Efficiency of house poultry sperm cryopreservation by the Kharkiv technology
Abstract
The article presents the results of comparing the effectiveness of cryopreservation of poultry sperm according to the Kharkiv and German technology and the physiological characteristics of sperm of male birds of different species. Ejaculates from experimental birds were obtained 3 times a week by dorso-abdominal massage. In freshly ejaculates, motility of sperm cells and the number of pathological forms of germ cells in percent were determined in the Sperm Vision system (Minitube, Germany). After freezing and thawing, the sperm motility was determined in percent. The integrity of the plasma membranes of bird sperm before and after cryopreservation was determined on a DAKO Galaxy cytometer. Parts of the ejaculates that were diluted with the control diluent were frozen in 0.25 ml paillettes in an automatic freezer Biofreeze BV-65 (Consarctic Entwicklung Und Handels GmbH, Germany). Parts of ejaculates that were diluted with the diluent being developed were frozen in the form of coated pellets of 0.25 ml in the freezer developed for mammalian sperm of Kharkiv technology. The effectiveness of the Kharkiv technology of cryopreservation of sperm, which was modified by us for bird sperm, provided the best physiological characteristics of the ejaculates of poultry after thawing than the German technology. The mobility of rooster sperm after deconservation was 4.24% more than when applying the German technology with an increase in membrane preservation by 8.6%. The cryoresistance of quail sperm was also higher when applying the Kharkiv technology by 3.24% in mobility and by 3.26% in membrane-stabilizing properties. The efficiency of cryopreservation of the sperm of the czars according to the Kharkiv technology in lined granules was higher than when applying the German technology in mobility of sperm cells by 4.44%, in the integrity of plasma membranes of germ cells by 8.87%. The physiological characteristics of thawed turkey sperm, which was harvested according to the Kharkiv technology, surpassed the German equivalent by 7.04% in mobility and 2.65% in preservation of sperm membrane.
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