Oposeredkovana metilenovim sin m fotodinam chna naktivats ya museum that kl n chnogo штамів staphylococcus aureus

Abstract

Photodynamic inactivation of Staphylococcus aureus collection and clinical strains was realized with methylene blue (6-40 µg /ml) and laser irradiation (λ=660 nm, light irradiance (energy density ?) 25 mW/сm², exposure time 10, 20, 30 min., doses 15,3, 30,6 and 46 J/сm², respectively). It was shown, that the bactericidal effect was not dependent on the concentration of methylene blue in range 10-40 µg/ml but it was dependent on the light dose. The optimal dose for bacterial reduction was 30.6 J/сm². The dynamic of cells damage was the similar for examined strains and achieved 3.5-4.9 lg. It was found the difference in the damage intensity of the strains at methylene blue range 2-20 µg/ml: the maximum effect on Staphylococcus aureus АТСС 6538 was with 6 µg/ml (5.6 lg reduction) and on Staphylococcus аureus 48011 - with 10 µg/ml (4,96 lg reduction). In the presents of blood serum the photodynamic effect was reasonably blockaded.