ABOUT USING OF SECONDARY ION MASS SPECTROMETRY METHOD FOR RESEARCH OF BIOORGANIC OBJECTS

doi:10.26565/2312-4334-2015-4-05

V. A. Litvinov V.N. Karazin Kharkiv National University 4 Svoboda Sq., Kharkiv, 61022
V. T. Koppe V.N. Karazin Kharkiv National University 4 Svoboda Sq., Kharkiv, 61022
V. V. Bobkov V.N. Karazin Kharkiv National University 4 Svoboda Sq., Kharkiv, 61022

DOI: https://doi.org/10.26565/2312-4334-2015-4-05

Abstract

The problems of application of secondary ion mass spectrometry (SIMS) with a liquid matrix for the study of objects of organic origin, as well as some aspects of the methodology of the liquid matrix using, in particular, for minimizing the effect of ion probe on the investigated object were considered. It was shown that the use of SIMS with the liquid matrix allowed to analyze reactions in a rather complicated bioorganic systems, including intermediate products of reactions, from the behavior of secondary ions characteristic for system components. Furthermore, after the necessary calibration procedure, the method permitted to determine the concentration of components during the reaction. The results of the study of the enzymatic oxidation of glucose were presented. It was shown that the use of SIMS with a liquid matrix allowed investigating the influence of radiation on materials of organic origin. The results of the study of radiation damage of molecules of arginine amino acids, glutamic acid and tryptophan under ultraviolet irradiation were represented.

Downloads

Download data is not yet available.

Author Biography

V. V. Bobkov, V.N. Karazin Kharkiv National University 4 Svoboda Sq., Kharkiv, 61022

vbobkov@mail.ru

References

Benninghoven A., Rüdenauer F.G., Werner H.W. Secondary Ion Mass Spectrometry: Basic Concepts, Instrumental Aspects, Applications, and Trends. - New York: Wiley, 1987. - 1227 p.

Fogel’ Ya. M. Ion-ion emission - a new tool for mass-spectrmetric investigation of processes on the surface and in the bulk of solids. // International Journal of Mass Spectrometry and Ion Physics. – 1972. - Vol. 9. - P. 109-125.

Kljuev N.A., Brodskij E.S. Sovremennye metody mass-spektrometricheskogo analiza organicheskih soedinenij // Ros. Him. Zhurn. - 2002. - Т. XLVI. – No. 4. - S. 57-63.

Mak-H'ju I.A. Metody analiza poverhnosti. Pod red. Zandernі. – M.: Mir, 1979. - S. 276-336.

Cherepin V.T. Ionnyj mikrozondovyj analiz. - Kiev: Naukova Dumka, 1992. - 344 s.

Benninghoven A., Sichtermann W.K. Detection, identification and structural investigation of biologically important compounds by secondary ion mass spectrometry // Anal. Chem. - 1978. - Vol. 50. – No. 8. - Р. 1180-1184.

Kempson I.M., Martin A.L., Denman J.A., French P.W., Prestidge C.A., Barnes T.J. Detecting the Presence of Denatured Human Serum Albumin in an Adsorbed Protein Monolayer Using TOF-SIMS // Langmuir. - 2010. - Vol. 26. – No. 14. - P. 12075-12080.

Saleem M., Galla H.J. Surface view of the lateral organization of lipids and proteins in lung surfactant model systems-A ToF-SIMS approach // Biochim. Biophys. Acta. - 2010. - Vol. 1798. – No. 4. - P. 730-740.

Kim Y.P., Hong M.Y., Kim J, Oh E., Shon H.K., Moon D.W., Kim H.S., Lee T.G. Quantitative analysis of surface-immobilized protein by TOF-SIMS: effects of protein orientation and trehalose additive. // Anal Chem. - 2007. - Vol. 79. – No. 4. - P. 1377-1385.

Mahoneya C.M., Yub L., Faheya A., Gardella J.A. Jr. SIMS depth profiling of polymer blends with protein based drugs // Applied Surface Science.- 2006. - Vol. 252. – No. 19. - P. 6609-6614.

