Low intensity laser effects on factors of congenital immunity and cytokine balance in secretions of female reproductive system affected with chlamydia infection

doi:10.26565/2076-0612-2019-26-04

О. А. Gizinger State Research Center for Laser Medicine after O.K. Skobelkina of the Federal Medical Biological Agency http://orcid.org/0000-0001-9302-0155
V. I. Karandashov State Research Center for Laser Medicine after O.K. Skobelkina of the Federal Medical Biological Agency http://orcid.org/0000-0002-0026-8862

DOI: https://doi.org/10.26565/2076-0612-2019-26-04

Keywords: laser radiation, Chlamydia trachomatis, neutrophilic granulocyte, immunity, cytokine

Abstract

Therefore, we defined the objective of our research: to study the factors of uterine cervix tissue im- munity for women with Chlamydia infection and to estimate the effect of low-intensity laser therapy (LILT) on these factors due to a wavelength of 632 nm and a power of 30 mW.

Materials and methods. Clinical immunologic studies of cervical secretion were carried out on 184 women of reproductive age 25,9 ± 4,22 in 2016–2018. Chlamydia infection Chlamydia trachomatis was found by the methods of real-time polymerase chain reaction (Rt-PCR) tests. Code by Classifier of the International Standard Classification of Diseases and Related Health Problems 10th Revision is А 56.0, Chlamydial infections of the lower sections of the urogenital tract. Urethra and canalis cervicis uteri scrapings were used as the material for detecting C. trachomatis. All patients with chlamydial infection of the lower part of the urogenital system were divided into two groups: a group of 100 women who were treated by using the local influence of the low-intensity laser of 632 nm as a part of complex treatment, the comparison group (basic treatment) consisted of 84 women who took azithromycin of 1 g once a day for the 1st, 5th, 10th days of treatment. 50 healthy women without any pathology and infections of the genitourinary system made the control group. A system of cytokines was studied by interleukin's level IL-8. IL-1 α, IL-1 β, TNF-α and IFN-γ content in cervical mucus.

Results Course. The results of the study showed a high clinical and immunological efficacy of treating chlamydial cervicitis using a low intensity laser with a wavelength of 632 nm. An imbalance of cellular factors of innate immunity revealed before starting a therapy: a decrease in the oxygen-dependent metabolism of neutrophilic granulocytes of the cervical secretion

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