Effect of low intensity microwave radiation and magnetic field on the state of chromatin in cell nuclei and contents of regulatory protein cyclin А in cells

  • Константин Андреевич Кузнецов
  • Дмитрий Борисович Мирошник
  • Владимир Николаевич Пасюга
  • Дмитрий Дмитриевич Иванченко
  • Ольга Юрьевна Ключевская
  • Ростислав Степанович Стойка
  • Юрий Георгиевич Шкорбатов
Keywords: Еlectromagnetic radiation, magnetic field, cyclin A, chromatin, heterochromatin

Abstract

The influence of low-level microwave radiation (frequency 36,64 GHz, power density 1 W/m2) and magnetic field (induction 25 mT) on changes of chromatin condensation rate in human buccal epithelium cells and contents of protein cyclin A in macrophage cells of mice J774.2. The chromatin state was evaluated by orcein staining, contents of proteins – by western-blotting. The increase of chromatin condensation and content of protein cyclin A in cells induced by the microwave irradiation and magnetic field were detected.

Downloads

Download data is not yet available.

References

Грабіна В.А. Пристрій впливу обертовим магнітним полем вихрового типу на біологічні об'єкти / В.А.Грабіна, А.М.Коробов, В.А.Луньова, Г.К.Лала.- Патент України №38540, 2009, Бюл. №1.

Banik S. Bioeffects of microwave – a brief review / S.Banik, S.Bandyopadhyay, S.Ganguly // Bioresource Technology.– 2003.– Vol.87.– P.155-159.

Bhat M.A. Effects of electromagnetic waves emitted by mobile phones on male fertility // Computer Engineering & Intelligent Systems.– 2013.– Vol.4, Issue 3.– P.51-64.

Elliott P.Mobile phone base stations and early childhood cancers: case-control study / P.Elliott, M.B.Toledano, J.Bennett et al. // Brit. Med. J.– 2010.– Vol.340.- P.c3077.

Harashima H. Cell cycle control across the eukaryotic kingdom / H.Harashima, N.Dissmeyer, A.Schnittger // Trends in Cell Biology.– 2013.– Vol.23, Issue 7.– P.345–356.

Hardell L. Epidemiological evidence for an association between use of wireless phones and tumor diseases / L.Hardell, M.Carlberg, K.Hansson-Mild // Pathophysiology.– 2009.– Vol.16.– P.113-122.

Hardell L. Use of mobile phones and cordless phones is associated with increased risk for glioma and acoustic neuroma / L.Hardell, M.Carlberg, K.Hansson-Mild // Pathophysiology.– 2013.– Vol.20.– P.85-110.

Hutter H.-P. Subjective symptoms, sleeping problems, and cognitive performance in subjects living near mobile phone base stations / H.-P.Hutter, H.Moshammer, P.Wallner, M.Kundi // Occup. Environ. Med.– 2006.– Vol.63.– P.307-313.

Kesari K.K. Cell phone radiation exposure on brain and associated biological systems / K.K.Kesari, M.H.Siddiqui, R.Meena et al. // Indian J. Exp. Biol.– 2013.– Vol.51.– P.187-200.

Kumar S. The therapeutic effect of a pulsed electromagnetic field on the reproductive patterns of male Wistar rats / S.Kumar, K.K.Kesari, J.Behari // Clinics.– 2011.– Vol.66.– P.1237-1245.

Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4 // Nature.– 1970.– Vol.227.– P.680-685.

Lai H. Interaction of microwaves and a temporally incoherent magnetic field on single and double DNA strand breaks in rat brain cells / H.Lai, N.P.Singh // Electromagnetic Biology and Medicine.– 2005.– Vol.24.– P.23-29.

Levallois P. Hypersensitivity of human subjects to environmental electric and magnetic field exposure: a review of the literature // Environmental Health Perspectives.– 2002.– Vol.110, Suppl.4.– P.613-618.

Markov M.S. Electromagnetic fields and life // J. Electr. Electron. Syst.– 2014.– Vol.3.- P. 119.

Martynyuk V.S. Interference of the mechanisms of influence that weak extremely low-frequency electromagnetic fields have on the human body and animals / V.S.Martynyuk, Yu.V.Tseysler, N.A.Temuryants // Izvestia, Atmospheric and Ocean Physics (Перевод журнала Известия РАН. Физика атмосферы и океана). – 2012.– Vol.48, №8.– P.832-846.

Poulsen A.H. Mobile phone use and the risk of skin cancer: a nationwide cohort study in Denmark / A.H.Poulsen, S.Friis, C.Johansen et al. // Amer. J. Epidemiol.– 2013.– Vol.178.- P.190-197.

Shckorbatov Y.G. He-Ne laser light induced changes in the state of the chromatin in human cells // Naturwissenschaften.– 1999.– Vol.86.– P.450-453.

Shckorbatov Y. The state of chromatin as an integrative indicator of cell stress // New Developments in Chromatin Research / Eds. N.M.Simpson and V.J.Stewart, 2012.– Chapter 6.– P.123-144.

Simko M. Extremely low frequency electromagnetic fields as effectors of cellular responses in vitro: possible immune cell activation / M.Simko, M.O.Mattsson // J. of Cellular Biochemistry.– 2004.– Vol.93.– P.83-92.

Szmigielski S. Reaction of the immune system to low-level RF/MW exposures // Science of the Total Environment.– 2013.– Vol. 454-455.– P.393-400.

Yao K. Electromagnetic noise inhibits radiofrequency radiation-induced DNA damage and reactive oxygen species increase in human lens epithelial cells / K.Yao, W.Wu, K.Wang et al. // Mol. Vis.– 2008.– Vol.14.– P.964-969.

Published
2015-10-07
How to Cite
Кузнецов, К. А., Мирошник, Д. Б., Пасюга, В. Н., Иванченко, Д. Д., Ключевская, О. Ю., Стойка, Р. С., & Шкорбатов, Ю. Г. (2015). Effect of low intensity microwave radiation and magnetic field on the state of chromatin in cell nuclei and contents of regulatory protein cyclin А in cells. Photobiology and Photomedicine, 11(1, 2), 64-69. Retrieved from https://periodicals.karazin.ua/photomedicine/article/view/4079
Section
Photobiology and experimental photomedicine