Volandri G., Menichetti L., Matteucci M., Kusmic C., Consumi M, Magnani A., L’Abbate A., Landini L., Positanof V. An image formation model for Secondary Ion Mass Spectrometry imaging of biological tissue samples // Applied Surface Science. - 2010. - Vol. 257. – No. 4. - P. 1267-1275.

Green F.M., Gilmorea I.S., Seah M.P. G-SIMS and SMILES: Simulated fragmentation pathways for identification of complex molecules, amino acids and peptides // Applied Surface Science. - 2008. - Vol. 255. – No. 4. - P. 852-855.

Aoyagi S., Inoue M. An orientation analysis method for protein immobilized on quantum dot particles // Applied Surface Science. - 2009 - Vol. 256. – No. 4. - P. 995-997.

Hellweg S., Jacob A., Hoheisel J.D., Grehl T., Arlinghaus H.F. Mass spectrometric characterization of DNA microarrays as a function of primary ion species // Applied Surface Science. - 2006. - Vol. 252. – No. 19. - P. 6742-6745.

Zheng L., McQuaw C.M., Baker M.J., Lockyer N.P., Vickerman J.C., Ewing A.G., Winograd N. Investigating lipid–lipid and lipid–protein interactions in model membranes by ToF-SIMS // Applied Surface Science. - 2008. - Vol. 255. – No. 4. - P. 1190-1192.

Chandra S. Subcellular imaging of RNA distribution and DNA replication in single mammalian cells with SIMS: the localization of heat shock induced RNA in relation to the distribution of intranuclear bound calcium // J. of Microscopy. - 2008. - Vol. 232. – No. 1. - P. 27-35.

Amaya K.R., Monroe E.B., Sweedler J.V., Clayton D.F. Lipid imaging in the zebra finch brain with secondary ion mass spectrometry // Int. J. of Mass Spectrometry. - 2007. - Vol. 260. – No. 2-3. - P. 121-127.

Cole M.A., Jasieniak M., Thissen H., Voelcker N.H., Griesser H.J. Time-of-flight-secondary ion mass spectrometry study of the temperature dependence of protein adsorption onto poly (N-isopropylacrylamide) graft coatings. // Anal Chem. - 2009. - Vol. 81. – No. 16. - P. 6905-6912.

Tantsyrev G.D., Povolotskaja M.I. Vlijanie strukturi polimernih molekul na vtorichno-emiccionnie macc-spektri. Sbornik “Vzaimodeistvie atomnyh chastic s tverdim telom”. Kharkov. – 1976. – S. 18-21.

Pokrovskii V.A., Mosin V.V. Fast atom bombardment in mass spectrometry: Method and applications // Theoretical and Experimental Chemistry. – 1987. – Vol. 23. – No. 1. – P. 58-73.

Barber M., Bordoli R.S., Elliott G.J., Sedgwick R.D., Tyler A.N. Fast atom bombardment mass spectrometry // Anal. Chem. - 1982. - Vol. 54. – No. 4. - P. 645A-657A.

Bertrand P., Delcorte A., Garrison B.J. Molecular SIMS for organic layers: new insights // Applied Surface Science. - 2003. - Vol. 203-204. - P. 160-165.

Surman D.J., Vickerman J.C. Fast atom bombardment quadrupole mass spectrometry // J. Chem. Soc., Chem. Commun. - 1981. – No. 7. - P. 324-325.

Benninghoven A. Some aspects of secondary ion mass spectrometry of organic compounds // Int. J. of Mass Spectrometry and Ion Physics. - 1983. - Vol. 53. - P. 85-99.

Adriaensen L., Vangaever F., Lenaerts J., Gijbels R. Comparative study of organic dyes with time-of-flight static secondary ion mass spectrometry and related techniques // J. of Mass Spectrometry. - 2005. - Vol. 40. – No. 5. - P. 615-627.

Takayama M., Tanaka Y., Nomura T. Competitive formation of M+˙ and [M + H]+ ions under fast atom bombardment conditions // Organic Mass Spectrometry. - 1993. - Vol. 28. – No. 12. - P. 1529-1535.

