Influence of genotype and bacterization on growth, development, and soluble carbohydrate content in soybean E-genes isogenic lines

Keywords: Glycine max (L.) Merr., Bradyrhizobium japonicum, E-series genes, isogenic lines, bacterization, phenophases, biomass, mono- and oligosaccharides,


Photoperiod, which regulates the duration of vegetative and generative development, and the plant-microorganism interaction, which influences the metabolic status of plant organisms, are important factors in the regulating plant growth and development. The aim of the study was to determine the influence of Glycine max (L.) Merr. genotype and seed pre-bacterization with a virulent and active strain of Bradyrhizobium japonicum 634b on the plant growth and development, and on the soluble carbohydrate content in leaves of isogenic by E-genes lines under field conditions. Nearly isogenic lines (NILs) of soybean, in which the E1, E2, and E3 genes are located at different allelic loci, were used. Sterile seeds were pretreated with distilled water (control) and Bradyrhizobium japonicum 634b cell suspension (experiment). Plants were grown under natural long-day conditions (16 hours). The growth and development of the soybean were evaluated by phenological observations, morphometric indicators fixed at the V3 and V5 developmental stages, relative growth rate (RGR), and the content of soluble sugars ‒ mono- and oligosaccharides. The effect of the factors studied (genotype, bacterization, and their interaction) was calculated. The results of the experiment and the calculation of the effect of the factor showed that the isoline genotype has the greatest effect on seed germination, phenological development of the plant and duration of the VE-R1 phase, growth of the root system in the V3 and V5 phases, and the content of monosaccharides involved in forming the plant-microorganism interaction. The effect of bacterization is most evident in the RGR, shoot development, and the oligosaccharide content of the leaves of NILs in the V3 and V5 phases. Among the isolines studied, L 80-5879, which has the E1 gene (flowering repressor) in a dominant state, was characterized by minimal sensitivity to bacterization. It was found that bacterization and genotype interaction didn't influence the VE-R1 duration stage and the shoot and root length. The results obtained therefore prove that the E-series genes, which determine the photoperiodic sensitivity of soya beans, can also be indirectly involved in establishing plant-microorganism interactions.


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Author Biographies

D. Hlushach, V.N. Karazin Kharkiv National University

Svobody Sq., 4, Kharkiv, Ukraine, 61022,

V. Zhmurko, V.N. Karazin Kharkiv National University

Svobody Sq., 4, Kharkiv, Ukraine, 61022,

O. Avksentieva, V.N. Karazin Kharkiv National University

Svobody Sq., 4, Kharkiv, Ukraine, 61022,


Avksentieva O.O., Zhmurko V.V., Shchogolev A.S., Yukhno Yu.Yu. (2018). Physiology and biochemistry of plants – small practical course: educational and methodological manual. V.N. Karazin Kharkiv National University. 152 p.

Atramentova L.O., Utevska O.M. (2008). Statistical methods in biology. Gorlivka: Likhtar. 248 p.

Hlushach D., Zhmurko V., Avksentyeva O. (2022). Influence of bacterization on protein content in leaves of soybean isogenic lines by genes of photoperiodic sensitivity control in field. InterConf, 23(117), 197–208.

Kozar S.F., Skorik V.V., Usmanova T.O., Evtushenko T.A. (2012). The influence of stabilizators on the growth, viability and functional activity of Bradyrhizobium japonicum. Agricultural Microbiology, 15, 58–70.

Melnykova N.M., Kots S.Ya. (2019). Effect of goat’s-rue rhizobia on the formation and functioning of the soybean – Bradyrhizobium japonicum 634b symbiosis. Agricultural Microbiology, 29, 29–36.

Okhrymovych O., Chebotar S., Chebotar G., Zharikova D. (2020). Molecular structure of soybean E-genes and their functional mutations. Visnyk of Lviv University. Biological series, 82, 3–13.

Popova Y.V., Zhmurko V.V. (2014). The nitrogen fixing activity of the soybean Glycine max (L.) Merr. near-isogenic by E-genes lines under different photoperiod. The Journal of V.N.Karazin Kharkiv National University. Series "Biology", 23 (1129), 21–28.

Postoi V.V. (2021). Obtaining transfer factor from lymphocytes of bovine colostrum and its adaptogenic properties. Abstract of dissertation … Candidate of Veterinary Sciences: 16.00.03 «Veterinary microbiology, epizootology, infectious diseases and immunology». Kyiv. 20 p.

Andrés F., Kinoshita A., Kalluri N. et al. (2020). The sugar transporter SWEET10 acts downstream of FLOWERING LOCUS T during floral transition of Arabidopsis thaliana. BMC Plant Biology, 20(1), 53.

Backer R., Rokem J.S., Ilangumaran G. et al. (2018). Plant growth-promoting rhizobacteria: context, mechanisms of action, and roadmap to commercialization of biostimulants for sustainable agriculture. Frontiers in Plant Science, 9, 1473.

