BINDING ENERGY DIFFERENTIATION OF WATER MOLECULES IN DNA HYDRATION SHELL

  • K. M. Virnik Biophysics Division, Institute of Radiophysics andElectronics, Ak. Proskura St. 12, Kharkov 310085, Ukraine
  • M. E. Tolstorukov Chair of Molecular and Applied Biophysics, Radiophysics Department, Kharkov State University, SvobodySq. 4, Kharkov 310077, Ukraine
Keywords: DNA, conformations, hydration, sorption, binding energy

Abstract

An energetics study of water molecule binding to a DNA matrix has been performed. Bound water molecules have been differentiated according to their binding energies, the water molecules being supposed to participate in three types of sorption (hydration): sorption by Langmuir's binding centers , sorption by Henry's ones and multilayer sorption. It has been found that waters of the different sorption types take part in stabilizing a certain DNA conformation to a different degree. Consideration of the obtained values of free binding energies within each sorption type has shown that waters bound to Langmuir's centers mainly advantage A-DNA stabilization while ones bound to Henry's centers facilitate B-DNA stabilization. The water molecules bound in multilayer are more important for stabilization of both A- and B-form in comparison with unordered state, but largely for B-form. This is due to occurring the basic formation of multilayer part of hydration surroundings at high water content in DNA.

Downloads

Download data is not yet available.

References

1. Maleev V. Ya., Semenov M. A., Gasan A. I., Kashpur V. A.// Biophysics. - 1993. - V.38. -P.789-811.


2. Saenger W. Principles of the Structural Organization of Nucleic Acids. -M.: Mir, 1987. -584pp. (In Russian.)


3. Saenger W. //Ann. Rev. Biophys. Biophys. Chem.- 1987,- 16.-P. 93-114.

4. Gasan A. I., Maleev V. Ya., Semenov M. A. //Studia Biophys. - 1990. - V.130. -P.171-178.

5. Semenov M. A, Starikov E. B., Bolbukh T. V. // Stud. Biophys. - 1988. - V. 123. - P.217-224.


6. Tolstorukov M.E., Gatash S.V., Maleev V. Ya. // Vestnik problem biologii i meditsiny. - 1998. - No. 16. - P.6-12. (In Russian.)

7. Falk M., Hartman K. A., Lord R. C. // J. Am. Chem. Soc- 1963.- V.85.- P. 387-391.

8. D'Arcy R.L., Watt J.C. // Trans. Faraday Soc. - 1970. - V.66. - P. 1236-1245.


9. Gascoyne P.R.C., Pethig R. //J. Chem. Soc. Faraday Trans. - 1977. - V.1. - P. 171-180.

10. Bolbukh T. V., Semenov M. A., Starikov E. B., Maleev V. Ya. // Proc. of Third Europ. Conf. on the Spectr. of Biol. Mol. - Bolonya (Italy). - 1989. -P. 185-188.
Cited
How to Cite
Virnik, K. M., & Tolstorukov, M. E. (1). BINDING ENERGY DIFFERENTIATION OF WATER MOLECULES IN DNA HYDRATION SHELL. Biophysical Bulletin, 2(2). Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/2447
Issue
Vol. 2 No. 2 (1998): Біофізичний вісник
Section
Molecular biophysics

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).