Melnykov V.N., Litvinov V.A., Koppe V.T., Bobkov V.V., Tovstyak V.V. Issledovanie gljukozooksidazy metodom MSVI s ispol'zovaniem glicerinovoj matricy // Vzaimodejstvie ionov s poverhnost'ju (VIP-2005). Mater. XVII Mezhdunar. konf. M.: Izd. MAI, 2005. – Т. 1. – S. 411-414.

Melnykov V.N., Litvinov V.A., Koppe V.T., Bobkov V.V., Tovstyak V.V. Concentration effects in secondary ion emission for solutions of organic dyes in glycerol // 4th European Workshop on Secondary Ion Mass Spectrometry "SIMS Europe 2004". – Münster, September 26-29, 2004. – P. 91.

Melnykov V.N., Litvinov V.A., Koppe V.T., Bobkov V.V, Koppe V.T., Tovstyak V.V. Issledovanie koncentracionnyh effektov pri analize rastvorov organicheskih krasitelej v glicerine metodom VIMS//Vzaimodejstvie ionov s poverhnost'ju (VIP-2005). Mater. XVII Mezhdunar. konf. M.: Izd. MAI, 2005. – Т. 1. – C. 415-418.

Melnykov V.N., Litvinov V.A., Koppe V.T., Bobkov V.V., Tovstyak V.V. Issledovanie processov, protekajushhih v glicerinovyh rastvorah organicheskih krasitelej pri MSVI analize // Visnyіk HNU. Ser. Fizychna "Jadra, chastinkі, polja". – 2005. – No. 657. – S. 63-68.

Melnykov V.N., Litvinov V.A., Koppe V.T., Bobkov V.V., Alimov S.S. Some aspects of SIMS investigations of organic materials with use of glycerol matrix // Proc. of the 22nd International Conference on Atomic Collisions in Solids. 21st to 26th July 2006, Berlin. – P. 59.

Mel’nikov V.N., Litvinov V.A., Koppe V.T., Bobkov V.V. Secondary Ion Mass Spectrometry of Organic Materials with the Use of a Glycerol Matrix // Russian Journal of Physical Chemistry A. – 2009. - Vol. 83. – No. 6, - P. 979-983.

Rich R.L., Myszka D.G. Survey of the year 2007 commercial optical biosensor literature // J. of Molecular Recognition. - 2008. - Vol. 21. – No. 6 - P. 355-400.

Vo-Dinh T., Cullum B. Biosensors and biochips: advances in biological and medical diagnostics // Fresenius' J. of Anal. Chem. - 2000. - Vol. 366. – No. 6-7. - P. 540-551.

Mehrvar M., Bis C., Scharer J.M., Moo M., Luong J. H. Fiber-Optic Biosensors-Trends and Advances // Analytical Sciences. - 2000. - Vol. 16. – No. 7. - P. 677-692.

Kong T., Chen Y., Ye Y., Zhang K., Wang Z., Wang X. An amperometric glucose biosensor based on the immobilization of glucose oxidase on the ZnO nanotubes // Sensors and Actuators B: Chemical. - 2009. - Vol. 138. – No. 1. - P. 344-350.

Nenkova R., Ivanova D., Vladimirova J., Godjevargova T. New amperometric glucose biosensor based on cross-linking of glucose oxidase on silica gel/multiwalled carbon nanotubes/polyacrylonitrile nanocomposite film // Sensors and Actuators B: Chemical. - 2010. - Vol. 148. – No. 1. - P. 59-65.

Melnikov V.N., Litvinov V.A., Koppe V.T., Bobkov V.V. SIMS Study of the Processes in Buffer Solutions of Bioorganic Systems // Bulletin of the Russian Academy of Sciences: Physics. - 2008. - Vol. 72. – No. 7. - P. 929-933.

Melnikov V.N., Litvinov V.A., Koppe V.T., Bobkov V.V., Tovstyak V.V. Ob ispol'zovanii metoda MSVI dlja issledovanija vzaimodejstvija komponentov biosensora na gljukozu // Vestnik HNU. Ser. "Jadra, chasticy, polja". - 2006. – No. 721. - S. 95-100.

