The influence of flavin mononucleotide on interaction in ethidium bromide-DNA system

A. A. Gerus Institute for Radiophysics and Electronics of NAS of Ukraine, 12 Acad. Proskury str., Kharkiv, 61085, Ukraine
A. V. Fomin Institute for Radiophysics and Electronics of NAS of Ukraine, 12 Acad. Proskury str., Kharkiv, 61085, Ukraine
N. A. Gladkovskaya Institute for Radiophysics and Electronics of NAS of Ukraine, 12 Acad. Proskury str., Kharkiv, 61085, Ukraine
E. G. Bereznyak Institute for Radiophysics and Electronics of NAS of Ukraine, 12 Acad. Proskury str., Kharkiv, 61085, Ukraine
E. V. Dukhopelnikov Institute for Radiophysics and Electronics of NAS of Ukraine, 12 Acad. Proskury str., Kharkiv, 61085, Ukraine
A. S. Khrebtova Institute for Radiophysics and Electronics of NAS of Ukraine, 12 Acad. Proskury str., Kharkiv, 61085, Ukraine
A. V. Zinchenko Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, 23 Pereyaslavskaya str., Kharkov, 61015, Ukraine

Keywords: competitive binding, flavin mononucleotide, ethidium bromide, DNA, calorimetry, spectrophotometry

Abstract

The study of intercalator ethidium bromide (EB) interaction with DNA in the presence of vitamin B2 analogue flavin mononucleotide (FMN) is carried out using the differential scanning calorimetry (DSC) and spectrophotometry (SF). Calorimetric investigations indicate that addition of FMN leads to the increase of DNA melting temperature, enthalpy, entropy and free energy in its complex with EB. The spectra of binary (EB-DNA, DNA-FMN) and ternary (FMN-EB-DNA) systems at different temperatures are obtained. Comparison of the experimental spectra shows that the intensity of the summary spectrum (EB-DNA+FMN-DNA) is higher than the intensity of the ternary system spectrum obtained experimentally. It should be noted that the intensity difference rises with the temperature increasing. The observed by DSC and SF effects can be explained by the increase of the concentration of ethidium
bromide associated with DNA in the presence of FMN.

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