Features of colonization of the phytosphere of wheat seedlings by introduced strains of Escherichia coli
Abstract
The paper presents the results of the study of the ability of introduced Escherichia coli strains to colonize different niches of the phytosphere – the rhizosphere, endosphere, and phylosphere of Mersia winter soft wheat seedlings and to influence the plant growth response under these conditions. The ATCC 8739 and clinical E. coli strains were used as the study material, as well as an isolate from the wheat agrocenosis soil. The vegetation experiments were carried out in the factorial chamber of the Department of Plant Physiology and Biochemistry of Plants and Microorganisms of V.N.Karazin Kharkiv National University. The experimental plants were inoculated with suspensions of E. coli strains and soil isolate by watering them in vegetation vessels where seedlings were grown for 10 days. In parallel by the method of successive washes, we analyzed the dynamics of the number of E. coli CFU in the rhizome, endo- and phyllosphere of seedlings. After completion of the experiment, the growth response was analyzed by linear growth and by the integral index of growth and biosynthetic processes – biomass accumulation. The results of experiments show that inoculation with ATCC 8739 and clinical strains of E. coli reduced the germination of wheat seeds and inhibited the growth response. Inoculation with E. coli soil isolate has virtually no effect on seed germination, linear growth, and biomass accumulation by Mersia seedlings. It was found that the number of E. coli bacteria in the phytosphere of wheat plants depended on the type of inoculated strain and differs in different parts of the seedlings phytosphere. The maximum number of E. coli CFU in the phytosphere was detected at the inoculation of plants with the clinical strain, three times less – at exposure to the soil isolate, almost 6 times less – at the inoculation with the standard strain. Differences in the degree of colonization of different parts of the phytosphere were detected at the inoculation of the test seedlings with different strains and soil isolates of E. coli: in the control variant and under the influence of ATCC 8739, the distribution between the rhizo- and endosphere is approximately the same. When the plants were inoculated with the clinical strain, endosphere was colonized, when using the soil isolate – the rhizosphere. In the phyllosphere of the experimental seedlings, only cells of the clinical strain were found in a small number, which indicates its increased adhesive properties. Plant-microbial relationships and the ability of conditionally pathogenic E. coli bacteria to colonize different areas of the plant organism and to use wheat seedlings as an alternative host are discussed.
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References
Alekseyenko A.L. (2010). Features of the interaction of opportunistic enterobacteria with plants. Abstract of the thesis for the Degree of the Candidate of Biological Sciences. Irkutsk. 22 p. (In Russian)
Ivanova E.I., Popkova S.M., Dzhioyev Y.P., Rakova E.B. (2013). Detection of pathogenicity genes encoding ability to toxigenesis in Escherichia coli isolated from the children intestinal biotope. Acta Biomedica Scientifica, 2(2), 111–114. (In Russian)
Lakin G.F. (1973). Biometrics. Moscow: Vysshaya shkola Publ. 343 p. (In Russian)
Markova Yu.A., Turskaya A.L. (2012). Plants as an ecological niche of bacteria pathogenic for humans. Scientific Journal Cube AU, 84(10), 1–15. (In Russian)
Moshynets O.V., Kosakivska I.V. (2010a). Phytosphere ecology: plant-microbial interactions. 1. Structure-functional characteristic of rhizo-, endo- and phyllosphere. The Bulletin of Kharkiv National Agrarian University: Series Biology, 2, 19–35. (In Ukrainian)
Moshynets O.V., Kosakivska I.V. (2010b). Phytosphere ecology: plant-microbial interactions. 2. Phytosphere as a niche for plant-microbial interactions. Functional microbial activity and its influence on plants. The Bulletin of Kharkiv National Agrarian University: Series Biology, 3, 6–22.](In Ukrainian)
Organic production and food safety. (2013). Zhytomyr: Polissya. 492 р. (In Ukrainian)