Melnykov V.N., Litvinov V.A., Koppe V.T., Bobkov V.V., Tovstyak V.V. SIMS study of processes in a system modeling glucose biosensor // 5th European Workshop on Secondary Ion Mass Spectrometry "SIMS Europe 2006", Münster, 2006. - P. 81.

Melnikov V.N., Litvinov V.A., Koppe V.T., Bobkov V.V. Issledovanie processov v bufernyh rastvorah bioorganicheskih sistem metodom MSVI // Tr. XVIII Mezhdunar. Konf. VIP. M.: Izd-vo MAI, 2007. - Т. 2. - С. 185-188.

Melnykov V.N., Litvinov V.A., Koppe V.T., Bobkov V.V. SIMS investigations of multicomponent bioorganic systems // 5th European Workshop on Secondary Ion Mass Spectrometry "SIMS Europe 2006", Münster, 2006. - P. 21.

Melnykov V.N., Litvinov V.A., Koppe V.T., Gamayunova L.A., Tovstyak V.V. SIMS investigations of Glucose Oxidase, Rhodamine 6G and Glucose Oxidase + Rhodamine 6G system in the glycerol matrix // 4th European Workshop on Secondary Ion Mass Spectrometry "SIMS Europe 2004", Münster, 2004. - P. 95.

Davydova M.E., Kurova V.S., Suhacheva M.V., Kupletskaja M.B., Riabov A.D., Netrusov A.I. Stabil'nost' i kataliticheskie svojstva gljukozooksidazy iz penicillium funiculosum G-15 // Vestn. Mosk. univ-ta. Ser.2 Himija. - 2002. - Т. 43. – No. 6. - S. 366-370.

Levi A., Sikevic S. Struktura i funkcii kletki. – M.: Mir, 1971. – 584с.

Peters D., Hayes J., Hieftje G. Chimicheskoe razdelenie i izmerenie. Teorija i praktika analiticheskoj chimii. – M.: Chimija, 1978. – 816 s.

Litvinov V.A., Koppe V.T., Bobkov V.V., Gamayunova L.А. Radiation damages of biological objects during SIMS studies // 6th European Workshop on Secondary Ion Mass Spectrometry "SIMS Europe 2008", Münster, 2008. - P. 81.

Litvinov V.A., Koppe V.T., Logachev Yu.E., Bobkov V.V. SIMS Investigation of the Influence of Ultraviolet Irradiation on Amino Acids // Bulletin of the Russian Academy of Sciences: Physics. - 2010. - Vol. 74. – No. 2. - P. 183-187.

Keough T., Ezra F.S., Russell A.F. et al. Comparison of the stable products formed by fast atom bombardment and γ-irradiation of glycerol // Org. Mass Spectrom. – 1987. – Vol. 22. – No. 5. – P. 241-247.

Litvinov V.A., Koppe V.T., Logachev Ju.E., Bobkov V.V. Issledovanie metodom VIMS vlijanija ul'trafioletovogo obluchenija na aminokisloty // Tr. XIX Mezhdunar. Konf. VIP. M.: Izd-vo MAI, 2009. - Т. 1. - С. 410-413.

Zenkevich I.G., Ioffe B.V. Interpretacija mass-spektrov organicheskih soedinenij. – Leningrad: Himija, 1986. 176 s.

Henry M., Dupont-Gillain C., Bertrand P. Conformation change of albumin adsorbed on polycarbonate membranes as revealed by ToF-SIMS // Langmuir. - 2003. - Vol. 19. - P. 6271-6276.

Benninghoven A., Jaspers D., Sichtermann W. Secondary ion emission of amino acids // Applied Physics B. - 1976. - Vol. 11. – No. 1. - P. 35-39.

Tamaki S., Sichtermann W., Benninghoven A. Static SIMS Investigation of Ag-Supported Amino Acids // Jpn. J. Appl. Phys. - 1984. - Vol. 23. - P. 544-549.