Photobactericidal properties of thermosensitive hydrogel nanocomposite with methylene blue
Abstract
A hydrogel nanocompozite was synthesized, which released deposited methylene blue at temperature of human body, preserving its photosensitizing activity. The hydrogel samples were contaminated by Staphylococcus aureus or Escherichia coli and after 24 h incubation at 37 °С irradiated with diode laser, l = 658 nm at the doses of 15 J/cm2 and 30 J/cm2, respestively. Swabs from the hydrogel samples surfaces were studied using CFU counts on plain agar. Evident photobactericidal activity of the nanocomposite with methylene blue was revealed, both towards gram-positive and gram-negative microorganisms. Availability of the proposed hydrogel composition exploiting as thermally controlled vehicle for methylene blue delivery to the inflammation nidus in antimicrobial photodynamic therapy was concluded.
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