Effect of various concentrations of the cryoprotectant DMSO on safety, viability and content of reactive oxygen species in cord blood nucleated cells during cryopreservation
Keywords:
cord blood, nucleated cells, cryopreservation, reactive oxygen speciesAbstract
The paper presents the results of the study of development of approaches to assess the number of cord blood nucleated cells (NC) with excessive reactive oxygen species (ROS) content with the fluorescent dye 2',7'-dihlordigidrofluorestsein diacetate by flow cytometry. Both exposure of NC for one hour with various concentrations of penetrating cryoprotectant DMSO and their cryopreservation resulted in reduced cell safety and viability that may be caused by inhibition of cell antioxidant system with the following accumulation of excessive content of ROS in cells. The results obtained showed the necessity of the addition of antioxidants into cryoprotective medium to prevent oxidative stress inside cells for improving cryopreservation techniques.
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