The influence of media composition on the growth rate and cultural-morphological characteristics of Cladobotryum dendroides (Bull.) W. Gams & Hooz. strains
Abstract
The influence of media composition on mycelium growth rate and cultural-morphological characteristics of 5 strains of fungicolous fungus Cladobotryum dendroides (Bull.) W. Gams & Hooz. (teleomorph of Hypomyces rosellus (Alb. & Schwein.) Tul. & C. Tul.). is considered. Pure cultures of different strains of Cladobotryum dendroides were isolated from carpophores of Agaricus bisporus (J.E. Lange) Imbach, which were collected in 5 different industrial mushroom farms of Ukraine. We studied the dependence of the growth rate of Cladobotryum dendroides mycelium and changes of its cultural and morphological characteristics on media composition – Chapek agar, potato-glucose agar, mushroom agar, in Petri dishes at temperature 18±1ºС. The radius of colonies was measured every day in two mutually perpendicular directions in order to estimate the radial growth rate (mm/day). The growth rate of the studied strains was maximal on the potato-glucose agar, strain 2 grew on the potato-glucose agar and Chapek agar with identical rate. Growth rate of strain 2 on the potato-glucose agar was higher than on the mushroom agar by 23%, and for strain 5 this difference was 80%. There was a difference of morphological features of strains colonies depending on composition of media: on the potato-glucose agar from the inoculum to the edge of the colony there were observed concentric ring-like formations, the edge of colony was distinctly outlined; on the Chapek agar radial strip-like formations of different expressiveness were from the inoculum and to the edge, the edge of colony was inexpressive; on the mushroom agar colonies had relatively large, pink inoculum of protuberant form, around it there was a white disk, from the middle and to the edge the colony was transparent, its edge was poorly outlined.
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References
Билай В.И. Методы экспериментальной микологии: Справочник. – Киев: Наук. думка, 1982. – 583с. /Bilay V.I. Metody eksperimental'noy mikologii: Spravochnik. – Kiyev: Nauk. dumka, 1982. – 583s./
Наумов Н.А. Методы микологических и фитопатологических исследований. – М.; Л.: Сельхозгиз, 1937. – 272с. /Naumov N.A. Metody mikologicheskikh i fitopatologicheskikh issledovaniy. – M.; L.: Sel'khozgiz, 1937. – 272s./
Рудаков О.Л. Микофильные грибы, их биология и практическое значение. – М.: Наука, 1981. – 160с. /Rudakov O.L. Mikofil'nyye griby, ikh biologiya i prakticheskoye znacheniye. – M: Nauka, 1981. – 160s./
Chang-Gi Back, Chang-Yun Lee, Geon-Sik Seo, Hee-Young Jung Characterization of species of Cladobotryum which cause cobweb disease in edible mushrooms grown in Korea // Mycobiology. – 2012. – Vol.40 (3). – P. 189–194.
McKay G.J., Egan D., Morris E., Brown A.E. Genetic and morphological characterization of Cladobotryum species causing cobweb disease of mushrooms // Applied and Environmental Microbiology. – 1999. – Vol.65. – P. 606–610.
Potočnik I., Rekanović E., Milijašević S. et al. Morphological and pathogenic characteristics of the fungus Cladobotryum dendroides, the causal agent of cobweb disease of the cultivated mushroom Agaricus bisporus in Serbia // Pestic. fitomed (Beograd). – 2008. – Vol.23. – P. 175–181.
Rogerson C.T., Samuels G.J. Agaricicolous species of Hypomyces // Mycologia. – 1994. – Vol.86 (6). – P. 839–866.
Rogerson C.T., Samuels G.J. Polyporicolous species of hypomyces // Mycologia. // The New York Botanical Garden, Bronx, 1993. – Vol.85 (2). – P. 231–272.
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