Mg2+ ion effect on conformational equilibrium of poly I-poly A-poly I triple helix in solution

  • V. A. Sorokin B.I. Verkin Institute for Low Temperature Physics and Engineering, NAS of Ukraine
  • V. A. Valeev B.I. Verkin Institute for Low Temperature Physics and Engineering, NAS of Ukraine
  • E. A. Andrus B.I. Verkin Institute for Low Temperature Physics and Engineering, NAS of Ukraine
  • G. O. Gladchenko B.I. Verkin Institute for Low Temperature Physics and Engineering, NAS of Ukraine
  • Yu. P. Blagoi B.I. Verkin Institute for Low Temperature Physics and Engineering, NAS of Ukraine
Keywords: polynucleotides, thermostability, metal ions

Abstract

Differential UV spectroscopy was used to study the temperature dependence of the conformational equilibrium in polyI-polyA-polyI (A2I) aqueous solutions containing Na+ (0,1M) and Mg2+ (10-5±0,005M) ions. Heating-induced disruption of the A2I triple helix is the A2I—>A+I+I transition (the 3—>1 transition) in the whole range of concentrations of these ions. The rise of this transition temperature with the increase of Mg2+ ion content is described satisfactorily with "ligands" theory. This fact permitted to evaluate the constant of Mg2+ ion binding to A2I (K=1250 M-1 for the null binding degree). An analytical expression has been obtained, stating a relation between values of constants of the magnesium binding to triple (K3) - and single-stranded (K1) polynucleotides and values of the linear density of charges on these polynucleotides. These values differ slightly that is conditioned with similarity of A2I and A2U structures.

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Author Biographies

V. A. Sorokin, B.I. Verkin Institute for Low Temperature Physics and Engineering, NAS of Ukraine

47 Lenin Ave., Kharkov 61103, Ukraine

V. A. Valeev, B.I. Verkin Institute for Low Temperature Physics and Engineering, NAS of Ukraine

 47 Lenin Ave., Kharkov 61103, Ukraine

E. A. Andrus, B.I. Verkin Institute for Low Temperature Physics and Engineering, NAS of Ukraine

 47 Lenin Ave., Kharkov 61103, Ukraine

G. O. Gladchenko, B.I. Verkin Institute for Low Temperature Physics and Engineering, NAS of Ukraine

47 Lenin Ave., Kharkov 61103, Ukraine

Yu. P. Blagoi, B.I. Verkin Institute for Low Temperature Physics and Engineering, NAS of Ukraine

47 Lenin Ave., Kharkov 61103, Ukraine

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Published
2018-06-25
Cited
How to Cite
Sorokin, V. A., Valeev, V. A., Andrus, E. A., Gladchenko, G. O., & Blagoi, Y. P. (2018). Mg2+ ion effect on conformational equilibrium of poly I-poly A-poly I triple helix in solution. Biophysical Bulletin, 2(13), 30-37. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/12369
Issue
Vol. 2 No. 13 (2003): Біофізичний вісник
Section
Molecular biophysics

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