Species specificity of morphological changes of erythrocytes in sucrose medium
Abstract
The dynamics of shape changes of human, rat, and chicken erythrocytes upon placing them into sucrose media with low chloride content and effect of anion transport inhibitors DIDS, SITS, and DNDS, as well as albumin, chlorpromazine, SDS, and CTAB, was studied. It was shown that human and rat erythrocytes responded similarly to the changes in the ionic environment demonstrating triphasic shape response, which is significantly different from those of chicken erythrocyte where only week stomatocytosis was observed. DIDS and SITS acted similarly regardless of human or rat cells were concerned. SDS also caused similar shape changes in these erythrocytes although the effect was significantly different from those of DIDS and SITS. In all other cases, the impact of various drugs differs from each other and the same agent affects various types of cells in a different manner. The action of agents depends strongly on whether they are initially present in the media or were added to the media 150 s after the cells. Investigation of shape dynamics by means of a non-invasive method of light scattering paralleled by optical microscopy revealed that chicken erythrocytes are less prone to morphological changes in sucrose medium and in contrast to human and rat cells did not transform into spherical forms in the absence as well as in the presence of agents. It was found that rat erythrocytes are transformed into stomatocytes both in the presence and absence of agents whereas human cells are able to form schistocytes in some cases. The data obtained demonstrate that the action of drugs on shape transformation in sucrose media is a complex phenomenon that is strongly dependent on the type of erythrocytes and the chemical nature of drugs.
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