Cultivation of Pleurotus ostreatus (Jacq.:Fr.) Kumm. influenced by laser irradiation
Abstract
It has been studied the effect of laser irradiation on growth parameters, fruiting terms and crop capacity of Pleurotus ostreatus under solid phase cultivation on different types of substrates that include sunflower husk (SH), wheat straw (WS) and floral scales of corn ears (FSCE). According to the research carried out the best P. ostreatus mycelium growth was revealed under the cultivation on 100% FSCE, the mycelium growth on 50% FSCE was 37,7% less. The mycelium growth on 50% SH, 100% SH and 50% WS substrates was 50.5%, 50.3% and 45.0% less respectively. The least mycelium growth was recorded on 100% WS substrate. Laser irradiation nonetheless had a positive effect on the mycelium growth on the substrates under analysis. In particular, the best reaction was in response to green spectrum eradiation under the cultivation on 100% wheat straw substrate that was 71.8% better than the control. Under the cultivation on other types of substrates the mycelium growth at green spectrum eradiation increased from 23.1% to 33.7% respectively. Red and blue spectra eradiation caused only slight mycelium growth changes. Green spectrum eradiation within 10 seconds promoted the crop capacity on all the substrates from 51.5 to 80.7%, except for the substrate with SH:WS:FSCE (25:25:50%), in which the crop capacity increased the most – by 87.9%. Also 10 second green spectrum impact on the mycelium reduced the substrate fouling term and accelerated the fruiting. It has been proved that the fruiting bodies grown out of the mycelium that was under 10 second green spectrum eradiation form in greater quantity compared to non-irradiated variants. Any significant differences in fungi fruiting bodies morphology on the substrates mentioned have not been found. Thus, the research carried out allowed to distinguish the most productive substrates and the most efficient mode of P. ostreatus growth stimulation with the help of laser irradiation. The results of the research prove the expediency of laser irradiation usage while cultivating macromycete P. ostreatus.
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Бисько Н.А., Бухало А.С., Вассер С.П. и др. Высшие съедобные базидиомицеты в поверхностной и глубинной культуре. – К.: Наук. думка, 1983. – 312с. /Bysko N.A., Bukhalo A.S., Vasser S.P. et al. Higher edible basidiomycetes in superficial and deep culture. – Kyiv: Naukova Dumka, 1983. – 312p./
Бухало А.С. Высшие съедобные базидиомицеты в чистой культуре. – К.: Наукова думка, 1988. – 144с. /Bukhalo A.S. Highest edible basidiomycetes in pure culture. – Kyiv: Naukova Dumka, 1988. – 144p./
Бухало А.С., Бисько Н.А., Соломко Э.Ф. и др. Культивирование съедобных и лекарственных грибов: Практ. реком. – К., 2004. – 120с. /Bukhalo A.S., Bysko N.A., Solomko E.F. et al. Cultivation of edible and medicinal mushrooms: Practical recommendations. – Kyiv, 2004. – 120р./
Вакарчук I.О. Квантова механiка: пiдручник. – Львiв: ЛНУ iменi Iвана Франка, 2012. – 872с. /Vakarchuk I.O. Quantum mechanics: a textbook. – Lviv: Ivan Franko National University of Lviv, 2012. – 872p./
Васюра А.С. Елементи та пристрої систем управління автоматики. – Вінниця: ВДТУ, 1998. – 420с. /Vasiura A.S. Elements and devices of automation control systems. – Vinnytsa: Vinnytsa National Technical University, 1998. – 420p./
Власенко К.М. Вплив хімічного складу субстрату на показники росту, врожайності та синтез летких органічних сполук при твердофазному культивуванні Рleurotus ostreatus // Наукові доповіді НУБіП України. – 2018. – Т.2, №72. – С. 2–13. /Vlasenko K. M. Influence of substrate chemical composition on growth, yield and synthesis of volatile organic compounds in solid-phase cultivation of Pleurotus ostreatus // Naukovi dopovidi NUBiP of Ukrainе – 2018. – Vol.2, no.72. – Р. 2–13./
