Variability of cultural and morphological traits of Dunaliella salina Teod. from different habitats

О. С. Пасюга
С. П. Антоненко
В. П. Комариста
А. Н. Рудась

Keywords: Dunaliella salina; -carotene; nitrogen; phosphorus; isolate; cultivation

Abstract

Deficiency of nitrogen and phosphorus induced b-carotene accumulation in the cells of 35 clonal cultures of D. salina isolated from 6 habitats in Ukraine and 1 in Russia. Nitrate and phosphate re-supplied into the medium decreased cellular b-carotene content and cell size. Repeated passage into the medium without nutrients recovered inducible b-carotene level; the dimensional characteristics of the cells recovered partly. Isolates from different habitats (except one) broke up into 2 groups: low carotene – small celled – fast growing and high carotene – large celled – slow growing. Peculiarities of clonal cultures depended on habitats, where they were isolated from, and persisted for 3 passages, i.e. inherited. Each 5 isolates from the same habitat did not differ, i.e. D. salina populations were homogenous within a single habitat, at least at the time and location of sampling. The ability to b-carotene accumulation induced by the deficit of nutrients can be extrapolated to D. salina species on the whole, at least to the populations originated from Ukraine and lake Baskunchak in Russia.

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References

Antonenko S.P., Dogadina T.V., Komaristaya V.P. Morphometric signs variability of Dunaliella salina in culture conditions // Ecology of Sea. – 2010. – Spec. issue 81: Management of hydrobionts biosynthesis. – P. 5–12. (in Russian)

Ben-Amotz A., Katz A., Avron M. Accumulation of β-carotene in halotolerant algae: purification and characterization of β-carotene-rich globules from Dunaliella bardawil // J. Phycol. – 1982. – Vol.18, N4. – P. 529–537.

Bozhkov A.I., Komaristaya V.P. Lipid-carotenoid metabolism in the cells of Dunaliella Teod. under different culture conditions // Algologia. – 2003. – Vol.13, N2. – P. 137–147. (in Russian)

Borowitzka M.A. Dunaliella: biology, production, and markets // Handbook of Microalgal Culture: Applied Phycology and Biotechnology. – 2013. – P. 359–368.

Borowitzka M.A., Siva Ch.J. The taxonomy of the genus Dunaliella (Chlorophyta, Dunaliellales) with emphasis on the marine and halophilic species // J. Appl. Phycol. – 2007. – Vol.19, N5. – P. 567–590.

Catalogue of microalgal cultures in the collections of the USSR. – Moscow: Inst. of Plant Physiol., 1991. – 226p. (in Russian)

Cifuentes A.S., Gonzalez M., Conejeros M. et al. Growth and carotenogenesis in eight strains of Dunaliella salina Teodoresco from Chile // Journal of Applied Phycology. – 1992. – Vol.4, N2. – P. 111–118.

Collection of microalgal cultures (liquid cultures) // R.G.Gevorgiz, Y.V.Bryantseva, A.B.Borovkov. – Sevastopol: A.O.Kovalevskiy Institute of Biology of Southern Seas, Department of Biotechnology and Phytoresources, 2007. – 53p. (in Russian)

Dogadina T.V., Komaristaya V.P. Catalog of microalgal cultures in the collection of Botany Department of V.N. Karazin Kharkiv National University // Journal of Kharkov National Agrarian University, Ser. Bіol. – 2005. – Vol.2, N7. – P. 121–130. (in Russian)

Fogg G.F., Thake B. Algal cultures and phytoplankton ecology. – Wisconsin: The University of Wisconsin Press, 1987. – 273p.

Garcia F., Freile-Pelegrin Y., Robledo D. Physiological characterization of Dunaliella sp. (Chlorophyta, Volvocales) from Yucatan, Mexico // Bioresource technology. – 2007. – Vol.98, N7. – P. 1359–1365.

Gomez P.I., Gonzalez M.A. Genetic polymorphism in eight Chilean strains of the carotenogenic microalga Dunaliella salina Teodoresco (Chlorophyta) // Biological Research. – 2001. – Vol.34, N1. – P. 23–30.

