Quercitin influence on platelet aggregation in blood of insulinreistant female rats with estrogen deficiency

Abstract

Insulin resistance and hyperinsulinemia belong to specific characteristics not only of sugar diabetes 2 and obesity, but present in hyperlipidemia, atherosclerosis, and aging processes. Pharmacotherapy of metabolic syndrome must have a tendency of increased tissue sensitivity to insulin because insulin resistance is one of the main components of the syndrome. At the present day, there are investigations of the pharmacological agents, what belongs to flavonoid in particular, with the aim of using it in sugar diabetes 2 and metabolic syndrome treating. Quercitin, as representative of flavonoids, attracts attention as a potential pharmacological agent. The studying of drugs was realized in experimental disease models in rats. Chronicle saccharose introduction to ovariectomized rats promotes further evolution of insulin resistance, hypotony, hyperglicerinemy, oxidative stress, i.e. allows recreating common signs of postmenopausal human metabolic syndrome, which was used in this work to create syndrome model. For investigation were used Wister rats with weight in the range of 290-300 g. Bodyweight increase, uterus weight increase, level of platelet aggregation and it's speed, the dielectric permittivity of plasma full of platelets of rats blood, were taken as indicators of influence characteristics of quercitin on the disease. The level and speed of aggregation were measured by the photo electro colorimetry method. Real (ε') part of complex dielectric permittivity of plasma, full of platelets, was fixed by UHF-dielectrometer at 9.2 GHz. The result of the experiment was defined, that ovariectomy even in the standard diet leads to rising in the speed of ADP-induced platelet aggregation. Also in the presence of insulin resistance, there is a decrease of dielectric permeability of PFP, i.e. a result of increasing platelet concentration in blood. Using the investigated drug in 10 mg/kg dose was not correlating with speed or level of ADP-induced platelet aggregation. At the same time, individuals, who take drugs in 50 mg/kg dose, have shown the normalizing of these indexes.