Pozdeyev O.K., Fedorov R.V. (2007). Enterobacteria: a guide for doctors. Moscow: GEOTAR-Media. 720 p. (In Russian)
Workshop on microbiology (2005). / Ed. A.I.Netrusov. Moscow: Publishing Center "Academy". 602 p. (In Russian)
Rybalka O.I. (2011). Wheat quality and its improvement. Kyiv: Logos. 496 p. (In Ukrainian)
Solovieva E., Aleschenkova Z. (2018). Isolation of endophytic phosphate-solubilizing bacteria that colonize the internal tissues of soy and winter wheat. Žmogaus ir gamtos sauga, 208–210. (In Russian)
Khakimova L.R., Lavina A.M., Serbaeva E.R. et al. (2017). The role of bacterial adhesins and other cell components in the initial stages of plant-microbial interactions. Biomika, 9(4), 325–339. (In Russian)
Shmidt V.M. (1984). Mathematical methods in botany. Leningrad: Publishing house of Leningrad University. 288 p. (In Russian)
Frank A.C., Saldierna Guzmán J.P., Shay J.E. (2017). Transmission of bacterial endophytes. Microorganisms, 5(4), 70. https://doi.org/10.3390/microorganisms5040070
Eibenbergera K., Moencha D., Drissnerb D. et al. (2018). Adherence factors of enterohemorrhagic Escherichia coli O157:H7 strain Sakai influence its uptake into the roots of Valerianella locusta grown in soil. Food Microbiology, 76, 245–256. https://doi.org/10.1016/j.fm.2018.05.016
Hofmann A., Fischer D., Hartmann A., Schmid M. (2014). Colonization of plants by human pathogenic bacteria in the course of organic vegetable production. Frontiers in Microbiology, 5(191). https://doi.org/10.3389/fmicb.2014.00191
Holden N., Pritchard L., Toth I. (2009). Colonization outwith the colon: plants as an alternative environmental reservoir for human pathogenic enterobacteria. FEMS Microbiology Reviews, 33(4), 689–703. https://doi.org/10.1111/j.1574-6976.2008.00153.x
Jechalke S., Schierstaedt J., Becker M. et al. (2019). Salmonella establishment in agricultural soil and colonization of crop plants depend on soil type and plant species. Front. Microbiol., 10, 967. https://doi.org/10.3389/fmicb.2019.00967
Jo S.H., Park J.M. (2019). The dark side of organic vegetables: interactions of human enteropathogenic bacteria with plants. Plant Biotechnology Reports, 13, 105–110. https://doi.org/10.1007/s11816-019-00536-1
Karmakar K., Nath U., Nataraja K.N., Chakravortty D. (2018). Root mediated uptake of Salmonella is different from phyto-pathogen and associated with the colonization of edible organs. BMC Plant Biology, 18, 344. https://doi.org/10.1186/s12870-018-1578-9
Liu D., Cui Y., Walcott R., Chena J. (2018). Fate of Salmonella enterica and enterohemorrhagic Escherichia coli cells artificially internalized into vegetable seeds during germination. Applied and Environmental Microbiology, 84(1), e01888-17. https://doi.org/10.1128/AEM.01888-17
Merget B., Forbes K.J., Brennan F. et al. (2019). Influence of plant species, tissue type, and temperature on the capacity of shiga-toxigenic Escherichia coli to colonize, grow, and be internalized by plants. Applied and Environmental Microbiology, 85(11), e00123-19. https://doi.org/10.1128/AEM.00123-19
Moyne А.-L., Blessington T., Thomas R. et al. (2020). Conditions at the time of inoculation influence survival of attenuated Escherichia coli O157:H7 on field-inoculated lettuce. Food Microbiology, 85, 103274. https://doi.org/10.1016/j.fm.2019.103274
Murphy S., Gaffney M.T., Fanning S., Burgess C.M. (2016). Potential for transfer of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella Senftenberg from contaminated food waste derived compost and anaerobic digestate liquid to lettuce plants. Food Microbiology, 59, 7–13. https://doi.org/10.1016/j.fm.2016.04.006
Riggio G.M., Wang Q., Kniel K.E., Gibson K.E. (2019). Microgreens – а review of food safety considerations along the farm to fork continuum. International Journal of Food Microbiology, 290, 76–85. https://doi.org/10.1016/j.ijfoodmicro.2018.09.027
Wright K.M., Holden N.J. (2018). Quantification and colonization dynamics of Escherichia coli O157:H7 inoculation of microgreens species and plant growth substrates. International Journal of Food Microbiology, 273, 1–10. https://doi.org/10.1016/j.ijfoodmicro.2018.02.025
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