• K. M. Virnik Biophysics Division, Institute of Radiophysics andElectronics, Ak. Proskura St. 12, Kharkov 310085, Ukraine
  • M. E. Tolstorukov Chair of Molecular and Applied Biophysics, Radiophysics Department, Kharkov State University, SvobodySq. 4, Kharkov 310077, Ukraine
Ключові слова: DNA, conformations, hydration, sorption, binding energy


An energetics study of water molecule binding to a DNA matrix has been performed. Bound water molecules have been differentiated according to their binding energies, the water molecules being supposed to participate in three types of sorption (hydration): sorption by Langmuir's binding centers , sorption by Henry's ones and multilayer sorption. It has been found that waters of the different sorption types take part in stabilizing a certain DNA conformation to a different degree. Consideration of the obtained values of free binding energies within each sorption type has shown that waters bound to Langmuir's centers mainly advantage A-DNA stabilization while ones bound to Henry's centers facilitate B-DNA stabilization. The water molecules bound in multilayer are more important for stabilization of both A- and B-form in comparison with unordered state, but largely for B-form. This is due to occurring the basic formation of multilayer part of hydration surroundings at high water content in DNA.





1. Maleev V. Ya., Semenov M. A., Gasan A. I., Kashpur V. A.// Biophysics. - 1993. - V.38. -P.789-811.

2. Saenger W. Principles of the Structural Organization of Nucleic Acids. -M.: Mir, 1987. -584pp. (In Russian.)

3. Saenger W. //Ann. Rev. Biophys. Biophys. Chem.- 1987,- 16.-P. 93-114.

4. Gasan A. I., Maleev V. Ya., Semenov M. A. //Studia Biophys. - 1990. - V.130. -P.171-178.

5. Semenov M. A, Starikov E. B., Bolbukh T. V. // Stud. Biophys. - 1988. - V. 123. - P.217-224.

6. Tolstorukov M.E., Gatash S.V., Maleev V. Ya. // Vestnik problem biologii i meditsiny. - 1998. - No. 16. - P.6-12. (In Russian.)

7. Falk M., Hartman K. A., Lord R. C. // J. Am. Chem. Soc- 1963.- V.85.- P. 387-391.

8. D'Arcy R.L., Watt J.C. // Trans. Faraday Soc. - 1970. - V.66. - P. 1236-1245.

9. Gascoyne P.R.C., Pethig R. //J. Chem. Soc. Faraday Trans. - 1977. - V.1. - P. 171-180.

10. Bolbukh T. V., Semenov M. A., Starikov E. B., Maleev V. Ya. // Proc. of Third Europ. Conf. on the Spectr. of Biol. Mol. - Bolonya (Italy). - 1989. -P. 185-188.
Як цитувати
Virnik, K. M., & Tolstorukov, M. E. (1). BINDING ENERGY DIFFERENTIATION OF WATER MOLECULES IN DNA HYDRATION SHELL. Біофізичний вісник, 2(2). вилучено із
Молекулярна біофізика