Chen F., Li Y., Li X. et al. (2021). Ectopic expression of the Arabidopsis florigen gene FLOWERING LOCUS T in seeds enhances seed dormancy via the GA and DOG1 pathways. Plant J., 107(3), 909–924.

Chitra K. (2014). Influence of PGPR on pigment concentration on Glycine max (L). Merr. International Journal of Current Microbiology and Applied Sciences, 3, 1110–1115.

Dechaine J.M., Gardner G., Weinig C. (2009). Phytochromes differentially regulate seed germination responses to light quality and temperature cues during seed maturation. Plant, Cell & Environment, 32(10), 1297–1309.

Fagorzi C., Bacci G., Huang R. et al. (2021). Nonadditive transcriptomic signatures of genotype-by-genotype interactions during the initiation of plant-rhizobium symbiosis. MSystems, 6(1), e00974–20.

Hayat R., Ahmed I., Sheirdil R.A. (2012). An overview of Plant Growth Promoting Rhizobacteria (PGPR) for sustainable agriculture. Crop Production for Agricultural Improvement, 557–579.

Hennion N., Durand M., Vriet C. et al. (2019). Sugars en route to the roots. Transport, metabolism and storage within plant roots and towards microorganisms of the rhizosphere. Physiologia Plantarum, 165(1), 44–57.

Hunt R. (2017). Growth analysis, individual plants. Encyclopedia of Applied Plant Sciences, 17, 421–429.

Lepetit M., Brouquisse R. (2023). Control of the rhizobium-legume symbiosis by the plant nitrogen demand is tightly integrated at the whole plant level and requires inter-organ systemic signaling. Frontiers in Plant Science, 14, 1114840.

Liu L., Song W., Wang L. et al. (2020). Allele combinations of maturity genes E1-E4 affect adaptation of soybean to diverse geographic regions and farming systems in China. PLOS ONE, 15(7),

Mishra P., Panigrahi K.C. (2015). GIGANTEA – an emerging story. Frontiers in Plant Science, 6(8). 1–15.

Roeber V.M., Schmülling T., Cortleven A. (2022). The photoperiod: handling and causing stress in plants. Frontiers in Plant Science, 12, 781988.

Schogolev A.S., Raievska I.M. (2021). Role of nitrogen deficiency on growth and development near isogenic by E genes lines of soybean co-inoculated with nitrogen-fixing bacteria. Regulatory Mechanisms in Biosystems, 12(2), 326–334.

Shrestha A., Zhong S., Therrien J. et al. (2021). Lotus japonicus nuclear factor YA1, a nodule emergence stage-specific regulator of auxin signalling. The New Phytologist, 229(3), 1535–1552.

Taniguchi T., Murayama N., Ario N. et al. (2020). Photoperiod sensing of leaf regulates pod setting in soybean (Glycine max (L.) Merr.). Plant Production Science, 23(3), 360–365.

Tasma I.M., Shoemaker R.C. (2003). Mapping flowering time gene homologs in soybean and their association with maturity loci. Crop Science, 43(1), 319–328.

Tasma I.M., Lorenzen L.L., Green D.E., Shoemaker R.C. (2001). Mapping genetic loci for flowering time, maturity, and photoperiod insensitivity in soybean. Molecular Breeding, 8(1), 25–35.

Tsubokura Y., Watanabe S., Xia Z. et al. (2014). Natural variation in the genes responsible for maturity loci E1, E2, E3 and E4 in soybean. Annals of Botany, 113(3), 429–441.

Wahl V., Ponnu J., Schlereth A. et al. (2013). Regulation of flowering by trehalose-6-phosphate signaling in Arabidopsis thaliana. Science, 339(6120), 704–707.

Wang T., Guo J., Peng Y. et al. (2021). Light-induced mobile factors from shoots regulate rhizobium-triggered soybean root nodulation. Science, 374(6563), 65–71.

Xia Z., Watanabe S., Yamada T. et al. (2012). Positional cloning and characterization reveal the molecular basis for soybean maturity locus E1 that regulates photoperiodic flowering. Proceedings of the National Academy of Sciences of the United States of America, 109(32), 2155–2164.

Xu M., Yamagishi N., Zhao C. et al. (2015). The soybean-specific maturity gene E1 family of floral repressors controls night-break responses through down-regulation of FLOWERING LOCUS T orthologs. Plant Physiology, 168(4), 1735–1746.

Yang Q., Yang Y., Xu R. et al. (2019). Genetic analysis and mapping of QTLs for soybean biological nitrogen fixation traits under varied field conditions. Frontiers in Plant Science, 10(75), 1–11.

Zhao C., Takeshima R., Zhu J. et al. (2016). A recessive allele for delayed flowering at the soybean maturity locus E9 is a leaky allele of FT2a, a FLOWERING LOCUS T ortholog. BMC Plant Biol, 16(20), 1–15.

How to Cite
Hlushach, D., Zhmurko, V., & Avksentieva, O. (2023). Influence of genotype and bacterization on growth, development, and soluble carbohydrate content in soybean E-genes isogenic lines . The Journal of V.N.Karazin Kharkiv National University. Series «Biology», 40, 59-70.