Дорошкевич Н.В. Вплив лазерного опромінення на продуктивність гриба Pleurotus ostreatus (Jacq.: Fr.) Kummer // Вісник Донецького університету. Сер. А. Природн. науки. – 2007. – №1. – С. 290–292. /Doroshkevich N.V. Influence of laser irradiation on Pleurotus ostreatus (Jacq.: Fr.) Kummer mushroom productivity // Visnyk of Donetsk University. Ser. A. Natural science – 2007. – No.1. – P. 290–292./
Кару Т.Й. Универсальный клеточный механизм лазерной биостимуляции: фотоактивация фермента дыхательной цепи цитохромоксидазы. Голография: фундаментальные исследования, инновационные проекты и нанотехнологии // ХХVІ школа по когерентной оптике и голографии. – 2008. – С. 156–175. /Karu T.Y. Universal cellular mechanism of laser biostimulation: photoactivation of the enzyme of the respiratory chain of cytochrome oxidase. Holography: fundamental research, innovative projects and nanotechnologies // XXVI School of Coherent Optics and Holography. – 2008. – P. 156–175./
Поединок Н.Л., Бисько Н.А., Михайлова О.Б. и др. Интенсификация технологических этапов культивирования съедобного гриба вешенки обыкновенной // Биотехнология. – 2004. – №5. – С. 64–67. /Poedynok N.L., Bysko N.A., Myhaylova O.B. et al. Intensification of the technological stages of cultivation of edible oyster mushroom // Biotechnology. – 2004. – No.5. – P. 64–67./
Поединок Н.Л., Бисько Н.А. Использование света в биотехнологии промышленного культивирования вешенки обыкновенной и шампиньона двуспорового // Достижения, проблемы и перспективы культивирования грибов. Современные технологии. Сборник научных трудов. – 2005. – С. 24–27. /Poedynok N.L., Bysko N.A. The use of light in the biotechnology of industrial cultivation of oyster mushroom and Agaricus bisporus // Achievements, problems and prospects for the cultivation of mushrooms. Modern technologies.Collection of scientific papers. – 2005. – P. 24–27./
Поединок Н.Л., Михайлова О.Б., Ходаковский В.М., Дудка И.А. Влияние на ростовую активность посевного материала культивируемых макромицетов низкоинтенсивного лазерного излучения // Мікробіологія и біотехнологія. – 2015. – Т.29, №1. – С. 77–86. /Poedynok N.L., Myhaylova O.B., Hodakovskyy V.M., Dudka I.A. Influence on growth activity of the seed material of cultivated macromycetes of low-intensity laser radiation // Microbiology and Biotechnology. – 2015. – Vol.29, no.1. – P. 77–86./
Поєдинок Н.Л. Енергоефективні системи штучного освітлення у технологіях вирощування їстівних та лікарських грибів // Наука та інновації. – 2013. – Т.9, №3. – С. 46–59. /Poyedynok N.L. Energy-efficient artificial lighting systems in edible and medicinal mushroom cultivation technologies // Science and Innovation. – 2013. – Vol.9, no.3. – P. 46–59./
Приседський Ю.Г. Пакет програм для проведення статистичної обробки результатів біологічних експериментів. – Донецьк: ДонНУ, 2005. – 84с. /Prysedskyy Yu.G. Software package for statistical processing of the results of the biological experiments – Donetsk: DonNU, 2005. – 84p./
Приседський Ю.Г. Статистична обробка результатів біологічних експериментів. – Донецьк: Кассиопея, 1999. – 210с. /Prysedskyy Yu.G. Statistical processing of the results of biological experiments. – Donetsk: Cassiopeia, 1999. – 210p./
Федотов О.В., Брусніцина О.М. Вплив джерел вуглецевого живлення на ріст і каталазну активність штаму Р-6v Pleurotus ostreatus // Проблеми екології та охорони природи техногенного регіону. – 2008. – Т.1, №8. – С. 248–253. /Fedotov O.V., Brusnitsyna O.M. The influence of carbon sources on the growth and catalase activity of strain P-6v of Pleurotus ostreatus // Problems of ecology and nature protection of technogenic region. – 2008. – Vol.1, no.8. – P. 248–253./
Bhattacharjya D.K., Paul R.K., Miah Md.N., Ahmed K.U. Comparative study on nutritional composition of oyster mushroom (Pleurotus ostreatus Fr.) cultivated on different sawdust substrates // Bioresearch Communications. – 2015. – Vol.1 (2). – P. 93–98.
Curvetto N.R., Figlas D., Devalis R., Delmastro S. Growth and productivity of different Pleurotus ostreatus strains on sunflower seed hulls supplemented with N–NH4 and/or Mn(II) // Bioresource Technology. – 2002. – Vol.84. – P. 171–176.