Gomez P.I., Gonzalez M.A. Genetic variation among seven strains of Dunaliella salina (Chlorophyta) with industrial potential, based on RAPD banding patterns and on nuclear ITS rDNA sequences // Aquaculture. – 2004. – Vol.233, N1. – P. 149–162.

Gomez P.I., Gonzalez M.A., Becerra J. Quantity and quality of -carotene produced by two strains of Dunaliella salina (Teodoresco 1905) from the North of Chile // Boletín de la Sociedad Chilena de Química. – 1999. – Vol.44, N4. – P. 463–468.

IARC Working Group on the Evaluation of Cancer Preventive Agents: IARC Handbook on Cancer Prevention. – Vol. 2: Carotenoids. Lyon, France: IARC, 1998. – 326p.

Jayappriyan K.R., Rajkumar R., Rengasamy R. Unusual occurrence of non carotenogenic strains of Dunaliella bardawil and Dunaliella parva in India // Journal of Basic Microbiology. – 2011. – Vol.51, N5. – P. 473–483.

Komaristaya V.P., Antonenko S.P., Rudas A.N. Cultivation of Dunaliella salina Teod. at suboptimal concentrations of nitrogen and phosphorus and their exclusion from the medium // Algologia. – 2010. – Vol.20, N1. – P. 42–55. (in Russian)

Lerche W. Untersuchungen über Entwicklung und Fortpflanzung in der Gattung Dunaliella // Arch. Protistenk. – 1936/1937. – Bd. 88. – S. 236-268.

Loeblich L.A. Photosynthesis and pigments influenced by light intensity and salinity in the halophile Dunaliella salina (Chlorophyta) // J. Mar. Biol. Ass. UK. – 1982. – Vol.62, N3. – P. 493–508.

Marín N., Morales F., Lodeiros C., Tamigneaux E. Effect of nitrate concentration on growth and pigment synthesis of Dunaliella salina cultivated under low illumination and preadapted to different salinities // Journal of Applied Phycology. – 1998. – Vol.10, N4. – P. 405–411.

Massyuk N.P. Morphology, systematics, ecology, geographical distribution of the genus Dunaliella Teod. and the prospects for its practical use. – Kiev: Nauk. dumka, 1973. – 244p. (in Russian)

Narváez-Zapata J.A., Rojas-Herrera R., López-Uc Y., Sánchez-Estudillo L. Different physiological responses influenced by salinity in genetically related Dunaliella salina isolates // Biotechnology Letters. – 2011. – Vol.33, N5. – P. 1021–1026.

Olmos J., Paniagua J., Contreras R. Molecular identification of Dunaliella sp. utilizing the 18S rDNA gene // Letters in Applied Microbiology. – 2000. – Vol.30, N1. – P. 80–84.

Olmos-Soto J., Paniagua J., Contreras R., Trujillo L. Molecular identification of β-carotene hyperproducing strains of Dunaliella from saline environments using species-specific oligonucleotides // Biotechnology Letters. – 2002. – Vol.24, N5. – P. 365–369.

Oren A. A hundred years of Dunaliella research: 1905–2005 // Saline systems. – 2005. – Vol.1, N2. – P. 1–14.

Polle J.E., Struwe L., Jin E. Identification and characterization of a new strain of the unicellular green alga Dunaliella salina (Teod.) from Korea //Journal of Microbiology and Biotechnology. – 2008. – Vol.18, N5. – P. 821–827.

Semenenko V.E., Abdullaev A.A. Parametric control of the biosynthesis of β-carotene in Dunaliella salina cells under the conditions of intensive culture // Plant Physiol. – 1980. – Vol.27, N1. – P. 31–41. (in Russian)

Tsoglin L.N., Vladimirova M.G., Semenenko V.E. Mathematical and experimental modeling of autoselection in microalgae at cultivation under flow // Plant Physiol. – 1970. – Vol.17, N6. – P. 1129–1139. (in Russian)