 1991. Munich: Nestec Limited, Agricultural Services. 55 p.

Wricke G. (1965). Zur berechning der okovalenz bei sommerweizen und hafer. Z. Pflanzenzuchtung, 52, 127–138. (in German)

Zhang Y., Maximova S.N., Guiltinan M.J. (2015). Characterization of a stearoyl-acyl carrier protein desaturase gene family from chocolate tree, Theobroma cacao L. Frontiers in Plant Science, 6, 239. https://doi.org/10.3389/fpls.2015.00239

Zhou X.P., Wang M.Y., Feng L. et al. (2017). Cloning and expression of HaFAD2 2 gene from Helianthus annuus L. Acta Botanica Boreali-Occidentalia Sinica, 37(5), 845–850. (in Chinese)