Сhang S.T., Miles P.G. A new look at cultivated mushrooms // Bioscience. – 1984. – Vol.34, no. 6. – P. 358–362.
Dubey S.C. Effect of different substrates and amendments on yield of Pleurotus sp. // Mycology and Plant Pathology. – 1999. – No.29. – Р. 209–216.
El Kattan M.H., Helmy Z.A., El Leithy M.A., Abdelkwai K.A. Studies on cultivation techniques and chemical composition of oyster mushrooms // Mushroom J. Tropics. – 1991. – Vol.11. – Р. 59–66.
Figlas N.D., Matute G., Curvetto N. Sunflower seed hull: its value as a broad mushroom substrate // Annals of Food Processing and Preservation. – 2016. – Vol.1 (1). – 1002.
Galagan G.V., Calvo S.E. Borkovich K.A. et al. The genome sequence of the filamentous fungus Neurospora crassa // Nature. – 2003. – Vol.422. – Р. 859–868.
Herrera–Estrella A., Horwitz B.A. Looking through the eyes of fungi: molecular genetics of photoreception // Molecular Microbiology. – 2007. – Vol.64, no.1. – Р. 5–15.
Hoa H.T., Wang C.-L., Wang C.-H. The effects of different substrates on the growth, yield, and nutritional composition of two oyster mushrooms (Pleurotus ostreatus and Pleurotus cystidiosus) // Mycobiology. – 2015. – Vol.43 (4). – P.423–434.
Kamada T., Sano H., Nakazawa T., Nakahori K. Regulation of fruiting body photomorphogenesis in Coprinopsis cinerea // Fungal Genetics and Biology. – 2010. – Vol.11. – Р. 917–921.
Karu T.J. On the molecular mechanism of therapeutic action of low–intensity laser radiation // Dokl. AN SSSR. – 1986. – Vol.29. – Р. 1245–1249.
Lelley J.I., Janben A. Interactions between supplementation, fructification–surface and productivity of the substrate of Pleurotus // Mushroom Biology and Mushroom Products. – 1993. – Vol.9. – Р. 345–349.
Nakano Y., Fujii N., Kojima M. Identification of blue-light photoresponse genes in mushroom mycelia // Bioscience, Biotechnology, and Biochemistry. – 2010. – Vol.10. – Р. 2160–2165.
Poyedinok N.L. Influence of low-intensity laser radiation on the growth and development of Hericiun erinaceus (Bull.:Fr.) Pers. and Pleurotus ostreatus (Jacq.:Fr.) Kumm. // Int. J. Med. Mushr. – 2001. – Vol.3, no.2–3. – P.199.
Poyedinok N.L., Potemkina J.V., Buchalo A.S. et al. Stimulation with low–intensity laser light of basidiospore germination and growth of monocaryotic isolates in Medicinal Mashroom Hericium erinaecus (Bull.: Fr.) Pers. (Aphyllophoromycetideae) // Int. J. Med. Mushr. – 2000. − Vol.2, no.4. – P. 339−342.
Poyedinok N.L., Buchalo A.S., Negriyko A.M. et al. The action of argon and helium-neon laser radiation on growth and fructification of culinary-medicinal mushrooms Pleurotus ostereatus (Jacq.:Fr.) Kumm., Lentinus edodes (Berk.) Singer, and Hericium erinaceus (Bull.:Fr.) Pers. // Int. J. Med. Mushr. – 2003. – Vol.5. – P. 293–299.
Purschwitz J., Muller S., Kastner C., Fischer R. Seeing the rainbow: light sensing in fungi // Current Opinion in Microbiology. – 2006. – Vol.9. – P. 566–571.
Reshetnyk K.S. Investigation the effect of laser irradiation on the growth characteristics of Pleurotus ostreatus (Jacq.) P.Kumm. // 2nd International Conference «Smart Bio». – 2018. – P.344.
Royse D.J., Zaki S.A. Yield stimulation of Pleurotus flabellatus by dual nutrient supplementation of pasteurized wheat straw // Science and Cultivation of Edible Fungi. – 1991. – P. 545–547.
Zhenzhong Yu., Reinhard F. Light sensing and responses in fungi // Nature Reviews Microbiology. – 2018. – No.17 (1). – P. 25–36.
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