The effect of Lipid Peroxidation and ionizing irradiation on platelet disaggregation caused by polypeptide antibiotic gramicidi S
Keywords:
platelets, gramicidin S, aggregation, disaggregation, lipid peroxidation, ionizing irradiation, temperatureAbstract
Turbidimetric technique to study the interaction of gramicidin S with human blood platelets has been used aiming at elucidating the mechanism of cyclic polypeptide antibiotic binding with cell membranes. We have examined the gramicidin S induced disaggregation of native platelets and ones γ-irradiated with doses 10-250 Gy; effect of membrane lipid peroxidation and antioxidants on the perameters of platelet disaggregation caused by gramicidin S was studied as well at different temperatures in the range of 8-370С. It was shown that loosening of lipids in the platelet membrane by oxidation products facilitates gramicidin S incorporation into membrane, while α-tocopherol depending on the observation temperature may both disorder and consolidate cell membrane. In vitro γ-irradiation of platelets with doses 3-5 Gy causes maximal loose of cell membrane as assessed by the rate and extent of gramicidin S induced disaggregation. Conclusion was made that the packaging density of lipids in the bilayer plays a key role in the gramicidin S incorporation into the cell membrane. Outcomes of this study allowed us to assume application of the gramicidin S as a probe sensitive for the phase state of membrane lipids in biological membranes.
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