Competitive binding of chlorophyllin and proflavine to DNA

Authors

  • A. A. Herus Институт радиофизики и электроники НАН Украины имени А.Я. Усикова, ул. Ак. Проскуры, 12, Харьков, 61085, Украина Author
  • N. A. Gladkovskaya Институт радиофизики и электроники НАН Украины имени А.Я. Усикова, ул. Ак. Проскуры, 12, Харьков, 61085, Украина Author
  • E. G. Bereznyak Институт радиофизики и электроники НАН Украины имени А.Я. Усикова, ул. Ак. Проскуры, 12, Харьков, 61085, Украина Author
  • E. V. Dukhopelnikov Институт радиофизики и электроники НАН Украины имени А.Я. Усикова, ул. Ак. Проскуры, 12, Харьков, 61085, Украина Author

DOI:

https://doi.org/10.26565/2075-3810-2016-36-06

Keywords:

DNA, chlorophyllin, proflavine, competitive binding, protector mechanism, interceptor mechanism, spectrophotometry

Abstract

The effect of chlorophyllin (Chln) on the DNA-proflavine (Pf) interactions has been investigated by spectrophotometry. The absorption spectra were recorded at different DNA-to-ligand molar ratios. Decomposition of the spectra of the Chln-Pf, Pf-DNA and Chln-Pf-DNA mixtures was performed by the principal component analysis, allowing determining the concentrations of the absorbing particles in solutions. The results indicated that chlorophyllin did not interact with DNA. The Chln-Pf heteroassociation constant (K=9×104 М) was calculated using Scatchard isotherm analysis. Furthermore, Chln showed only the interceptor properties in the ternary Chln-Pf-DNA system. Next, spectrophotometric studies of binary and ternary systems were carried out under heating of the solutions from 20 to 90°C. Temperature dependences of the particle concentrations were obtained for Chln-Pf-DNA system. It appeared that Chln interceptor properties increased with the temperature. This is due to the fact that Pf-DNA binding constant decreased under heating, while Chln-Pf heteroassociation constant did not vary significantly with the temperature.

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Issue

Vol. 2 No. 36 (2016): Biophysical Bulletin

Section

Molecular biophysics

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How to Cite

Competitive binding of chlorophyllin and proflavine to DNA. (2016). Biophysical Bulletin, 2(36), 43-50. https://doi.org/10.26565/2075-3810-2016-36-06

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